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Structura Biotechnology @structurabio.bsky.social · 29/09/2026

🇨🇦 Last week, we wrapped up “Beyond Consensus Refinements: Advanced Cryo-EM Data Processing with #CryoSPARC” in Toronto! Thank you to all the #cryoEM scientists who joined us, and to @sickkidsto.bsky.social for another great collaboration! We already look forward to the next workshop! Stay tuned!

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Structura Biotechnology @structurabio.bsky.social · 24/09/2026

🇨🇦 Today we kicked off our hands-on workshop, “Beyond Consensus Refinements: Advanced Cryo-EM Data Processing with CryoSPARC,” in collaboration with The Hospital for Sick Children @sickkidsto.bsky.social! Excited for two days of diving deeper into advanced #cryoEM data processing with #CryoSPARC. 🔬

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Structura Biotechnology @structurabio.bsky.social · 23/09/2026

🔎 A classic #cryoEM particle curation conundrum: 2D or 3D classification? "Decoy Classification" using Heterogeneous Refinement in #CryoSPARC curates particles directly in 3D, helping remove junk particles while preserving valuable views. Read more in the CryoSPARC Blog 👉 bit.ly/3SXfwS0

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Structura Biotechnology @structurabio.bsky.social · 16/09/2026

🚀 Register now for the 2026 Stanford-SLAC CryoEM Center (S2C2) Data Processing Workshop, December 9-11! We’re excited to contribute as #CryoSPARC instructors to this hybrid workshop, together with instructors from CryoDRGN, EMAN2 and AreTomo! More info and registration 👉 bit.ly/4gXbnXd

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Structura Biotechnology @structurabio.bsky.social · 10/09/2026

💡 The idea is simple: getting to know your data is often the shortcut to more efficient particle picking. In our latest #CryoSPARC blog post, we cover how to speed up #cryoEM data processing through efficient early curation of your dataset. 👉 cryosparc.com/blog/curatio...

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Structura Biotechnology @structurabio.bsky.social · 03/09/2026

🚀 Next week we will be at the 2026 New England #Cryo-EM Symposium at MIT, showcasing recent #CryoSPARC developments. If you're attending, we'd love to connect! cryoem.mit.edu/2026-ne-cryo...

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Structura Biotechnology @structurabio.bsky.social · 01/09/2026

💊 The Pain Paradox: Activating TRPV1 to Silence Its Response - Researchers leveraged rational drug design, functional experiments, and #cryoEM to characterize a new capsaicin-inspired TRPV1 agonist with prolonged analgesic effects. Read more in the #CryoSPARC Blog 👉 cryosparc.com/blog/trpv1-m...

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Structura Biotechnology @structurabio.bsky.social · 27/08/2026

🛠️ Even the most carefully managed IT environments need to be prepared for the unexpected. In #CryoSPARC v5, we introduced Instance Recovery, a built-in recovery system for unforeseen database loss or corruption, adding another layer of resilience to our #cryoEM software. Read more 👉 bit.ly/4xlZMGS

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Structura Biotechnology @structurabio.bsky.social · 20/08/2026

🔬Malaria Invasion at Molecular Resolution: From Protein Inhibitors to Membrane Remodeling In a recent Cell publication, researchers at Columbia University resolved the endogenous structure of the malaria moving junction staple using #cryoEM. Read more in the #CryoSPARC Blog 👉 bit.ly/46aX6jl

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Structura Biotechnology @structurabio.bsky.social · 17/08/2026

We also benchmark our methods against established metrics (including cryoEF, Fourier Shell Occupancy, 3DFSC sphericity, and SCF*) across 14 #EMPIAR datasets and synthetic data, providing a comprehensive evaluation of their strengths and applications.

