Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins...

Summary: Image analysis pipeline for 3D nuclear instance segmentation and lineage tracking for preimplantation mouse embryos, including description of new H2B-miRFP720 reporter line and a large ground...

Fluorescence lifetime imaging microscopy can offer insights into biological processes such as metabolic imaging, protein–protein interactions and live-cell intracellular dynamics. In this Primer, Torr...