iiinova — where your digital self keeps evolving

Wildtype One @wildtypeone.bsky.social · 14/07/2026

Your passage number might be affecting your data more than you think❗ Liu et al. (2025) looked at RNA-seq data across passages P3–P39. (a thread)

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Wildtype One @wildtypeone.bsky.social · 11/07/2026

Before you repeat the experiment, answer this: What kind of problem do you actually have? Ugly data does NOT have one next step. (a thread)

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Wildtype One @wildtypeone.bsky.social · 08/07/2026

“Everyone does it this way” is not a valid statistical defense. (1/3)

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Wildtype One @wildtypeone.bsky.social · 07/07/2026

“Do more replicates until it’s significant” is killing your credibility 🧫 (a thread)

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Wildtype One @wildtypeone.bsky.social · 30/06/2026

Clear cell death. p = 0.056. Now what?

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Wildtype One @wildtypeone.bsky.social · 18/06/2026

Answer the following question honestly: (a thread)

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Wildtype One @wildtypeone.bsky.social · 22/05/2026

The biggest discount your lab will ever get? (a thread 🔬💰👇)

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Wildtype One @wildtypeone.bsky.social · 09/05/2026

Don't always trust expiry labels on lab reagents (at least if you're in a rush) Here's proof from a wild (and true) story 👇 (1/5)

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Wildtype One @wildtypeone.bsky.social · 05/05/2026

It uses the same samples as Western blots. But instead of taking 2 days to reveal 2 proteins... Kinome profiling reveals all 400+ major signaling kinases ...in two hours. (1/5)

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Wildtype One @wildtypeone.bsky.social · 03/05/2026

Your work isn’t wrong. It might just be early. 🧫

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Wildtype One @wildtypeone.bsky.social · 02/05/2026

The last year of the PhD/postdoc is NOT "just another year." It is when everything concludes. Like the last episode of a series. (a thread)

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Wildtype One @wildtypeone.bsky.social · 01/05/2026

According to 2025 data, you can cut 60% of your flow failures with 4 fixes only (see thread 👇)

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Wildtype One @wildtypeone.bsky.social · 30/04/2026

Scan your manuscript—fraud can be unintentional (a thread)

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Wildtype One @wildtypeone.bsky.social · 28/04/2026

13 cases where your experiments disagree & what it means (a thread 👇)

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Wildtype One @wildtypeone.bsky.social · 26/04/2026

Before you start your week tomorrow, remember... 🥼👇

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Wildtype One @wildtypeone.bsky.social · 25/04/2026

Contamination rarely looks like contamination Here are unusual signs we often ignore 🚩👇 (a thread)

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Wildtype One @wildtypeone.bsky.social · 21/04/2026

The fastest guide to fix Western blots—8 failures in 30 second Save for emergencies 🚨 (A thread👇)

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Wildtype One @wildtypeone.bsky.social · 20/04/2026

Stop using SEM because it’s cleaner than SD (see thread)

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Wildtype One @wildtypeone.bsky.social · 27/03/2026

Imagine Einstein had an hour to fix your experiment 👴🏻 🧬 (a thread)

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Wildtype One @wildtypeone.bsky.social · 21/03/2026

If your experiments worked but your figures still feel fragile, it’s often not the biology—it’s the analysis. This webinar walks through the 5 most common statistics mistakes biologists make + how to avoid them No coding. No equations. Just clarity. 👉 Register for the webinar here: wildtypeone.com

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Wildtype One @wildtypeone.bsky.social · 11/03/2026

“Everyone does it this way” is not a valid statistical defense. (a thread)

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Wildtype One @wildtypeone.bsky.social · 08/03/2026

While irreproducibility is undermining the biggest research breakthroughs We're hearing more false data today than ever The literature is being taken with a pinch of salt This one phrase in the methods section gave a confidence boost for the whole article (1/4)

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Wildtype One @wildtypeone.bsky.social · 06/03/2026

