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Benjamin B Johnson

@benjaminbjohnson.bsky.social

1.9K followers 3.3K following 41 posts

Early Career Postdoctoral Researcher at LUMC working with Christine Mummery and Richard Davis. 🔸 NWO-XS and Marie Curie Postdoctoral Fellowship grant winner 🔸 Stem Cell Disease Models 🔸 Myocardial Infarction and Cardiac Fibrosis #FluorescenceFriday

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 13/12/2024

How to fix a broken #heart 💔->❤️ #Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes. Happy #Fluorescencefriday #BlueSky & #CardioSky! @ablanchasensio.bsky.social 👀 #Science 💥🧪 #Microscopy 🔬

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 06/12/2024

Dedicating this #Fluorescencefriday to the #ECMatrix lovers. Was trialing some ECM staining methods on a scratch wound and love the HABP staining looking almost like flames 🔥🧪🔬 Lets see what you got, @ablanchasensio.bsky.social 😉

Cyan cellular extracellular matrix fibers are highlighted laying over the top of the orange hyaluronan secreted matrix after a scratch would assay.

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 04/12/2024

🔥Super excited to drop our recent preprint🔥 biorxiv.org/cgi/content/... Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️ #Cardiosky #Science #hiPSC #Arrhythmia #Stemcell

Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.

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Benjamin B Johnson @benjaminbjohnson.bsky.social · 22/11/2024

Hello #BlueSky! Figured my first post had to be a #FluorescenceFriday 🧪 I work on #hiPSC cardiac disease modeling ❤️ Image shows the initial collision and fusion of a #Cardioid (right) with a blood vessel organoid (left) as part of a fun collaboration with @mvilacuenca.bsky.social!

Confocal microscopy shows a mini 3D heart (Cardioid) as a sphere full of wavy orange, reds, and cyan in the process of colliding and merging with a blood vessel organoid filled with deep reds and dark blues.

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