Happy to share our recently accepted research article in iScience(Cell Press). Indeed it was an excellent team work. Special thanks to project PI @christianslentz.bsky.social, co-PI @monaj66.bsky.social and excellent CRISPRi collaborator @mkjos.bsky.social. Wish to learn many more with this tool!
cell.com
A programmable, selection-free CRISPR interference system in Staphylococcus aureus for long-term host interaction studies
Common dCas9-based CRISPR interference (CRISPRi) systems for manipulating bacterial gene expression require antibiotic selection and exogenous inducer molecules, limiting their applicability in infection models. For Staphylococcus aureus, we have developed a programmable, selection-free CRISPRi system leveraging the pCM29 plasmid, which is stable without antibiotic selection. In this system, dCas9 expression is regulated by an endogenous promoter, and sgRNA expression is driven by a constitutive promoter, eliminating the need for exogenous inducer molecules.
