Justin Eyquem @j-eyquem.bsky.social · 04/02/20252/ We first show that Ark313 can efficiently transduce T cells in vivo, including naïve, memory and regulatory subsets, outperforming natural AAV serotypes (AAV5 and AAV6). 110
Justin Eyquem @j-eyquem.bsky.social · 04/02/20253/ With a single IV injection, Ark313 achieves gene delivery in up to 25% of T cells, in both circulating and tissues, including tissue resident memory! This unlocks new possibilities for studying T cell function in their natural niche. (🙏 to Roberto Ricardo Gonzalez and @arimolofskylab.bsky.social) 121
Justin Eyquem @j-eyquem.bsky.social · 04/02/20254/ We validated Ark313 for in vivo CRISPR gene editing. A single injection of Ark313 delivering a sgRNA in Cas9-expressing mice resulted in up to 50% KO of the transduced T cells—including tissue-resident cells. 120
