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Alejandro Manzano Marín

@amanzanom.bsky.social
1.9K followers 485 following 328 posts

Senior Scientist 👨🏽‍🔬 @dome-vienna.bsky.social, @univie.ac.at 🇲🇽🇪🇺(🇦🇹🇫🇷🇪🇸) Symbionts, bacteria, insects, leeches, viruses, bioinformatics, evolution, genomics. Opinions are my very own. #SymbioSky besymblab.univie.ac.at

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Alejandro Manzano Marín @amanzanom.bsky.social · 06/10/2026
New #genome 🚨! Chromosomal reference for the 🐢 #leech #Placobdella costata ("C4" sp.). A small 👣 towards understanding this species complex, its #evolution, biol, & 🦠 symbionts! 🔗 doi.org/10.12688/ope... @biogeneurope.bsky.social @fwf-at.bsky.social @cemess.bsky.social @sangerinstitute.bsky.social
Picture of a Placobdella costata leech extending as it starts to move on a solid surface
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Alejandro Manzano Marín @amanzanom.bsky.social · 30/09/2026
how does one check/try this out??? Im interested....
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Alejandro Manzano Marín @amanzanom.bsky.social · 28/09/2026
Coevolutionary diversification between #hosts and their nutritional #symbionts in #phytophagous #hemipterans doi.org/10.1016/j.co... #SymbioSky
doi.org
Redirecting
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Alejandro Manzano Marín @amanzanom.bsky.social · 28/09/2026
A #transcriptionFactor regulates development of a bacteria-housing eukaryotic cell through DNA damage mitigation by the Luan lab (not in here...) doi.org/10.1073/pnas... #SymbioSky
doi.org
A transcription factor regulates development of a bacteria-housing eukaryotic cell through DNA damage mitigation | PNAS
Intracellular microorganisms are widespread and housed within specialized host cells called bacteriocytes in many invertebrates. Although increasin...
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Alejandro Manzano Marín @amanzanom.bsky.social · 18/09/2026
Please help spread the word! I'm searching for a #PhD student to join my group to work on #Symbiont - #pathogen interactions in #Hemiptera. Fully-funded, 4 years *Full details 👇🏼 besymblab.univie.ac.at/wp-content/u... 🧵 on the project 👇🏼 bsky.app/profile/aman... #ScienceJobs #SymbioSky #MicroSky
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Alejandro Manzano Marín @amanzanom.bsky.social · 29/08/2026
Cool. We find similar things in our leech genomes (need to wrap up that work soon...), which makes sense. A lot of evolutionary preadaptations facilitating the jump to blood feeding. Nice to see this in other animal systems. On my reading/citing list
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Andrew Mongue @ajmongue.bsky.social · 29/08/2026
New Mongue lab publication alert! The first first-author paper for master's student @ethangrebler.bsky.social who started this work as an undergraduate in the lab! The genome assembly of the ambush bug Phymata mystica! Read about it here: academic.oup.com/g3journal/ad... Highlights below 1/5
An ambush bug seen from above
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Alejandro Manzano Marín @amanzanom.bsky.social · 08/08/2026
Facultative #mutualism between #Paramecium and the intracellular #Rickettsiales bacterium #Megaera mediated by a horizontally acquired #biotin operon @isme-microbes.bsky.social doi.org/10.1093/isme... #SymbioSky
doi.org
Facultative mutualism between Paramecium and the intracellular Rickettsiales bacterium Megaera mediated by a horizontally acquired biotin operon
Abstract. The Rickettsiales are an alphaproteobacterial lineage engaging in ancient associations with a variety of eukaryotic hosts and with a wide spectru
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Alejandro Manzano Marín @amanzanom.bsky.social · 02/08/2026
Chingón! Added to #SymbioSky
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/07/2026
We will soon be advertising several positions, including PhD positions in both Krakow and Vienna. So, if the topic interests you, keep an eye out for the advertisements #ScienceJobs 6/6
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/07/2026
We expect the results of this project will uncover hidden #pathogen #diversity, clarify how microbial partners shape disease spread, and provide a foundation for predicting pathogen #emergence and developing innovative strategies to mitigate #vector-borne plant diseases. 5/6
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/07/2026
Finally, we will approach the experimental validation of inferred positive/negative interactions between #pathogens and the co-occurring facultative #symbionts. 4/6
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/07/2026
We will carry out high-throughput analyses of thousands of samples in order to infer #symbiont composition as well as identify potential pathogenic taxa carried by the sampled #insects. We will then perform functional and genomic characterisation of selected insect populations. 3/6
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/07/2026
Within this project, we will explore potential #pathogens carried by #hemipteran #insects (namely #aphids, #psyllids, and true #hoppers) and the the interaction of these with facultative #symbiont strains. 2/6
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/07/2026
Very happy to that our large-scale collaborative weave project (w/ @annamichalik.bsky.social & @sympiotr.bsky.social ) "Plant pathogens in #insect #microbiome [...]" has been approved! @fwf-at.bsky.social @ncn.gov.pl @univie.ac.at @jagiellonskiuni.bsky.social @dome-vienna.bsky.social 1/6 👇🏽
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Alejandro Manzano Marín @amanzanom.bsky.social · 13/07/2026
Last week, I had the pleasure to host a speed visit of Mariia Shrestkha to talk about Branchiobdellida, little #leech -like animals..... Discussed project ideas and evolution of these particular little #worms! Excited for the science to come!