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Structura Biotechnology @structurabio.bsky.social · 17/08/2026

🚀 Preprint alert: cFAR and Relative Signal: Diagnosing Preferred Orientation in Single-Particle #CryoEM Explore how cFAR, tFAR, and Relative Signal provide complementary diagnostics for preferred orientation, tested across 14 #EMPIAR datasets and compared with established metrics 👉 bit.ly/4wBY1Ev

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Structura Biotechnology @structurabio.bsky.social · 13/08/2026

🔄 Mixing Two Cryo-EM Datasets on Purpose: A Cryo-EM Heterogeneity Challenge What happens when you deliberately mix two #cryo-EM datasets containing different conformational states? Our latest #CryoSPARC blog distills the practical lessons from the accompanying FaNaC1 case study 👉 bit.ly/3ScIZa3

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Structura Biotechnology @structurabio.bsky.social · 10/08/2026

🇨🇦 This week we’re at IUCr 2026 in Calgary! Join us during Poster Session 4B (Sunday 15th, 5-6 PM MDT), at poster #486, where we’ll be presenting: 🔹 Updates and upcoming developments in #CryoSPARC 🔹 Advances in automated #cryo-EM data processing 👉 www.iucr2026.org

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Structura Biotechnology @structurabio.bsky.social · 03/08/2026

✨ We’re at the Microscopy & Microanalysis conference in Milwaukee (WI) this week! Join us during session B04.2, where we’ll be showcasing recent #CryoSPARC developments and discussing the latest advances in single-particle #cryoEM data processing and analysis. 👉 mmconference.microscopy.org

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Structura Biotechnology @structurabio.bsky.social · 30/07/2026

🔬 Cryo-EM Reveals the Molecular Machinery of Tubulin Assembly In a recent study from @ucdavis.bsky.social, #cryoEM and #CryoSPARC were used to uncover the molecular mechanism underlying α/β-tubulin biogenesis! Read more on the latest CyoSPARC blog 👉 cryosparc.com/blog/tubulin

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Structura Biotechnology @structurabio.bsky.social · 27/07/2026

✨ We’ll be at the Structure-Based Drug Design Summit in Boston this week, showcasing recent #CryoSPARC developments for industrial applications in #cryoEM and #SBDD. If you're attending, we'd love to connect! structure-based-drug-design-summit.com

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Structura Biotechnology @structurabio.bsky.social · 23/07/2026

🚀 Can automation solve preferred orientation in your #cryoEM datasets? After automating processing across 21 GPCR datasets in #CryoSPARC, we updated the workflow to be even more robust to preferred orientation, achieving improved map quality over manual processing. Read more 👉 bit.ly/3RyOIGG

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Structura Biotechnology @structurabio.bsky.social · 21/07/2026

This first post is inspired by our recent case study on acetogenin-bound complex I (EMPIAR-10927), where refinements using 90,066 particles and only 19,090 particles produced comparable map quality and nominal resolution. Explore why!

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Structura Biotechnology @structurabio.bsky.social · 21/07/2026

Digging Deeper complements our technical case studies by unpacking the methods behind the results. Rather than diving into implementation details, these posts explain the concepts and practical choices that matter during #cryo-EM data processing.

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Structura Biotechnology @structurabio.bsky.social · 21/07/2026

🧩 Why can two refinements with very different particle counts produce nearly identical maps? Our new “Digging Deeper” series breaks down #CryoSPARC case studies in a practical way, starting with how refinement parameters shape reconstruction quality. Read more 👉 cryosparc.com/blog/paramet...

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Structura Biotechnology @structurabio.bsky.social · 16/07/2026

Finally, new pages have been added to the Cryo-EM Foundations section. 🔹Fourier Shell Correlation and Gold-Standard Refinement guide.cryosparc.com/the-fsc-and-... 🔹Symmetry in CryoSPARC guide.cryosparc.com/symmetry-in-...

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Structura Biotechnology @structurabio.bsky.social · 16/07/2026

Our expanded 3D Refinement overview now provides a deeper look at the principles behind #cryo-EM refinement: 🔹3D Refinement guide.cryosparc.com/processing-d...

