The Crabtree effect affects yeast-based bioproduction. Here, the authors report the development of a Crabtree-negative yeast through introducing the sucrose phosphorolysis pathway, deletion of the pho...

Detecting small molecules is pivotal across many fields. Here, authors leverage a split crRNA mode to construct spatially blocked split CRISPR-Cas12a system. This approach establishes a low-background...

Current base editors cannot simultaneously edit multiple loci with base-pair level precision, hindering complex genotype generation. Here the authors describe optimized Cas12a gRNA array designs, enab...

Prime editors are restricted to performing precise edits downstream of cleavage sites. Here, authors develop helicase-assisted circular RNA-mediated inverse PEs (ciPEs) to increase editing efficiencie...

Expanding editing scope while reducing byproducts is now possible for prime editing. Here, authors develop reverse prime editing (rPE), a SpCas9-based tool enabling editing in reverse window with high...

Engineering cell factories that support the production of large quantities of protein therapeutics remains a significant biomanufacturing challenge. Here the authors engineered cells to sense an early...

Designing high performance RNA molecules is a unifying challenge across many areas of biotechnology research. Here, authors develop GARDN and SANDSTORM, a data-efficient generative AI framework for de...

Directed evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Here the authors develop a directed evolution platform (PROTein Evolution Usin...

Membrane proteins are notoriously difficult to synthesize. Here, authors introduce MEMPLEX, a high-throughput experimentation platform guided by machine learning that designs artificial cell-free envi...

Artificial transcription factors (ATFs) are core elements of customized circuits responsible for signal output and event transformation. Here, the authors report the design of a protease-responsive co...

Genetic code expansion (GCE) enables the incorporation of new amino acids into proteins. Here, authors demonstrate the rapid OrthoRep-driven evolution of aminoacyl-tRNA synthetases for GCE, increasing...

The ribosome has evolved to link standard amino acids into proteins via peptide bonds. Here, authors expand its chemistry to directly synthesize novel 5- and 6- membered cyclic backbones from noncanon...

Genetic variance is vital for breeding programs and mutant screening, yet traditional mutagenesis methods wrestle with genetic redundancy and a lack of specificity in gene targeting. Here the authors ...

Prime editing can generate undesired editing outcomes. Here, authors describe a method for reducing both on-target and off-target imprecise editing outcomes of diverse prime editing modalities in diff...

Single-cell RNAseq can struggle to capture cellular heterogeneity due to the relatively low expression of biologically meaningful transcripts. Here the authors present an approach called scCLEAN, whic...

Cytosine base editors use an uracil-containing intermediate to introduce C•G to T•A and C•G to G•C point mutations. Here, the authors couple gene knockdown with reporters of these two cytosine base ed...

Polyamines are essential metabolites linked to aging, cancer, and Parkinson’s disease. Here, authors develop a live-cell polyamine reporter and use a genome-wide CRISPR screen to uncover a link betwee...

Mitochondria play a key role in cellular metabolism. Here, authors develop a Variational Autoencoder to design novel mitochondrial targeting sequences, validating them across several eukaryotic organi...

Upcycling urine in wastewater for nitrogen and phosphorus production has gained attention, but their low market values hamper the application. Here, the authors develop a yeast platform that mimics os...

Carbon monoxide (CO) present in syngas severely inhibits growth of many acetogens. Here, the authors adapt, characterize, and engineer utilization of CO as sole carbon and energy source by the thermop...

The authors demonstrate the role and mechanism of triacylglycerol (TAG) cycling in polyunsaturated fatty acid biosynthesis and develop a strategy of decoupling the TAG biosynthesis and degradation to ...

The position of hydroxyl groups, the carbon number of the alkyl chain, and the modification of the side chain are key factors determining the function of diols. Here, the authors report a biosynthetic...

Here, the authors show that a modular deregulation strategy in Saccharomyces cerevisiae boosts xylose conversion to 3-HP by engineering central carbon metabolism with five strategies, achieving a 4.7-...

Synthesis of complex molecules requires reactions from multiple pathways operating in balanced subnetworks. Here, the authors report a computational algorithm that combines the strength of constraint-...

