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Pranav Seth @thebuddhabird93.bsky.social · 25/09/2026

🔬🐦 Zebra finch retina! AAV-mediated GCaMP expression reveals elongated photoreceptors 🟢 GCaMP | 🔴 DAPI | ⚪ Transmission: distinctive oil droplets in the photoreceptor layer These droplets contribute to spectral filtering #FluorescenceFriday #ZebraFinch #Retina #Vision #GCaMP #Neuroscience

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Pranav Seth @thebuddhabird93.bsky.social · 07/07/2026

Two days into #FENS2026 and I'm loving it! Great science, inspiring discussions, and lots of new ideas. Around on Friday (10th of July, 09:30–13:00)? Come by PS07-10AM-034: "Viral Strategies for 2-Photon Imaging of Avian Retinal Circuits." #Retina #Birds #2PhotonImaging #AAV

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Pranav Seth @thebuddhabird93.bsky.social · 03/07/2026

Excited for #FENS2026! 🇪🇸 I'll be presenting "Viral strategy for two-photon imaging of avian retina" during Poster Session 07 (Fri 10 July, 09:30–13:00). I develop AAV-based tools for functional imaging of avian retinal circuits. See you in Barcelona! 🧠 #FENS2026 #Neuroscience #TwoPhotonImaging #AAV

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Pranav Seth @thebuddhabird93.bsky.social · 03/07/2026

Guess it's time for another #FluorescenceFriday ✨ A constellation of green lights in the #Zebrafinch #retina. #AAV-delivered #GCaMP8m illuminates #photoreceptors, turning living neuroscience into art while revealing the hidden language of #songbird retinal #circuitry. 🔴 DAPI 🟢 GCaMP8m

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Pranav Seth @thebuddhabird93.bsky.social · 29/05/2026

Back again with another #FluorescenceFriday retina post because the zebra finch retina still looks unreal under the microscope. A tiny layered neural circuit built for fast and precise vision in these small #songbirds. #ZebraFinch #Retina #Neurobiology

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Pranav Seth @thebuddhabird93.bsky.social · 22/05/2026

#FluorescenceFriday ✨ A glimpse into the beautifully layered architecture of the zebra finch retina highlighting the remarkable stratification of retinal cells and circuits from two different perspectives: 🔬 First image: a side view

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Pranav Seth @thebuddhabird93.bsky.social · 24/04/2026

#Zebrafinch retina stained with a calcium marker, revealing a tightly layered neural map beneath a simple looking tissue sheet. Built for life in bright open Australian habitats, their visual system is tuned for speed, precision, and rapid decision making. #FluorescenceFriday #Retina

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Pranav Seth @thebuddhabird93.bsky.social · 03/04/2026

Looks like a cosmic explosion… but it’s actually a #zebrafishretina 🔬 Pancone line expressing syGCaMP6f Confocal, whole-mount prep Sometimes biology doesn’t need enhancement to look spectacular. #FluorescenceFriday #Neuroscience #Retina

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Pranav Seth @thebuddhabird93.bsky.social · 20/03/2026

For today’s #FluorescenceFriday, the #rods clearly chose chaos What you’re looking at are long, beautifully coiled outer segments of rod #photoreceptors… basically the retina showing off its inner spaghetti aesthetic. Elegant, tangled, and absolutely essential for seeing in low light.

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Pranav Seth @thebuddhabird93.bsky.social · 19/03/2026

One year since my paper “AAV-mediated transduction of songbird retina” was published in Frontiers in Physiology. It’s been great to see it reach ~3,400 views and ~740 downloads across a global audience. Really grateful to everyone involved in this work. www.frontiersin.org/journals/phy...

frontiersin.org

Frontiers | AAV-mediated transduction of songbird retina

IntroductionGenetic manipulation of murine retinal tissue through ocular administration of adeno-associated viruses (AAVs) has become a standard technique to...

