Wildtype One @wildtypeone.bsky.social · 14/07/2026Your passage number might be affecting your data more than you think❗ Liu et al. (2025) looked at RNA-seq data across passages P3–P39. (a thread) 100
Wildtype One @wildtypeone.bsky.social · 11/07/2026Before you repeat the experiment, answer this: What kind of problem do you actually have? Ugly data does NOT have one next step. (a thread) 100
Wildtype One @wildtypeone.bsky.social · 08/07/2026“Everyone does it this way” is not a valid statistical defense. (1/3) 100
Wildtype One @wildtypeone.bsky.social · 07/07/2026“Do more replicates until it’s significant” is killing your credibility 🧫 (a thread) 110
Wildtype One @wildtypeone.bsky.social · 26/06/202626 HARD lab truths biology researchers take years to learn: 1. You don't have to respond to all reviewer comments. Some will harm your project. ... (a thread) 100
Wildtype One @wildtypeone.bsky.social · 18/06/2026Answer the following question honestly: (a thread) 110
Wildtype One @wildtypeone.bsky.social · 22/05/2026The biggest discount your lab will ever get? (a thread 🔬💰👇) 100
Wildtype One @wildtypeone.bsky.social · 12/05/2026🧬 DNA found no candidate resistance mechanism in 51% of resistant patients. 📃 mRNA explained only ~40% of protein-abundance variation. 🧱 Protein abundance explained only ~10% of enzyme-activity variation. One omic layer is not the whole truth. Omics need YOUR judgment. — Wildtype One 000
Wildtype One @wildtypeone.bsky.social · 09/05/2026Don't always trust expiry labels on lab reagents (at least if you're in a rush) Here's proof from a wild (and true) story 👇 (1/5) 100
Wildtype One @wildtypeone.bsky.social · 05/05/2026It uses the same samples as Western blots. But instead of taking 2 days to reveal 2 proteins... Kinome profiling reveals all 400+ major signaling kinases ...in two hours. (1/5) 100
Wildtype One @wildtypeone.bsky.social · 05/05/2026Biologists lose some of their work's value when they chase aesthetics: - SEM instead of SD - Dynamite plots everywhere - Not showing sample and effect sizes The biology is in the nuance. 🧬 Catch yourself chasing aesthetics. Show the variability. 000
Wildtype One @wildtypeone.bsky.social · 03/05/2026Your work isn’t wrong. It might just be early. 🧫 000
Wildtype One @wildtypeone.bsky.social · 02/05/2026The last year of the PhD/postdoc is NOT "just another year." It is when everything concludes. Like the last episode of a series. (a thread) 101
Wildtype One @wildtypeone.bsky.social · 01/05/2026According to 2025 data, you can cut 60% of your flow failures with 4 fixes only (see thread 👇) 100
Wildtype One @wildtypeone.bsky.social · 30/04/2026Scan your manuscript—fraud can be unintentional (a thread) 100
Wildtype One @wildtypeone.bsky.social · 29/04/2026Stop making “clean” figures that hide your data. Bar charts compress ❌ SEM hides variability ❌ One chart ≠ every experiment ❌ Good figures show: • all points (small n) • distribution (large n) • direction (paired data) If your figure looks clean but hides truth, it’s not good science. 📊 000
Wildtype One @wildtypeone.bsky.social · 28/04/202613 cases where your experiments disagree & what it means (a thread 👇) 110
Wildtype One @wildtypeone.bsky.social · 26/04/2026Before you start your week tomorrow, remember... 🥼👇 020
Wildtype One @wildtypeone.bsky.social · 25/04/2026Contamination rarely looks like contamination Here are unusual signs we often ignore 🚩👇 (a thread) 100
Wildtype One @wildtypeone.bsky.social · 23/04/2026Never buy an antibody because of: - Brand ❌ - Price ❌ - Number of citations ❌ Only buy antibodies if: It has been validated for your exact application and species ✅ When buying antibodies, validation is king. It saves you time, money, and failed experiments. 010
Wildtype One @wildtypeone.bsky.social · 22/04/2026To all hard-working researchers: Working long hours & weekends does NOT prove that you're serious. No one cares how long you work. Exhausted work = less depth = worse judgment We're paid to use our thinking brains. Brain performance requires recharging. — Wildtype One 🧬 020
