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Benjamin Ambrose

@single-mole.bsky.social

671 followers 515 following 32 posts

Single-molecule Biophysicist at London Institute of Medical Sciences 🧬🔬✨ My research interests are all things single-molecule fluorescence and nucleic acids. Other hobbies: archery, chess, sci-fi

Posts Replies Media

Benjamin Ambrose @single-mole.bsky.social · 01/07/2026

The program can currently be accessed in browser at - singlemoleculegroup.github.io/kymosketch-a..., no install required. Just drag and drop your kymos in or use the demo data already there if you want to try. (6/6)

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Benjamin Ambrose @single-mole.bsky.social · 01/07/2026

I’ve also added fluorescence intensity analysis, and FRET. I will be adding FRET correction parameters soon, for those who like their FRET efficiencies precise and accurate. (5/6)

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Benjamin Ambrose @single-mole.bsky.social · 01/07/2026

Once you have your tracks, you can do diffusion analysis - whole trace MSD, rolling diffusion, or DeepDiffusion - and the results of these can be viewed globally for all tracks, including off-rate analysis taken from trajectory length (4/6)

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Benjamin Ambrose @single-mole.bsky.social · 01/07/2026

You can also very easily change colours in kymolab, if you want something more colourblind friendly than RGB. If displaying only one colour you can also use perceptually uniform colourmaps like Viridis (3/6)

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Benjamin Ambrose @single-mole.bsky.social · 01/07/2026

It’s split into two halves, kymolab and tracklab. In kymolab you load .h5 filess (from c-trap systems) or TIFF files (from anywhere). You generate sketches (candidate tracks) using a trackspotter or manual drawing, then refine them into finalised tracks by fitting. (2/6)