Nature Protocols, Published online: 19 March 2026; doi:10.1038/s41596-026-01336-yThis protocol enables users to establish a defined, in vitro consortium composed of seven fully sequenced and anaerobically culturable human gut bacteria and follow the growth of individual members via strain-specific qPCR to evaluate community dynamics in response to interventions.

Nature Protocols - This protocol enables users to establish a defined, in vitro consortium composed of seven fully sequenced and anaerobically culturable human gut bacteria and follow the growth of...