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Pranav Seth @thebuddhabird93.bsky.social · 27/02/2026

Although the world outside still feels grey and wintry, under the microscope the retina reveals a vibrant landscape of color. For #FluorescenceFriday: a section of the avian retina showing beautifully organized neuronal layers from inner to outer retina. #Neuroscience #Retina #Microscopy

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RoordaLab @roordalab.bsky.social · 24/02/2026

Vision Science makes the front page of NYT!! featuring work from @neurofishh.bsky.social and @denilsson.bsky.social with comments from Berkeley's own @karthikshekhar.bsky.social #visionscience www.nytimes.com/2026/02/23/s...

nytimes.com

The Rise of Eyes Began With Just One

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Pranav Seth @thebuddhabird93.bsky.social · 18/02/2026

Early for #FluorescenceFriday but who cares.. Retinal neural networks in a frame Chicken retina whole mount showing amacrine cells, with their dendritic arborizations forming a connected mesh A reminder that the retina is a powerful neural circuit shaping vision before ganglion cells even fire

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Reposted by Pranav Seth

Andrew Iwaniuk @evoneuro.bsky.social · 26/01/2026

One of my favourite winter birds, a redpoll, checking things out below. #Alberta #birds #birding 🪶🇨🇦

A small greyish finch with a black chin, yellowish bill and a small red cap is perched on a curved branch. Its head is tilted to one side as it looks down at something below.

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Pranav Seth @thebuddhabird93.bsky.social · 23/01/2026

Old school markers, still strong 🟣 Calbindin labels double cone photoreceptors, bipolar cells, and ganglion cells. 🟢 Calretinin highlights horizontal cells, bipolar cells, and IPL stratification. Avian retina circuitry, clearly written in layers. #FluorescenceFriday #Neuroscience #Retina #Imaging

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Pranav Seth @thebuddhabird93.bsky.social · 08/01/2026

Two years since I first arrived at the beautiful #UniversityofSussex campus Joined as a #Postdoctoral Research Fellow in 2024. Grateful for the experiences, challenges, growth, and the journey so far. Cheers to what’s ahead.

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Pranav Seth @thebuddhabird93.bsky.social · 16/12/2025

Attended and presented a poster at the wonderful 2 day #ASABWinter2025 meeting in the beautiful city of #Edinburgh. A coming together of researchers asking how sensory worlds shape #AnimalBehaviour. A genuine sense of community across generations of animal ethologists. @asab.org

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Pranav Seth @thebuddhabird93.bsky.social · 25/11/2025

My parents, Retd Prof R K Seth and Retd Prof Dr Ranjana Seth, published a new research paper today. Even at 69, they continue to push scientific boundaries. Watching them work with such dedication is one of my biggest inspirations. Link: www.tandfonline.com/eprint/HRTTZ...

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Pranav Seth @thebuddhabird93.bsky.social · 20/11/2025

Revisiting some older material and this paper came up again during a discussion about viral tools in avian retina. Our study was part of the Rising Stars in Avian Physiology collection and showed that AAV2 9 can reliably reach and transduce avian photoreceptors. More in thread below..

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Pranav Seth @thebuddhabird93.bsky.social · 14/11/2025

Ending my #FluorescenceFriday with this beautiful avian retina image depicting the beautiful stratification in the avian eyes. This is where complexity meets art. #retina #avian #bird #plexiformlayer

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Pranav Seth @thebuddhabird93.bsky.social · 06/11/2025

#FluorescenceFriday A sky of light within ✨ This is the ganglion cell layer of the avian retina. The nerve fiber layer lies beneath like a soft green current, carrying quiet signals forward. Sometimes to see the universe with its beautiful scattered stars, one only has to look inside.

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Pranav Seth @thebuddhabird93.bsky.social · 06/11/2025

Turns out zebrafish cones are frenemies. Red/UV see and Green/Blue suppress...

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Pranav Seth @thebuddhabird93.bsky.social · 31/10/2025

Layers of vision, glowing green #FluorescenceFriday Here, #photoreceptors expressing a #glutamate biosensor mark the first step in visual processing, with a single inner-retina #neuron (likely amacrine) revealing deeper circuit activity before signals ever reach the brain. #Neuroscience #Microscopy

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Pranav Seth @thebuddhabird93.bsky.social · 24/10/2025

#FluorescenceFriday 🌿🔬 post: Avian retina transduced with my AAVs carrying the #GluSNFR biosensor. Bright blobs at the top mark photoreceptor terminals; meanwhile below, an RGC connects beautifully with bipolar cells, giving a glimpse of retinal circuitry in action. #Retina #Neuroscience #AAV #2-P

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Pranav Seth @thebuddhabird93.bsky.social · 09/10/2025

Thrilled to share that my paper “AAV-mediated transduction of songbird retina” (Frontiers in Physiology, 19 Mar 2025) is featured in the official Rising Stars in Avian Physiology 2024 e-book! 🐦🔬 🔗 www.frontiersin.org/research-top... #AvianPhysiology #FrontiersInPhysiology

frontiersin.org

Rising Stars in Avian Physiology: 2024

We are delighted to present the second Frontiers in Avian Physiology Research Topic "Rising Stars in Avian Physiology." The goal of this topic is to recognize future leaders in avian physiology who ar...

