In 2020, we (Andrew Payne, Paul Reginato) showed in situ genome sequencing, which we used to reveal the 3D genome at the very first stages of life: www.science.org/doi/10.1126/...
However, the resolution of IGS is capped by nuclear volume and the diffraction limit of light
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science.org
In situ genome sequencing resolves DNA sequence and structure in intact biological samples
A technical approach allows simultaneous sequencing and imaging of genomes in human fibroblasts and early mouse embryos.
