1/n: 2nd preprint this week: www.biorxiv.org/content/10.1.... We functionalized a G(13)PCR P2RY8 that keeps germinal center (GC) B cells confined. Taylor LaFlam performed saturation mutagenesis, Aashish Manglik's lab solved the structure, and collaboration with Cyster lab validated variant effects.
biorxiv.org
Phenotypic pleiotropy of missense variants in human B cell-confinement receptor P2RY8
Missense variants can have pleiotropic effects on protein function and predicting these effects can be difficult. We performed near-saturation deep mutational scanning of P2RY8, a G-protein-coupled re...