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BWJones @bwjones.bsky.social · 27/09/2025

Day 2 #Visionarium2025 @teuler.bsky.social talks about What is a cell type?

Thomas Euler speaking in front of a projected image.

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Pranav Seth @thebuddhabird93.bsky.social · 24/09/2025

Presented my poster “AAV-based Strategy for 2P Imaging in the Avian Retina” at #ERM2025 in Pécs 🇭🇺. I am grateful for inspiring discussions on retinal circuitry, photoreceptors, regeneration & artificial vision, and for connecting with colleagues in #RetinaResearch & #Neuroscience.

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Pranav Seth @thebuddhabird93.bsky.social · 19/09/2025

✨🔬 #FluorescenceFriday Confocal stack of chicken retina 🐥 — from inner nerve fibers up to photoreceptors. Layer upon layer of mosaics, each cell type in its place. Birds have remarkable visual systems, and under the microscope, they look absolutely stunning in detail.

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Pranav Seth @thebuddhabird93.bsky.social · 19/09/2025

How do birds see the world — and how can we study it at the cellular level? 🐦 Six months ago, our paper came out in Frontiers in Physiology with a new tool to answer exactly that. Here’s a little thread...🧵

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Pranav Seth @thebuddhabird93.bsky.social · 12/09/2025

Read up this brilliant paper by @gkafetzis.bsky.social on how retina evolution is so conserved.. or is it? 🤓

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Pranav Seth @thebuddhabird93.bsky.social · 12/09/2025

#FluorescenceFriday #Seahorse retina stained with #GNB3 (green) + #DAPI (cyan). GNB3 labels cone photoreceptors + ON #bipolarcells, tracing pathways from outer to inner retina. Amazing to see conserved visual #circuits in this non-traditional model

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Pranav Seth @thebuddhabird93.bsky.social · 04/09/2025

#FluorescenceFriday The #seahorse #retina shows off its beauty — stunning stratification in the inner nuclear and inner #plexiform layers, with rod #bipolar terminals forming a brilliant band. #Vertebrate retinas: the same basic plan, yet each species adds its own twist. Its so fascinating.

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Pranav Seth @thebuddhabird93.bsky.social · 29/08/2025

Seahorse retina in full color! This is a whole-mount of the seahorse #retina, labeled with Calretinin, Calbindin, and PKCα revealing its intricate architecture. Each marker highlights different neural circuits, showing how these remarkable creatures process the world around them. #FluorescenceFriday

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Pranav Seth @thebuddhabird93.bsky.social · 25/07/2025

#FluorescenceFriday Throwback to my #PhD: EGFP-labeled photoreceptors in a #Europeanrobin retina after AAV2/9 delivery Rods, cones, even a tiny horizontal cell. Lots of trial and error—but we got there. First of its kind in #songbirds. 📄 doi.org/10.3389/fphy... Still proud of it.

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Pranav Seth @thebuddhabird93.bsky.social · 14/07/2025

#MicroscopyMonday AAV injections in chick retina: #photoreceptors transduced as expected—plus widespread #Müllerglia labeling across layers! Shows AAV’s promise for multi-cell targeting in vision repair. 📄 doi.org/10.3389/fphy... #AAV #Retina #GeneTherapy

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Pranav Seth @thebuddhabird93.bsky.social · 10/07/2025

✨ #FluorescenceFriday update (coming early—conference-bound!)✨ Here’s a look at the stunning stratification of Photoreceptors in the chicken retina, captured using immunostaining and AAV-mediated gene delivery. 🟢 GFP, 🔴 PSD95, 🔵 DAPI It’s gratifying to see the biology light up like this—literally.

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Pranav Seth @thebuddhabird93.bsky.social · 04/07/2025

Orthogonal slice of a confocal Z-stack from a whole-mounted #avian #retina, highlighting the laminar relationship between the nerve fiber layer ( #NFL ) and underlying #RGCs. Imaged with Leica #SP8, processed in Fiji. #FluorescenceFriday #BirdRetina #Neuroscience #ConfocalImaging

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Pranav Seth @thebuddhabird93.bsky.social · 23/06/2025

Here is a cool post for #MicroscopyMonday. Here's a 3D visualization from my recent project using #SP8 confocal #microscopy. Red = DAPI nuclear staining Green = Photoreceptor transduction via AAV serotypes with reporter/ #biosensor genes This image shows successful targeting of photoreceptors.

