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Reposted by Beck Laboratory

Katharina Geißler @kgeissler.bsky.social · 16/12/2025

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

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Reposted by Beck Laboratory

SCALE - SubCellular Architecture of LifE @scale-cluster-ffm.bsky.social · 23/05/2025

🎉 We’re absolutely thrilled: SCALE has been selected as a DFG Excellence Cluster! A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de #ClustersOfExcellence #SCALEcluster 🧵

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Beck Laboratory @becklab.bsky.social · 11/04/2025

(3/3) We propose a model, where the NPC dynamically acts to buffer tension applied to the nuclear envelope, thereby protecting it from potential rupture. Aberrations of the NPC scaffold would cause a reduced buffering capacity and result in NPC disintegration and potential rupture.

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Beck Laboratory @becklab.bsky.social · 11/04/2025

(2/3) Nup133 is a conserved component of the Y-complex, its deletion however is surprisingly is not lethal, but causes tissue and cell-type specific defects. We found that NPCs in Nup133-/- cells over-stretch and disintegrate upon induction of neuronal differentiation.

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Beck Laboratory @becklab.bsky.social · 11/04/2025

(1/3) Together with the labs of Valerie Doye @vdoye.bsky.social and Hans-Georg-Kräusslich, we analyzed NPC structure in Nup133 null mESCs. We found heterogenous NPCs featuring non-canonical symmetries and missing subunits. Now online @naturecellbiology.bsky.social. www.nature.com/articles/s41...

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Reposted by Beck Laboratory

Sergio Cruz-León @sergiocruzleon.bsky.social · 11/04/2025

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

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Reposted by Beck Laboratory

Jan Philipp Kreysing @jpkreysing.bsky.social · 11/04/2025

New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇

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Beck Laboratory @becklab.bsky.social · 17/01/2025

Summarized, our data suggest a HIV-1 nuclear entry model where first a CypA coat around the capsid gets stripped away while the capsid is drawn into the FG-Nup mesh of NPC. Then the compression of the FG-Nups forces the NPC scaffold to crack allowing further progression of the capsid.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

Through MD simulations we show that capsids face a significant steric barrier inside the NPC, especially when the vast amount of intrinsically disordered FG-Nups in the central channel are included. This barrier could be relieved by a crack in the NPC.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

Through template matching NPC ring subunits, we were able to investigate structural changes to individual NPCs and found that capsid presence is significantly linked to NPCs being cracked open, meaning the subunit geometry exceeds the normal 8-fold rotational symmetry in the NPC scaffold.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

Using cryo-ET subtomogram averaging (#TeamTomo), we traced capsid (CA) lattices in virions, the cytoplasm, NPC, and nucleus. Cytoplasmic CA hexamers and pentamers showed bound density consistent with CypA, lost upon NPC entry. The CypA coat likely protects the capsid from host restriction factors.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

CLEM-ET data showed that morphologically intact cone-shaped capsids could be found inside the central channel of NPCs and also directly underneath in the nucleus further solidifying that capsids do not need to disassemble for nuclear entry.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

The HIV capsids were detected on the cytoplasmic side, inside the central channel, and on the nuclear side of the NPCs. These data suggest that passage through the NPC is a significant hurdle for the capsid.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

@jpkreysing.bsky.social, @maziarheidari.bsky.social, Vojtech Zila, and others studied HIV-1 capsid nuclear entry in primary human macrophages using STED, CLEM-ET, cryo-ET (#TeamTomo), and MD simulations. First, 3D STED revealed capsid signal accumulation at nuclear pores.

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Beck Laboratory @becklab.bsky.social · 17/01/2025

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

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Reposted by Beck Laboratory

Max Planck Institute of Biophysics @mpibp.bsky.social · 17/01/2025

Fresh out of the press! ✨Check out our new paper on the violent entry of the HIV capsid into the nuclear pore — and how it cracks the gate! 🤯 💥 Incredible work from @becklab.bsky.social and @hummerlab.bsky.social 🤩 More here: www.biophys.mpg.de/2842024/hivcapsid-cracks-the-npc?c=2011019 🔗 #teamTOMO

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