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Structura Biotechnology @structurabio.bsky.social · 16/07/2026

Explore these rewritten pages to learn about the most commonly adjusted parameters in Refinement and Reconstruction jobs: 🔹Homogeneous Refinement bit.ly/4w57kgy 🔹Non-Uniform Refinement bit.ly/4w57qEW 🔹Homogeneous Reconstruction Only bit.ly/4fyslKN 🔹Heterogeneous Reconstruction Only bit.ly/4hgjezz

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Structura Biotechnology @structurabio.bsky.social · 16/07/2026

🚀 New and expanded #CryoSPARC Guide pages are here! 📚 We present to you several new and updated pages, featuring new figures and explanations, along with learning materials designed for both new and advanced #cryoEM practitioners. Brief summary of what’s new in the thread!

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Structura Biotechnology @structurabio.bsky.social · 14/07/2026

✉️ The latest #CryoSPARC Newsletter is out! Highlights include: 🔹 Sept 2026 workshop hosted with SickKids 🇨🇦 🔹 New cryosparc-tools video 🔹 New case study on discrete heterogeneity 🔹 Small-protein #cryoEM advances 🔹 Dynamic masking guide (v5) Read more here: mailchi.mp/b8fb173dac5c...

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Structura Biotechnology @structurabio.bsky.social · 06/07/2026

🇨🇦 The Toronto-based #CryoSPARC Workshop in collaboration with SickKids is back this September! Dive deep into single particle data processing with the experts at Structura. 🗓️ When | Sept 24-25, 2026 📍Where | SickKids Patient Support Centre, Toronto Info and registration: cvent.me/dL1wem #cryoEM

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Structura Biotechnology @structurabio.bsky.social · 30/06/2026

☀️ Last week we had the pleasure of contributing to the UCSC #CryoEM Workshop in Santa Cruz with a hands-on #CryoSPARC data processing session. ✨ Thank you to the organizers and everyone who participated. We look forward to many more opportunities to contribute to workshops like this!

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Structura Biotechnology @structurabio.bsky.social · 23/06/2026

☀️ Structura will be at the UCSC CryoEM Workshop in Santa Cruz this week, leading a hands-on workshop on #cryoEM #SingleParticleAnalysis with #CryoSPARC. Looking forward to exchanging ideas and strategies, and chatting about all things cryo-EM! See you there!🔬 👉 sites.google.com/view/ucsccry...

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Structura Biotechnology @structurabio.bsky.social · 27/05/2026

🚀 We're excited to announce the stable release of CryoSPARC v5! After a successful public beta and months of testing, we’re excited to share this major milestone, made possible by feedback from the amazing #CryoSPARC community. Learn more 👉 cryosparc.com/blog/v5-0-st... #cryoEM #StructuralBiology

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Structura Biotechnology @structurabio.bsky.social · 25/05/2026

🔬 We’ll be at the 6th NY Area #CryoEM Meeting on May 27, presenting recent developments in #CryoSPARC for Homogeneous Ab Initio Refinement and workflows for small proteins. bit.ly/4dYMUze We’re also proud to support the meeting as a sponsor — stop by our poster to learn more!

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Structura Biotechnology @structurabio.bsky.social · 15/05/2026

What an incredible #Cryo-EM SPA mini-course last week, hosted by @royalmicrosoc.bsky.social & ThermoFisher Scientific! 🚀 Huge thanks to all the participants who joined the webinar, and to the experts who shared their insights on #SingleParticle #cryoEM workflows.