Wildtype One @wildtypeone.bsky.social · 21/04/2026The fastest guide to fix Western blots—8 failures in 30 second Save for emergencies 🚨 (A thread👇) 100
Wildtype One @wildtypeone.bsky.social · 20/04/2026Stop using SEM because it’s cleaner than SD (see thread) 100
Wildtype One @wildtypeone.bsky.social · 18/04/2026Western blots are great for focused questions. But are not discovery tools. Too many failure modes. Only semi-quantitative. Technically fragile. Use high-throughput methods for discovery. Use western blots to validate. Don't invert the sequence. 010
Wildtype One @wildtypeone.bsky.social · 13/04/2026You can remove outliers without feeling guilty—under one condition only: If it's a "T-case." What's a T-case? 🤔 Read today's Wildtype Weekly to find out (in 3 minutes or less 👇 )linkedin.comYou can remove outliers—under one condition“If you torture the data long enough, it will confess to anything.” — Ronald Coase Dear Researcher, We’ve all had unusual data that: didn’t reproduce “ruined” our figures caused statistics to be “non-... 010
Wildtype One @wildtypeone.bsky.social · 27/03/2026Imagine Einstein had an hour to fix your experiment 👴🏻 🧬 (a thread) 100
Wildtype One @wildtypeone.bsky.social · 21/03/2026If your experiments worked but your figures still feel fragile, it’s often not the biology—it’s the analysis. This webinar walks through the 5 most common statistics mistakes biologists make + how to avoid them No coding. No equations. Just clarity. 👉 Register for the webinar here: wildtypeone.com 020
Wildtype One @wildtypeone.bsky.social · 17/03/2026Irreproducibility undermines the biggest research breakthroughs And we hear more false data today than ever Below are 7 Method-section phrases that can kill doubt and have people trust your work All in 3 minutes or less—read today's Wildtype Weekly 👇linkedin.com7 small manuscript lines that kill doubt“We are drowning in information, while starving for knowledge.” — Sydney Brenner Dear Researcher, This one phrase in a 2026 Method section gave a confidence boost for the whole article: Irreproducibil... 000
Wildtype One @wildtypeone.bsky.social · 11/03/2026“Everyone does it this way” is not a valid statistical defense. (a thread) 100
Wildtype One @wildtypeone.bsky.social · 09/03/2026AI still struggles to replace researchers because of one trait that machines still lack Researchers who use it consistently reach world-class levels 🏆 What is this trait? Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)linkedin.comYour one trait that AI can’t replace yet“Computers are useless. They can only give you answers. 000
Wildtype One @wildtypeone.bsky.social · 08/03/2026While irreproducibility is undermining the biggest research breakthroughs We're hearing more false data today than ever The literature is being taken with a pinch of salt This one phrase in the methods section gave a confidence boost for the whole article (1/4) 111
Wildtype One @wildtypeone.bsky.social · 06/03/2026Bench problems AI already solved—and what AI still cannot do 🥼 (a thread) 120
Wildtype One @wildtypeone.bsky.social · 03/03/2026Good news: you don’t always have to repeat the experiment — because your next figure might be hiding in your old data (a thread)linkedin.comHow to avoid failed statistics after solid experiments (1-min note)“You must not fool yourself — and you are the easiest person to fool.” — Richard Feynman Dear Researcher, For us, the most frustrating thing in the lab isn’t even failed experiments It is: Experiments... 100
Wildtype One @wildtypeone.bsky.social · 25/02/2026Researchers, don't wing your statistics 📊—here's the best software for your lab 🔬 (a thread) 110
Wildtype One @wildtypeone.bsky.social · 23/02/2026Imagine your experiment worked—but it’s invalid because you didn't the correct control! How do you avoid choosing the wrong control? Ready today's Wildtype Weekly to learn more (in 3 minutes or less 👇)linkedin.comThe controls that force you to repeat experiments“A theory can never be proven; it can only be falsified.” — Karl Popper Dear Researcher, Nothing is more frustrating than this: Your experiment worked. 010
