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Light-Sheet Fluorescence Microscopy (LSFM) course

@lsfm-na.bsky.social
284 followers 250 following 33 posts

This is the US version of the long running course Pavel and Emmanuel have been running. We are just this side of the pond. Being held at MDIBL in Bar Harbor, Maine, June 2-6, 2025.

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Brandy A Freitas @brandy-syglass.bsky.social · 09/06/2025
This was a great sample to check out -- who knew 100M year old amber would be so close to Ethyl Cinnamate in RI? Huge shout out to @brukercorporation.bsky.social for the fleet of lightsheets at @lsfm-na.bsky.social last week!
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New England Society for Microscopy @nesmicroscopy.bsky.social · 09/06/2025
For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social ! 🔬: Bruker @brukercorporation.bsky.social, LCS SPIM 🟠: Courtesy the Sims Collection 🥼&🎥: Jan Frankowski @frankowskijc.bsky.social @lsfm-na.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 07/06/2025
And with that, #lsfm25 comes to a close! We want to thank everyone who helped make this possible - the faculty, course directors, TAs, vendors, and sponsors. But most importantly the many wonderful people at MDIBL. See you all next year!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 06/06/2025
Preparation for the final event of the course: a boat tour of Acadia!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 06/06/2025
Now that the students have had several days to learn sample preparation and image acquisition, we are wrapping up day five with a crash course called "Now what do you do with your data?" Led by @dpshepherd.bsky.social and Carsten Wolff. #lsfm25 #microscopy #science #education
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Doug Shepherd @dpshepherd.bsky.social · 04/06/2025
Spending some time at #lsfm25 cleaning up code. It now has distributed GPU gradient-consensus RL decon (@jamesdmanton.bsky.social @andrewgyork.bsky.social) our fast orthogonal deskew, and stitching/fusion to ome-ngff v0.5. Example AO-OPM data on uncleared mouse brain slice github.com/QI2lab/opm-p...
Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.
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Brandy A Freitas @brandy-syglass.bsky.social · 05/06/2025
Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday. 🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social 🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma... 🥽 syGlass.io @lsfm-na.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 05/06/2025
We are so excited to start day four with a wonderful talk from Kara Cerveny! #lsfm25 #science #microscopy #education @mdibiolab.bsky.social @kara-cerveny.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 04/06/2025
It's day three and we are starting our morning off with an excellent intro to clearing by Doug Richardson. #lsfm25 #microscopy #science #education @mdibiolab.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 04/06/2025
And it begins....
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 03/06/2025
The calm before the storm indeed! In just a half an hour, this board will be bombarded by 25 students rushing to grab a spot on their instrument of choice. 11 systems. Only 9 labs. May the odds be ever in their favor... #lsfm25 #microscopy #science #education @mdibiolab.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 03/06/2025
We are back from lunch with a talk from Raghav Chhetri telling us all about using light sheet microscopy for neuroscience imaging! #lsfm25 #microscopy #science #education @mdibiolab.bsky.social @rchhmiscope.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 03/06/2025
It's day two! We're starting off a day filled with science with a talk from Anna-Karin Gustavsson from Rice University. #lsfm25 #microscopy #science #education @mdibiolab.bsky.social @riceuniversity.bsky.social @gustavssonlab.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 03/06/2025
Morning in Bar Harbor doesn’t suck.
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Emmanuel G. Reynaud @ereynaud.bsky.social · 03/06/2025
Step 8: In @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Well day one done worth a sunset!
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Emmanuel G. Reynaud @ereynaud.bsky.social · 02/06/2025
Step @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Went to collect some samples... Here is a pink tunic ate!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
Reto Fiolka and OPM for the Keynote!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
With the end of our first round of vendor talks behind us, we are excited to welcome Reto Fiolka as our keynote speaker this year. #lsfm25 #microscopy #science #education @mdibiolab.bsky.social
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
Holly Gibbs discussing the logistics of image processing and analysis
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
While we are on our lunch break, the students are enjoying some beautiful weather here at @mdibiolab.bsky.social. #lsfm25 #microscopy #science #education
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
We now have Vera Morales from Nikon telling us all about objectives: the why, when, and how! #lsfm25 #microscopy #science #education
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
Claudette St Croix from CBI kicks off LSFM 2025 at @mdibiolab.bsky.social
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Melissa Cooper, PhD @melcooperphd.bsky.social · 02/06/2025
@lsfm-na.bsky.social Is this heaven, or am I just currently surrounded by ELEVEN DIFFERENT LIGHT SHEET MICROSCOPES? So thrilled to be out here in Acadia chatting about microscopy!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 02/06/2025
LSFM 2025 has kicked off with an introduction from the one and only, @glaevsky.bsky.social, our course director. #lsfm25 #microscopy #science #education
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 01/06/2025
Calm before the storm…
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 01/06/2025