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Structura Biotechnology @structurabio.bsky.social · 07/05/2026

We will be at the @proterosma.bsky.social IBA Conference in Boston next week! Stop by our poster to chat about the latest developments in #CryoSPARC, including new results from fully automated data processing for #sbdd repeat-target scenarios & v5 features 🔬 bit.ly/49hQoKo #SingleParticle #Cryo-EM

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Structura Biotechnology @structurabio.bsky.social · 23/04/2026

We are at the CCP-EM Spring Symposium XII in Nottingham! Come check out our talk (Session 6, 11:00) and Poster 12 (Session 1) to stay updated on new developments for #SingleParticle #Cryo-EM Data Processing in #CryoSPARC!

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Structura Biotechnology @structurabio.bsky.social · 30/01/2026

Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

11/ And finally, v5 connects the #CryoSPARC app with all of the detailed documentation (guide.cryosparc.com) that our team produces, with embedded docs links throughout the interface!

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

10/ Resource usage (GPU, CPU, and RAM) is now clearly visible across the interface, making it easier to diagnose bottlenecks, optimize scheduling and make better use of shared compute resources.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

9/ Additional workflow improvements for #cryo-EM: Tags can now be created inline and applied to multiple jobs at once, parameters can be copied + pasted across jobs, and job info tags (job card stats) can be exported.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

8/ Uploading external 3D volumes and masks into CryoSPARC is smoother. When Auto-Import is enabled, CryoSPARC will automatically create and queue an Import 3D Volumes job when .mrc or .map files are uploaded.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

7/ In the tree view, upstream jobs are now collapsed into grouped cards, making it easy to stay focused on the jobs relevant to your workspace, and see where jobs from other workspaces are connected.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

6/ The Job Dashboard brings all key outputs, plots, and diagnostics into one place. Interpret results at a glance and see changes over iterations. With the multi-job Comparison View, immediately spot differences and make decisions about the effect of a parameter or input change.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

5/ v5 updates to CryoSPARC Live in make real-time processing easier under real-world data collection and hardware constraints. Delay the start of a Live session until new data arrives, auto pause when no new data has been found, and run multiple preprocessing workers per GPU.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

4/ Homogeneous Ab-Initio Refinement enables refinement to high resolution from scratch, while preserving gold standard independence. Inspired by @olibclarke.bsky.social group’s results showing that Ab-Initio Reconstruction can improve map quality for small challenging targets.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

3/ 3D refinements now perform per-particle scale optimization by default. The optimized values measure particle quality, used during reconstruction to down-weight low quality particles. The values can also be useful for separating low-quality particles from a dataset.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

2/ Masking during 3D refinement has been significantly reworked. Dynamic mask generation now adapts automatically as resolution changes, reducing the likelihood of overfitting or overestimated resolution, without users needing to modify parameters.

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Structura Biotechnology @structurabio.bsky.social · 27/01/2026

🚀 CryoSPARC v5.0 BETA is here! We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread! Full changelog: cryosparc.com/updates/v5.0.0

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Structura Biotechnology @structurabio.bsky.social · 30/10/2025

On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look? Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner! guide.cryosparc.com/processing-d...

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Structura Biotechnology @structurabio.bsky.social · 20/10/2025

The workflow is powered by new methods, including micrograph junk detection, denoising, and reference based auto selection of 2D classes and 3D volumes. Read the preprint for more insights and details.

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Structura Biotechnology @structurabio.bsky.social · 20/10/2025

The automated workflow generalizes easily to new target classes. We provide downloadable #CryoSPARC Workflow JSON files so that users can replicate, adapt and extend it for their own targets. All required jobs and tools are already available in v4.7.1+.

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Structura Biotechnology @structurabio.bsky.social · 20/10/2025

In 10 of 21 cases, the automated results enable improved model building of the ligand, binding pocket, side chain positioning and/or extracellular loops. Three striking examples are shown here and in the first video.

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Structura Biotechnology @structurabio.bsky.social · 20/10/2025

We test across 21 challenging active and inactive GPCR datasets. Despite small particle size and the presence of contaminants, empty micelles, broken, denatured and aggregated particles, our automated workflow yields equal or better resolution and map quality in 17 of 21 cases.

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