iiinova — where your digital self keeps evolving

Benjamin B Johnson @benjaminbjohnson.bsky.social · 13/12/2024

How to fix a broken #heart 💔->❤️ #Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes. Happy #Fluorescencefriday #BlueSky & #CardioSky! @ablanchasensio.bsky.social 👀 #Science 💥🧪 #Microscopy 🔬

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

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Dylan Burnette @mag2art.bsky.social · 11/12/2024

DNA during cell death and cell division. #Science #Biology #CellBiology #microscopy

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Albert Blanch-Asensio @ablanchasensio.bsky.social · 06/12/2024

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes.
A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below!
https://doi.org/10.1016/j.crmeth.2022.100300
https://doi.org/10.1101/2024.10.17.616637

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Maik Bischoff @maikbischoff.bsky.social · 27/11/2024

There are so many #morphogenesis researchers here! My first starter pack is full! Here is Part 2: go.bsky.app/K6YYKeC #devbio #cellbio #science Tell me if I should add you but try to make sure, that you're not already in Part 1. I cannot cross-reference everyone 😅 Part 1: go.bsky.app/RzDD9or

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 06/12/2024

Dedicating this #Fluorescencefriday to the #ECMatrix lovers. Was trialing some ECM staining methods on a scratch wound and love the HABP staining looking almost like flames 🔥🧪🔬 Lets see what you got, @ablanchasensio.bsky.social 😉

Cyan cellular extracellular matrix fibers are highlighted laying over the top of the orange hyaluronan secreted matrix after a scratch would assay.

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 05/12/2024

Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬 Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️ hPSC Disease Modeling: go.bsky.app/EpWsxNG For a good general stem cell pack go here: go.bsky.app/UTW4d7b

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 04/12/2024

🔥Super excited to drop our recent preprint🔥 biorxiv.org/cgi/content/... Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️ #Cardiosky #Science #hiPSC #Arrhythmia #Stemcell

Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.

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Albert Blanch-Asensio @ablanchasensio.bsky.social · 29/11/2024

Hey #BlueSky! Here is my first #FluorescenceFriday post! This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬. 🔵 Cardiomyocytes 🔴 Endothelial cells 🟡 Fibroblasts More coming next week! 🧪 #Science #Biology #CellBiology #SciArt @benjaminbjohnson.bsky.social

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Alexandre Dumoulin @axonsalex.bsky.social · 20/11/2024

My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks! go.bsky.app/KYYr7xc #liveimaging #livevellimaging #microscopy

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Danielle Beckman @daniellebeckman.bsky.social · 21/11/2024

105 cool microscopists in this starter pack, but I know there are more out there! I want to find more 45 to complete the list, can you help me? 🤓🔬 go.bsky.app/Aaf8sKT

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Joachim Goedhart @joachimgoedhart.bsky.social · 11/11/2024

Here's a starter pack that focuses on engineers/builders/makers/enablers of optical tools for (cell) biology. I'm pretty sure that the pack is incomplete - if you want to join the list, please reply with your favorite probe (that you made or used) and I'll add you 🤓 go.bsky.app/2YVvYER

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 22/11/2024

Hello #BlueSky! Figured my first post had to be a #FluorescenceFriday 🧪 I work on #hiPSC cardiac disease modeling ❤️ Image shows the initial collision and fusion of a #Cardioid (right) with a blood vessel organoid (left) as part of a fun collaboration with @mvilacuenca.bsky.social!

Confocal microscopy shows a mini 3D heart (Cardioid) as a sphere full of wavy orange, reds, and cyan in the process of colliding and merging with a blood vessel organoid filled with deep reds and dark blues.

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Albert Blanch-Asensio @ablanchasensio.bsky.social · 20/11/2024

Please share! Here is a starter pack dedicated to Mammalian SynBio! It is still incomplete so if you would like to be added/removed please let me know! go.bsky.app/SsxHKoV

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