Heavy metals and plastics are two major environmental pollutants. Here, the authors engineer microbial platforms capable of endogenously biosynthesizing phosphomelanin for simultaneously heavy metals ...

The implementation of one-carbon (C1) biomanufacturing is limited to laboratory or pilot scales. Here, the authors highlight the economic and technical challenges associated with scaling up C1 biomanu...

Semi-synthesis of artemisinin has been achieved by autooxidation of dihydroartemisinic acid (DHAA), but DHAA supply relies on chemical synthesis from artemisinic acid. Here, authors report the identif...

Bioproduction of steroids involves NADPH-dependent enzymes that mediate electron transfer reactions. Here, the author reveal the electron transfer mechanisms of NADPH-dependent enzymes and demonstrate...

Available enzymatic CO2 reduction strategies are not suitable for aerobic microorganisms and many industrial settings. Here, the authors design a new metabolic pathway that can operate under fully aer...

Identification of genetic determinants is pivotal for improving microbial biosynthesis of desired products. Here, the authors employ genome-scale CRISPRi modulation combining fluorescence-activated ce...

Arrayed and pooled high-throughput screening is crucial for drug discovery and CRISPR functional genomics. Here, the authors present dFLASH; a dual FLuorescent transcription factor Activity Sensor for...

pegRNA scaffold incorporation is a common by-product of prime editing, compromising its precision and safety. Here, the authors show that an internal abasic spacer or 2’-O-methylation within pegRNAs m...

Gene-edited mouse models are crucial for disease research but remain challenging to create. Here, authors introduce the CRISPR-VIM, using virus-like particles to efficiently deliver CRISPR tools into ...

The authors introduce SelectID, an approach combining CRISPR-guided targeting with methylation-sensitive labeling to identify proteins interacting with methylated repetitive sequences. They suggest th...

The flexibility of CRISPR/Cas9 editing is severely constrained by the requirement that the modification site is located in close proximity to the landing sequences. Here, authors develop a universal s...

Genome engineering in bacteria remains a complex multistep process. Here, the authors developed make-or-break Prime Editing, a precise method for genetic modification in Streptococcus pneumoniae that ...

Here, the authors describe a highly efficient gene drive targeting the non-coding miR-184 gene. Disruption of the miR-184 gene by the gene drive reduces mosquito lifespan and interferes with survival ...

Rapid diagnosis of bloodstream infections is vital for timely effective treatment. Here, Mao and Wan et al developed a method using a dual stem-loop DNA signal amplifier, to achieve ultrasensitive, ra...

Authors report a codon language representation and a model, CodonTransformer, open access and with a user-friendly interface. CodonTransformer generates species-specific DNA sequences with natural-lik...

Metabolic interactions shape ecosystems but remain hard to study. Here, authors present MetaFlowTrain, a modular, low-cost fluidic system controlling metabolic fluxes between microbes, and show its va...

Engineering multicellular consortia, where information processing is distributed across specialized cell types, offers a promising strategy for implementing sophisticated biocomputing systems. Here th...

Dysregulated protein degradation drives diseases like cancer. Here, authors use protein language models to design target-binding peptides, which are subsequently attached to the catalytic domain of th...

Synthetic multicellular systems have great potential for performing complex tasks, including multi-signal detection and computation through cell-to-cell communication. Here the authors engineer microb...

Constructing an efficient microbial cell factory still requires exploring and selecting various host strains, as well as identifying the best-suited metabolic engineering strategies, which demand sign...

Biotechnology offers a sustainable route to manufacturing, but closing the loop towards safeguarding biodiversity remains challenging. Here, we explore how partnerships with Indigenous Peoples and Loc...

CRISPR-Cas12a is a programmable endonuclease used for genetic engineering. Here, the authors use real-time single-molecule measurements to show that Cas12a unwinds the target DNA site in dynamic and r...

Selecting the appropriate Cas12a among the many orthologs for a given target sequence remains difficult. Here, Peng Chen et al. evaluate the activity and specificity of Cas12a variants and facilitate ...