Time to sheet!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 31/05/2025
Why no swim?!
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Emmanuel G. Reynaud @ereynaud.bsky.social · 31/05/2025
Step 2 : On my way to @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Queueing and sterile halls is next at Charles de Gaulle Airport !
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 31/05/2025
See you soon!
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Emmanuel G. Reynaud @ereynaud.bsky.social · 03/04/2025
Registration prices are going down but quality will be the same!
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 03/04/2025
12 systems will be there, Bruker x3!, 3i, Leica, ASI, MBF, LiT, LifeCanvas x2!, and Miltenyi! Amazing cutting-edge technology, accessible instructors, and Bar Harbor! Please re-bluesky (what do you call it here:))!
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Emmanuel G. Reynaud @ereynaud.bsky.social · 27/03/2025
And join a community, ready to share, to help and to support you... Already 5th course in Europe and 4th in the US ( @lsfm-na.bsky.social )...
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MDI Biological Laboratory @mdibl.org · 12/02/2025
Join us at MDI Bio Lab (@mdibl-lmf.bsky.social) for an exciting 2025 lineup of #microscopy courses designed for researchers & professionals — May 16-23, June 1-7, and Aug 5-13! #microscopymonday #fluorescentproteins #lightsheet 🧪 🤝 🧬 🐟🎸 🍎 Learn more & enroll now! mdibl.org/education/co...
mdibl.org
Courses
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 17/02/2025
Registration for LSFM at @mdibl-lmf.bsky.social 6/1-6/7 is now open! This is an amazing academic environment, and is unique that all the major vendors will be there and students are ENCOURAGED to bring their own samples! mdibl.org/course/lsfm-...
mdibl.org
Light Sheet Fluorescence Microscopy 2025
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3i @the.3i.social · 17/02/2025
If you are interested in an immersive week imaging on AxL CTLS, you should sign up for #LSFM2025 @lsfm-na.bsky.social. A great course with a fantastic new location @mdibiolab.bsky.social for 2025. #microscopy #clearedtissue #lightsheet 🌎 mdibl.org/course/lsfm-... 🔬 www.intelligent-imaging.com/AxL
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 17/02/2025
Registration for LSFM at @mdibl-lmf.bsky.social 6/1-6/7 is now open! This is an amazing academic environment, and is unique that all the major vendors will be there and students are ENCOURAGED to bring their own samples! mdibl.org/course/lsfm-...
mdibl.org
Light Sheet Fluorescence Microscopy 2025
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Special Ed @edwardlachica3.bsky.social · 23/01/2025
@ritastrack.bsky.social how does this ban on NIH comms affect you and yours?
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Emmanuel G. Reynaud @ereynaud.bsky.social · 02/12/2024
Now that I have settled here quietly, here is a new serie on microscope #microscopefoot and no it is not a fetishist nerd post but an exploration of the shape of the microscope foot. Let's start with Episode 1: "The round one". From Hooke supported microscope to Nachet, Vollensak ...
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Gary Laevsky @glaevsky.bsky.social · 26/11/2024
Thanks Ed. Here is the link to the course on this side of the pond @LSFM-na.bsky.social mdibl.org/course/lsfm-...
mdibl.org
Light Sheet Fluorescence Microscopy 2025 – MDI Biological Laboratory
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Special Ed @edwardlachica3.bsky.social · 22/11/2024
in 2021, I was a member of the team @the.3i.social that described, for the first time, using a #lightsheet #microscope, the full nervous system of a mouse. I don't know if this has ever been duplicated. Sample from CIBR, Dr. Hu Zhao. Happy #FluorescenceFriday Nerds. BB-PEG. 6.5X/0.25NA
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MDI Biological Laboratory @mdibl.org · 20/11/2024
Congratulations to Marko Pende and Hannah Somers! Marko’s image of a tissue-cleared ladybug won 1st place in the Evident 5th Annual Image of the Year Contest, and Hannah's green crab was awarded honorable mention. Both were created at our #LightMicroscopy #CoreFacility! 🧪🤝 mdibl.org/mdi-bio-lab-...
tissue-cleared ladybug
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MDI Biological Laboratory @mdibl.org · 19/11/2024
MDI Biological Laboratory was recently mentioned in a @mainepublic.org story on seagrass restoration efforts in Maine. Read how scientists are banding together to save these important #eelgrass habitats and battling this alarming trend 🧪🤝🖥️: www.mainepublic.org/climate/2024...
mainepublic.org
Scientists and community groups band together to save Casco Bay's eelgrass
Through a huge coalition effort, five organizations are working on a method to restore the bay's depleted eelgrass meadows.
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Oded Rechavi @odedrechavi.bsky.social · 19/11/2024
Hope I'm the first to post this all time classic on this platform
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Anna-Karin Gustavsson @gustavssonlab.bsky.social · 16/11/2024
Excited to have our work on a Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells featured on the cover of Biomedical Optics Express! doi.org/10.1364/BOE....
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Emmanuel G. Reynaud @ereynaud.bsky.social · 16/11/2024
Should I make a lightsheet microscopy starter pack? Who wants to join? @retof.bsky.social @edwardlachica3.bsky.social @astormic.bsky.social @olu-gh.bsky.social @adamkglaser.bsky.social @glaevsky.bsky.social
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Special Ed @edwardlachica3.bsky.social · 14/11/2024
Hey nerds, you know about this, right? Spots are filling up. You can still register using the QR code. And I’ll be there with my lightsheet microscope. 🤸‍♀️🤸‍♀️🤸‍♀️
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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · 17/11/2024
Here we are! @ereynaud.bsky.social
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