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/07/2026
Intrahost mutational dynamics parallel long-term #genomeEvolution in #endosymbionts by @symboevo.bsky.social & Younghwan Kwak doi.org/10.1093/molb... #SymbioSky
doi.org
Intrahost mutational dynamics parallel long-term genome evolution in endosymbionts
Abstract. Obligate endosymbionts of insects undergo extreme genome evolution, marked by accelerated molecular evolution, severe base-pair compositional bia
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Nancy Moran @nancy-moran.bsky.social · 02/07/2026
A step forward in discovering Buchnera's key to the aphid's heart, published today! Thanks to Jerry Maeda and other authors--a great team -- and to the helpful reviewers #SymbioSky doi: doi.org/10.1038/s415...
doi.org
A secreted endosymbiont protein essential for colonizing host cells - Nature
SyeA was present in the Buchnera ancestor, is secreted into the host cytoplasm, is homologous to secreted effectors of bacterial pathogens and is essential for Buchnera transmission.
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Alejandro Manzano Marín @amanzanom.bsky.social · 30/06/2026
Proposed terminology for referring to the homes of #symbionts within their ( #insect ) hosts " #Symbiotic organs in #insects: diversity, functional implications, and terminology " w/ @kaltenpoth-lab.bsky.social and co., not in bsky doi.org/10.1098/rstb... #SymbioSky
doi.org
Symbiotic organs in insects: diversity, functional implications, and terminology
Abstract. With over a million described species, insects represent the most successful group of animals on Earth. One of the drivers of insect diversity is
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Alejandro Manzano Marín @amanzanom.bsky.social · 07/06/2026
SypC, a #symbiont #outerMembraneVesicle protein, impacts the development of the #squid – #vibrio partnership doi.org/10.1073/pnas... #SymbioSky
doi.org
SypC, a symbiont outer membrane vesicle protein, impacts the development of the squid–vibrio partnership | PNAS
Bacterial outer membrane vesicles (OMVs) and the cargo they carry are increasingly recognized as a means of communication between microbial symbion...
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Mart Krupovic @mkrupovic.bsky.social · 11/05/2026
With Eugene Koonin, we wrote a rather comprehensive review on the origin, evolution and organization of the #virosphere. We describe all 10 viral realms and the logic behind them, and so much more. Check it out! comptes-rendus.academie-sciences.fr/biologies/ar...
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Alejandro Manzano Marín @amanzanom.bsky.social · 31/05/2026
#Genome-resolved #metagenomics reveals conserved, flexible and emerging #symbioses across global #leafhoppers doi.org/10.64898/202... #SymbioSky
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Hugo Bisio @molbiolgv.bsky.social · 29/05/2026
Just published! Check out our review of the Nucleocytoviricota infection cycle. Great teamwork! @mkrupovic.bsky.social @willemsenanouk.bsky.social @fischerlab.bsky.social @puffy-virus.bsky.social
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Anouk Willemsen @willemsenanouk.bsky.social · 22/05/2026
New Work out! A multilayered network reveals the centrality of newly discovered #Nucleocytoviricota in wastewater treatment plant communities w/ @dominikluecking.bsky.social & @amanzanom.bsky.social doi.org/10.64898/202... @erc.europa.eu @univie.ac.at @dome-vienna.bsky.social 1/4
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Alejandro Manzano Marín @amanzanom.bsky.social · 18/05/2026
.. Thus, we show that these symbionts have coevolved under comparable selective constraints and demographic events at least within this clade of Cinara species, that originated ~25 Million years ago
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Alejandro Manzano Marín @amanzanom.bsky.social · 18/05/2026
.. And no evidence of relaxed selection on protein-coding genes shared between Buchnera and Serratia, suggesting that the presence of Serratia does not exacerbate genome erosion in Buchnera even on redundant metabolic genes! ...