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Pranav Seth @thebuddhabird93.bsky.social · 20/06/2025

#FluorescenceFriday 🐥:3D projection of #GluSnFR expression in #chick #retina following #AAV transduction. Expression is concentrated in the #photoreceptor layer. 🟢 GluSnFR (glutamate sensor) 🔴 DAPI-stained nuclei #Imaging #AAV #Retina #Biosensor #neuron #vision (doi.org/10.3389/fphys.2025.1549585)

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Pranav Seth @thebuddhabird93.bsky.social · 23/05/2025

The beautiful single cone #Photoreceptor types in the #retina of #chicken expressing #GFP via my #Intravitreal AAV injections. For more insight, check out my recent publication (www.frontiersin.org/journals/phy...) Great images for #FluorescenceFriday. #birds #AAVs #injections #transduction

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Pranav Seth @thebuddhabird93.bsky.social · 19/05/2025

Pleased to share an exciting update on our recent publication “AAV-mediated transduction of songbird retina” lnkd.in/eeu_q5aF In two months, it attracted: 1,158 views and 259 downloads. This strong engagement reflects growing interest in #genetictools for #avian models in #vision #neuroscience.

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Pranav Seth @thebuddhabird93.bsky.social · 16/05/2025

Sharing another beautiful image of a #Gallus gallus ( #chicken ) #retina, stained with various markers, taken by me for #FluorescenceFriday What better way to start a Friday. Retina is a beauty.

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Pranav Seth @thebuddhabird93.bsky.social · 06/05/2025

Happy to share that my latest research paper titled "AAV-mediated #transduction of #songbird #retina" which was published in the journal "Frontiers in Physiology", in its special issue "Rising Stars in Avian Physiology: 2024", reached a #1000 views in less than 2 months. lnkd.in/e_8yQtKA

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Pranav Seth @thebuddhabird93.bsky.social · 05/04/2025

One of my recent articles, published in Frontiers in Physiology, has just reached a new impact milestone. It got a 100 downloads in 17 days. Here is the link if you would like to read our study: www.frontiersin.org/article/1549...

frontiersin.org

Frontiers | AAV-mediated transduction of songbird retina

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Reposted by Pranav Seth

SFB 1372 @sfb1372.bsky.social · 26/03/2025

Congrats to our researchers around @thebuddhabird93.bsky.social on their latest joint publication: Using a custom-designed injection setup, they identified a #viral #serotype with the capability to successfully induce widespread transduction of the bird retina. doi.org/10.3389/fphy...

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Pranav Seth @thebuddhabird93.bsky.social · 19/03/2025

I am delighted to present to all my latest research paper titled " #AAV-mediated transduction of #songbird #retina" which has been published in the journal " #Frontiers in #Physiology", in its special issue "Rising Stars in Avian Physiology: 2024". #birds #AAV www.frontiersin.org/journals/phy...

frontiersin.org

Frontiers | AAV-mediated transduction of songbird retina

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Migration Department, Max Planck Institute of Animal Behavior @animaltracking.bsky.social · 18/03/2025

ICARUS BIRDS Where, how, and why do birds die? We are launching the ICARUS BIRDS project to find out! Join us in tracking common birds across Europe to uncover the factors behind their decline. Learn more on our website: www.ab.mpg.de/icarus

ab.mpg.de

ICARUS Projects

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Pranav Seth @thebuddhabird93.bsky.social · 16/03/2025

Another beauty that I came across during my #2-photon experiments is a Polyaxonal amacrine cell (PAC), a type of #interneuron in the #retina with multiple axon-like processes, said to play a crucial role in visual signal processing and modulation, particularly in the #IPL.

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Migration Ecology Group @migecol.bsky.social · 03/03/2025

We are happy to introduce our lab members - today: Annika @annikapeter.bsky.social! She studies songbird navigation skills for her PhD & is fascinated by all kinds of birds & enjoys observing their behaviour, both at work & in her free time. Wheatears have a special place in her heart ♥️

@annikapeter.bsky.social with a Wheatear tagged and to be released for a scientifc study

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