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Protea Glycosciences @proteaglyco.bsky.social · 30/04/2026

Here’s one public aspect of our cutting edge methods for glycan and polysaccharide detection. The result: more confident characterisation leading to more successful projects.

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Protea Glycosciences @proteaglyco.bsky.social · 13/04/2026

Here are the links to the preprint (chemrxiv.org/doi/full/10....) and publication (pubs.acs.org/doi/10.1021/...). We think it's pretty neat so we're happy to discuss methodology or how it fits into existing workflows. More will be coming very soon in this space from us (4/4)

pubs.acs.org

GlyCombo Enables Rapid, Complete Glycan Composition Identification across Diverse Glycomic Sample Types

Glycans are sugar-based polymers found to modify biomolecules, including lipids and proteins, as well as occur unconjugated as free polysaccharides. Due to their ubiquitous cellular presentation, glyc...

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Protea Glycosciences @proteaglyco.bsky.social · 13/04/2026

The result is composition analysis that's faster AND automation-ready out of the box as we are fully compatible with a vendor neutral format (mzML) and results can be directly imported into downstream software (Skyline). (3/4)

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Protea Glycosciences @proteaglyco.bsky.social · 13/04/2026

The core problem: glycomics data analysis is relatively fast initially but slow to integrate. Most tools hand off between steps with no native automation layer. GlyCombo was designed around that gap. (2/4) github.com/Protea-Glyco...

github.com

Release GlyCombo v1.3 · Protea-Glycosciences/GlyCombo

Minor fix to remove and resolve false formula matches when searching permethylation with sulfation (reported by Kat). Native derivatisation searches are unaffected by this change. Changes: Permeth...

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Protea Glycosciences @proteaglyco.bsky.social · 13/04/2026

🧵 We published GlyCombo in 2024 and it's still one of my favourite things we've built. Here's what it actually does and why it matters for glycomics labs. (1/4) #glycotime

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Reposted by Protea Glycosciences

Chris Ashwood @cashwood.proteaglyco.com · 22/12/2025

Closing out the year at @proteaglyco.bsky.social with real momentum. We advanced native glycomics to reveal often-missed labile modifications, and saw global uptake of our GlyCombo software. Huge thanks to our team and collaborators, building towards an even more accelerated, high-impact 2026.

A man sitting at a liquid chromatograph and mass spectrometer used for glycan analaysis.

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Reposted by Protea Glycosciences

Chris Ashwood @cashwood.proteaglyco.com · 05/02/2026

On my way to Lorne, Australia for #LorneProteomics2026. Looking forward to seeing the latest proteomics and glycomics advances. I’ll be presenting our glycomics platform that more truthfully describes the glycome resulting in greater glycopeptide identifications. Happy to meet up if you’re there!

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Protea Glycosciences @proteaglyco.bsky.social · 20/10/2025

Finally, we leveraged all of this to see if O-acetylation was present on human N-glycans from serum. Specifically, we had O-acetylation on NeuGc in mice, and on NeuAc in rats, so the combined approach should uncover if it is present in humans Short answer: trace amounts in human at best. #glycotime

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Protea Glycosciences @proteaglyco.bsky.social · 20/10/2025

We then developed a quality control workflow, using as much information as possible to confirm O-acetylation. Using MS1, MS2, and LC, we reduced the number of putative identifications by 95%, ensuring the quality of these annotations.

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Protea Glycosciences @proteaglyco.bsky.social · 20/10/2025

Second level, liquid chromatography: - O-acetylated structures elute later - Their peak shape is broader We're not quite sure why we have broader peak shapes, but it was universal. Further work is ongoing to resolve this, but it could be caused by multiple acetylation positions (more structures!).

