iiinova — where your digital self keeps evolving

Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 23/04/2026

🚨📢 New paper online 🚨📢 Four Xlinking flavors in living cells, up to 1000-fold faster. Highlights include Xlinking kinetics of the RNA-binding proteome and a new way to quantify cytosolic RNA interactions only minutes after ribotoxic stress. #RNASky #Chromatin #Proteomics doi.org/10.1093/nar/...

doi.org

Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes

Abstract. The activation of chemical reactions in living cells using ultraviolet (UV) light enables the interrogation of biomolecules in their native envir

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Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 20/11/2025

New preprint 🚨 We systematically measured 17 million phospho-specific dose-response curves (133 kinase inhibitors × 5 cell lines) to decrypt the kinases that shape the human phosphoproteome. We show that drug perturbation potency (not effect size) links kinases to substrates while controlling FDR.

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Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 09/11/2025

Hello Toronto!🍁The Terrific TUM Team is happy to be attending #HUPO2025. Kusterlab, Wilhelmlab and Leelab have a diverse set of presentations for you that we can't wait to share 🤓. We're excited to spend the next days reuniting with old friends and making new connections. See you there!

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Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 09/09/2025

New preprint: We isolate peptide–RNA photo-crosslinks with tunable RNA chains from living cells for mass spec. This maps over 4,700 crosslinking sites across 744 proteins and offers the first glimpse into the RNA sequences in crosslinks by MS. Read here: doi.org/10.1101/2025...

doi.org

Peptide-RNA photo-crosslinks with tunable RNA chain map protein-RNA interfaces

Photo-crosslinking mass spectrometry enables the identification of protein-RNA interactions in living cells, pinpointing interaction interfaces at single-amino acid resolution. However, current isolat...

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Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 25/08/2025

New Preprint: High-intensity 365 nm irradiation accelerates photoreactions in living cells by up to 1000×. We show protein–drug 💊, –protein 💪, –DNA 🧬, –RNA 🧬 crosslinking within seconds, and analysis by #massspec for #proteomics, #chembio, #chromatin and #RNAbiology. www.biorxiv.org/content/10.1...

biorxiv.org

Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light

The activation of chemical reactions in living cells using ultraviolet (UV) light enables the interrogation of biomolecules in their native environment with photoreactive probes or crosslinking reagen...

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Reposted by Shuyao Sha

Jakob Trendel @jtrend.bsky.social · 23/05/2025

Excited our paper is out in Cell @cp-cell.bsky.social! 🧬⚡ DNA photo-crosslinking proteomics in living cells 🎯 Pinpoints protein-DNA interactions to single amino acids 🌎 Globally quantifies DNA binding for >1800 proteins at a timescale of minutes 🔗 www.cell.com/cell/fulltex... 🧵

cell.com

The human proteome with direct physical access to DNA

Zero-distance photo-crosslinking reveals direct protein-DNA interactions in living cells, enabling quantitative analysis of the DNA-interacting proteome on a timescale of minutes with single-amino-aci...

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Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 22/05/2025

🚨Our new paper is online🚨 We use zero-distance⚡photo-crosslinking⚡to reveal direct protein-DNA interactions in living cells, enabling quantitative analysis of the DNA-interacting proteome on a timescale of minutes. #DNA #Chromatin #Proteomics www.cell.com/cell/fulltex...

cell.com

The human proteome with direct physical access to DNA

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Reposted by Shuyao Sha

Kusterlab @kusterlab.bsky.social · 09/05/2025

New paper! Our chemoproteomic survey of phenylhydroxamic acids identifies the first drug-like inhibitors for nucleotide-binding protein HINT1 and nucleoside kinases NME1-4. On top, we found probes for the HDAC inhibitor off-target MBLAC2. Check out the open-access article! tinyurl.com/yh92vh6b

tinyurl.com

Serendipitous and Systematic Chemoproteomic Discovery of MBLAC2, HINT1, and NME1-4 Inhibitors from Histone Deacetylase-Targeting Pharmacophores

Metalloenzyme inhibitors often incorporate a hydroxamic acid moiety to bind the bivalent metal ion cofactor within the enzyme’s active site. Recently, inhibitors of Zn2+-dependent histone deacetylases...

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