Bench problems AI already solved—and what AI still cannot do 🥼 (a thread)

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Wildtype One @wildtypeone.bsky.social · 03/03/2026

How to attend? The booking is live now — and slots are available from March 16 to March 27 Book yours here 👉 calendly.com/wildtypeone/... See you live — Wildtype One 🧬

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Wildtype One @wildtypeone.bsky.social · 25/02/2026

Pick TidyPlots (R ecosystem) if you’re comfortable learning R and want full control over statistics, plotting grammar, and large datasets, while accepting a steeper learning curve and slower onboarding for new lab members. (4/n)

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Wildtype One @wildtypeone.bsky.social · 25/02/2026

Pick BioVinci if you want an intuitive drag-and-drop interface with quick visualizations for clustering and small-to-medium datasets, while accepting that it’s not built for very large files or highly complex statistical control. (3/n)

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Wildtype One @wildtypeone.bsky.social · 25/02/2026

Pick XLSTAT (Excel add-in) if you already live in Excel and want direct access to statistical tools inside familiar spreadsheets, while accepting dependence on Excel’s stability and limited scalability for big data. (2/n)

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Wildtype One @wildtypeone.bsky.social · 25/02/2026

Pick Prism (GraphPad) if your lab runs routine statistical analyses and wants fast, clean, citable figures without coding, while accepting higher yearly license costs and limitations for large omics-scale datasets. (1/n)

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Wildtype One @wildtypeone.bsky.social · 25/02/2026

Researchers, don't wing your statistics 📊—here's the best software for your lab 🔬 (a thread)

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Wildtype One @wildtypeone.bsky.social · 11/02/2026

All of the following do NOT count as sufficient controls (by lab technique) (a thread)

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Wildtype One @wildtypeone.bsky.social · 10/02/2026

A tip from over a decade of flow cytometry experience: (a thread)

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Wildtype One @wildtypeone.bsky.social · 07/02/2026

Researchers, Flow data feels objective, until two people gate it (1/4)

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Wildtype One @wildtypeone.bsky.social · 30/01/2026

FlowJo should not be the default! 🙅‍♂️ How can you choose the best flow cytometry software for your case? Here's your rule of thumb: (1/9)

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Wildtype One @wildtypeone.bsky.social · 28/01/2026

Researchers, don't choose the default just because everyone uses it. Here's how to pick the ideal flow software for your case (see today's post on LinkedIn 👇) www.linkedin.com/posts/wildty...

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Wildtype One @wildtypeone.bsky.social · 27/01/2026

Researchers, If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change (1/3)

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Wildtype One @wildtypeone.bsky.social · 25/01/2026

Quick tip 🔬🧑‍🔬 If you do Western blots, immunofluorescence, or any technique that requires antibody staining Remember: Overnight incubation at 4°C is not always the best protocol ⚠️ (1/2)

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Wildtype One @wildtypeone.bsky.social · 21/01/2026

Do you use SEM because it looks cleaner? SEM vs. SD = one of the most quietly damaging figure-making habits in biology Let’s clear it up (a thread)

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Wildtype One @wildtypeone.bsky.social · 18/01/2026

Researchers, Most irreproducible results don’t fail loudly They quietly rot (a thread)

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Wildtype One @wildtypeone.bsky.social · 16/01/2026

Sadly, only 11-25% of the biology literature is reproducible But there's a solution... (a thread)

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Wildtype One @wildtypeone.bsky.social · 14/01/2026

Reviewers will ask you to repeat experiments... Only because of small details that you didn't include How do you avoid losing time and repeating experiments? 🤔 (a thread)

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Wildtype One @wildtypeone.bsky.social · 13/01/2026

Could your cell line be the wrong one for your disease? 🧫 (a thread)

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Wildtype One @wildtypeone.bsky.social · 11/01/2026

Everyone’s thinking it. Few say it out loud. The literature looks confident - Clean figures - Tight conclusions - Cropped blots that “say enough” (1/n)