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Protea Glycosciences @proteaglyco.bsky.social · 20/10/2025

Now we're seeing these structures, we want to characterise them for community use, specifically, by LC-MS/MS. First fragmentation: - Beam-type (HCD) CID is best, as it generates universally useful fragments for all acetylated structures - Energy profiles show the expected ratios of these fragments

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Protea Glycosciences @proteaglyco.bsky.social · 20/10/2025

Traditionally, reduction or permethylation has been optimal to measure glycan structures, but we've previously reported a way to avoid this (link: www.biorxiv.org/content/10.1...). By performing native glycan analysis, we are suddenly seeing 2x the structures previously observed in rats and mice.

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Protea Glycosciences @proteaglyco.bsky.social · 20/10/2025

Keen to share our latest work where we apply our native PGC-LC-MS glycan analysis workflow and see what is missed with traditional glycomics workflows (reduction or permethylation) Here, we found intact O-acetylation of sialic acids on N-glycans in mouse and rat sera www.biorxiv.org/content/10.1...

biorxiv.org

Native PGC-LC-MS Profiling Reveals Distinct O-Acetylation Patterns of Sialylated N-Glycans Across Mammalian Sera

O-acetylation of sialic acids represents an additional layer of structural diversity and biological complexity, occurring at various hydroxyl positions (commonly C-7, C-8, or C-9) of the sialic acid r...

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Protea Glycosciences @proteaglyco.bsky.social · 23/05/2025

#ausomics was a great opportunity for Protea Glycosciences to share our recent advances in Glycomics and glycoproteomics and see the latest research across four Australian societies including @ozglyco.bsky.social. It was a productive conference and now we're looking forward to getting back to work.

Chris Ashwood presenting a glycoproteomics platform that is isomer-focused.

Maia Kelly presenting GlyComboCLI, a bioinformatic tool for matching glycan compositions to MS2

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Protea Glycosciences @proteaglyco.bsky.social · 01/04/2025

Proud to announce our business relocation, our local beach. We’re committed to accelerating glycoscience, so where better to be based than a location where the glycans come to us. Our LC-MS service engineers are excited for the opportunities provided by this new location.

Protea Glycosciences new business location, a beach full of seaweed.

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Reposted by Protea Glycosciences

Chris Ashwood @cashwood.proteaglyco.com · 31/03/2025

Excited to be presenting @proteaglyco.bsky.social's glycoproteomics workflow at Aus-Omics on Monday at the New Technologies session. We're bringing structure back to how we identify and report glycopeptides, plus we'll describe how our unique approach to glycan structure excels for glycopeptides.

AUS-oMicS May 18-25, 2025.
Abstract title: Resolving glycopeptide structural ambiguity: A new MS workflow enables definitive glycoprotein characterization

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Protea Glycosciences @proteaglyco.bsky.social · 24/03/2025

We're just completing an R&D sprint to identify an LC additive that increases our glycan identifications 3x and enhances glycan charge states by up to 45%🚀. It's too early to publicly disclose its identity, but here's a results preview. Interested? Message or email us, we'd love to collaborate.

Manuscript figure describing our method for evaluating mobile phase additives for enhanced negative mode LC-MS of glycans. The results show different O-glycan compositions having up to 40% higher charge states.
Interested in how our platform can help you discover more from your samples? Email us at info@proteaglyco.com to collaborate.

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Protea Glycosciences @proteaglyco.bsky.social · 03/02/2025

Our pure glycan structures provide essential tools for researchers in glycomics, diagnostics, and therapeutic development. Partner with us to accelerate your glycan-based discoveries! #Glycobiology #TeamMassSpec #Biopharma #Diagnostics (P.S. NeuGc structures coming soon!) proteaglyco.com/shop/

proteaglyco.com

Shop - Protea Glycosciences

We are a glycoscience company established in 2023 with the aim of accelerating glycoscience research.

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Protea Glycosciences @proteaglyco.bsky.social · 24/01/2025

Want to work with mass-based glycan data? Try out GlyCombo, our published, freely available software that rapidly assigns monosaccharide combinations to glycan masses. Here's a 5 min video showing you how to get started. Publication: pubs.acs.org/doi/10.1021/... Download: github.com/Protea-Glyco...

youtube.com

GlyCombo Quick Start Tutorial

YouTube video by Protea Glycosciences

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