Wildtype One @wildtypeone.bsky.social · 16/02/2026Part 2/2—let’s finish the list! 3 pro-tips from a decade of flow experience (in 3 minutes or less) Read today's Wildtype Weekly 👇linkedin.com“Titration is not a suggestion” (+2 other tips)“With great power comes great responsibility.” — Uncle Ben from Spider-Man Dear Researcher, Last week, Juliano Freitas, Ph. 010
Wildtype One @wildtypeone.bsky.social · 11/02/2026All of the following do NOT count as sufficient controls (by lab technique) (a thread) 110
Wildtype One @wildtypeone.bsky.social · 10/02/2026A tip from over a decade of flow cytometry experience: (a thread) 100
Wildtype One @wildtypeone.bsky.social · 09/02/2026We all had to repeat failed experiments because our first design wasn't optimal. How to avoid this in flow cytometry? Read Juliano's six panel design pro-tips in 3 minutes or less in today's Wildtype Weekly 👇linkedin.comDesign flow panels without crying later (3 rules, 3 min)“If the facts don’t fit the theory, change the facts.” — Albert Einstein Dear Researcher, Technology is powerful But it will punish ignorant scientists Weeks ago, Wildtype One talked to Juliano Freita... 000
Wildtype One @wildtypeone.bsky.social · 07/02/2026Researchers, Flow data feels objective, until two people gate it (1/4) 110
Wildtype One @wildtypeone.bsky.social · 03/02/2026Humble, precise language can make your lab work shine But when describing their lab work, researchers often misuse the same 10 words Are you using them too? Read the Wildtype Weekly to find out (in 3 minutes or less 👇 )linkedin.comStop using these words in your lab work“Data are not facts; they are claims that must be justified.” — John Tukey Dear Researcher, This article will improve your presentations and manuscripts in 3 minutes or less Not by changing the data B... 010
Wildtype One @wildtypeone.bsky.social · 30/01/2026FlowJo should not be the default! 🙅♂️ How can you choose the best flow cytometry software for your case? Here's your rule of thumb: (1/9) 110
Wildtype One @wildtypeone.bsky.social · 28/01/2026Researchers, don't choose the default just because everyone uses it. Here's how to pick the ideal flow software for your case (see today's post on LinkedIn 👇) www.linkedin.com/posts/wildty... 000
Wildtype One @wildtypeone.bsky.social · 27/01/2026Researchers, If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change (1/3) 110
Wildtype One @wildtypeone.bsky.social · 26/01/2026Remember: Peer review is a human process, and some comments can harm your work 🔬 Wildtype One looked at true stories from researchers We found 10 reviewer comments you should absolutely ignore Read today's Wildtype Weekly to find them out 👇linkedin.comIgnore these 10 reviewer comments“What reviewers should be doing is judging whether the conclusions follow from the premises—not whether they would have done the work differently.” — Sydney Brenner, Nobel Prize Laureate Dear Research... 010
Wildtype One @wildtypeone.bsky.social · 25/01/2026Quick tip 🔬🧑🔬 If you do Western blots, immunofluorescence, or any technique that requires antibody staining Remember: Overnight incubation at 4°C is not always the best protocol ⚠️ (1/2) 101
Wildtype One @wildtypeone.bsky.social · 21/01/2026Do you use SEM because it looks cleaner? SEM vs. SD = one of the most quietly damaging figure-making habits in biology Let’s clear it up (a thread) 262
Wildtype One @wildtypeone.bsky.social · 19/01/2026Many cell lines in research aren't what researchers think they are 🧫 So, how would a world-class scientist troubleshoot their project? Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)linkedin.comIn case you chose the wrong cell line…“Ignorance more frequently begets confidence than does knowledge.” — Charles Darwin Dear Researcher, Could your cell line be the wrong one for your project? Dr. 010
Wildtype One @wildtypeone.bsky.social · 18/01/2026Researchers, Most irreproducible results don’t fail loudly They quietly rot (a thread) 110