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Alejandro Manzano Marín @amanzanom.bsky.social · 18/05/2026
.. To our surprise, both bacterial lineages show similar substitution rates, contrasting what we have observed in, for example, Leafhopper's symbiotic systems (Nasuia and Sulcia). Genome variations within and between aphid species indicate strong purifying selection acting on both symbionts..
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Alejandro Manzano Marín @amanzanom.bsky.social · 18/05/2026
#Aphids typically rely on the obligate #symbiont #Buchnera, but several lineages have acquired additional co-obligate symbionts, most commonly #Serratia symbiotica. Through phylogenetic analyses, we found that a clade of Cinara aphids shows strict cospeciation between aphids and both endosymbionts..
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Alejandro Manzano Marín @amanzanom.bsky.social · 18/05/2026
New work out! #Coevolution and synchronized evolutionary rates in #aphid dual #endosymbiot sytems w/ Jess, Emmanuelle, Armelle, & co. doi.org/10.64898/202... @cbgpmontpellier.bsky.social @inrae-france.bsky.social @cemess.bsky.social @dome-vienna.bsky.social @univie.ac.at #SymbioSky #MicroSky
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Rob Edwards @linsalrob.bsky.social · 06/05/2026
Want to find #prophage in your #bacteria genomes? We built PhiSpy a long time ago, and now we've created a new web page for it: linsalrob.github.io/PhiSpyWeb/ Best of all, the data never leaves your computer! #phage #phagesky
linsalrob.github.io
PhiSpyWeb – Browser-based Prophage Prediction
Browser-based prophage prediction using PhiSpy, Pyodide, and WebAssembly
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Alejandro Manzano Marín @amanzanom.bsky.social · 12/05/2026
#gutMicrobiota of Brazilian #Melipona stingless #bees A distinct microbiota architecture retaining a compartment-specific specialization, suggesting an "alternative symbiotic strategy that may reflect unique dietary ecology and evolutionary history." doi.org/10.1371/jour... #SymbioSky
doi.org
Gut microbiota of Brazilian Melipona stingless bees: Dominant members and their localization in different gut regions
The gut microbiome of eusocial corbiculate bees, which include honeybees, bumblebees, and stingless bees, consists of anciently associated, host-specific bacteria that play crucial role in nutrition, ...
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Alejandro Manzano Marín @amanzanom.bsky.social · 06/05/2026
Pentapartite symbiotic system in #Legendrea loyezae by @filiphusnik.bsky.social & co. doi.org/10.21203/rs.... #SymbioSky #protistsOnSky
doi.org
A novel mitochondrion-related organelle and complex endosymbiosis in a rare anaerobic single-celled predator
Anaerobic single-celled eukaryotes illustrate the remarkable diversity of adaptations to life without oxygen, including multiple mitochondrial reductions and various symbiotic interactions with prokar...
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Megan Sorensen @messorensen.bsky.social · 30/04/2026
Interested in working on the evolution of photosynthetic endosymbioses? Come join us! I have 2 open positions in my new group @cemess.bsky.social in Vienna 3yr postdoc info: tinyurl.com/4emkjpn4 4yr PhD info: tinyurl.com/mtxmsjpy Apply by 1st June! Please share/repost #Protists #Symbiosis #PhD
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Alejandro Manzano Marín @amanzanom.bsky.social · 04/05/2026
Same here
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Alejandro Manzano Marín @amanzanom.bsky.social · 17/04/2026
Missed this one when it came out. #Genome degradation results in nested #symbiosis and #endosymbiont replacement in #cicadas doi.org/10.1038/s414... #SymbioSky
doi.org
Genome degradation results in nested symbiosis and endosymbiont replacement in cicadas - Nature Communications
The evolutionary mechanisms leading to symbiont replacement in insects are not well understood. Zhou et al. show that genome degradation results in nested symbiosis and endosymbiont replacement in cic...
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Alejandro Manzano Marín @amanzanom.bsky.social · 15/04/2026
Cellular remodeling of ovarian follicular epithelial cells transmits an obligate nutritional #endosymbiont in a #scaleInsect #Insignibacter tubulum, novel nutritional endosymbiont of #Insignorthezia insignis doi.org/10.1073/pnas... #SymbioSky
doi.org
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
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Alejandro Manzano Marín @amanzanom.bsky.social · 01/04/2026
arre!
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Richard Sever @richardsever.bsky.social · 31/03/2026
Elsevier costs were ~£1.7B in 2025 newsletter.journalology.com/p/elsevier-2... Compare that to arXiv 👇
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Enrico Sandro Colizzi @escolizzi.bsky.social · 26/03/2026
How common is multicellularity in bacteria? And archaea? And how does it evolve? We wrote a short review "On the architecture and evolution of prokaryotic multicellularity". Preprint link: bit.ly/4ta06Gq Sharing and comments are much appreciated. 1/4
An overview of bacterial multicellular formations: biofilms, filaments, free-floating aggregates, motile collectives and fruiting bodies. For each form, we mention an analogous eukaryotic multicellular form (respectively animal epitelia, filaments in fungi, Volvox, Dictyostelium/social animals, Dictyostelium and other slime moulds)
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Maria Dzunkova @dzunkovam.bsky.social · 24/02/2026
Leeches are so cool! 🤩 We had a special speaker today at @i2sysbio.es , Alejandro Manzano Marin @amanzanom.bsky.social . I have known him since my PhD and I'm really amazed by his research line about symbionts of leeches #symbiosis #microsky
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
... or, maybe the transcriptional slippage correction is already a feature that is found early in these pseudogenisation process and they just end up getting fixed at the long term in x or y genes (maybe related to expression levels, as some of these are very "essential" genes)....
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
Several/Many of these HP-tract-containing genes have evolved repeatedly in independent lineages. So, I immediately wondered whether or not these pop out in your dataset. Maybe there is some specific tolerance for HP tracts and bias of correction at the transcription level in those genes...
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
While the first category I have commonly analysed more in small to tiny genomes, the second category I have commonly found also in "early" endosymbionts. Anyways, things that popped into my mind checking out your paper. I'm really into pseudogenes..... and your dataset is pretty cool
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
Also, I imagine another fraction might be those that have recorded homologues that can function in large to severely truncated forms (e.g. doi.org/10.1038/srep...). This paper includes just some of them, but I keep an extensive collection of these references in my Zotero library (I can share it...).
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
I especially wondered this in light of the very few proteins you detect from genes classified as pseudogenes. I imagine a fraction of these are homopolymeric frameshifted ones. You should be able to easily pinpoint them by identifying transcripts where the frameshift is corrected (transcr. slippage)
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
Sorry, copied it wrong doi.org/10.1073/pnas...
doi.org
Endosymbiont gene functions impaired and rescued by polymerase infidelity at poly(A) tracts | PNAS
Among host-dependent bacteria that have evolved by extreme reductive genome evolution, long-term bacterial endosymbionts of insects have the smalle...
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
Very cool work! I wonder though, as (I think) it is not mentioned in the paper: Do pseudogenes with polyA/T/etc tracts end up more commonly transcribed and/or translated? Thinking about 👇🏼 doi.org/10.1073/pnas... & doi.org/10.1093/molb... Does this start early or is is a late-symbiont thing only...
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Alejandro Manzano Marín @amanzanom.bsky.social · 09/02/2026
Regardless from the cut-offs, which can be affected by the amount of applications and the percentage of reviewers that are aware that if they want a certain grant funded, they should provide a (nearly) perfect score, let's not forget that those ridiculous comments come from fellow "scientists" 🫠
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Alejandro Manzano Marín @amanzanom.bsky.social · 30/01/2026
Yba3 (sorry, SyeA) was one of those enigmatic Buchnera proteins: (almost) always there, but who knows what it did.... Now we know! SyeA is "secreted and homologous to secreted effectors of bacterial pathogens AND is essential for Buchnera transmission"! Really cool work! (image: Ext. Data Fig 2.)
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Alejandro Manzano Marín @amanzanom.bsky.social · 30/01/2026
This is a really cool study on one of my favourite obligate symbiotic systems: Aphid + Buchnera by @jerrymaeda.bsky.social, @nancy-moran.bsky.social, & co. A secreted #endosymbiont protein (SyeA, formally Yba3) essential for colonizing host cells doi.org/10.1101/2025... #SymbioSky
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