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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 05/10/2026

🔵⚪🔴 - Vous avez raté l'émission sur la phagothérapie avec Laurent Debarbieux ce samedi 3 octobre ? Pas de panique! Vous pouvez aller la ré-écouter: Radio France www.radiofrance.fr/franceinter/... Apple podcasts podcasts.apple.com/fr/podcast/b... Spotify open.spotify.com/episode/2JGL...

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 05/10/2026

Were you not able to make it to this on Saturday? We got you covered! You can listen to The Boss talking about #phage therapy on multiple platforms: Radio France www.radiofrance.fr/franceinter/... Apple podcasts podcasts.apple.com/fr/podcast/b... Spotify open.spotify.com/episode/2JGL...

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 03/10/2026

The Boss is in Radio France ......... NOW!!!! You should tune in 📻 www.radiofrance.fr

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 28/09/2026

We believe this face will be familiar to the #phage community in here The BBH lab is quite pleased to be hosting Jeremy Barr (@jeremyjbarr.bsky.social), from Monash University, for the upcoming months. We were off to a good start with his presentation on gut #microbiome last week @pasteur.fr

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 18/09/2026

2026 seemed to be the summer of #phage in the press 🎥 and we've been happy to participate This is a good way to get the message out there about phage #therapy and we're definitely noticing increased interest The Boss is scheduled for a couple more of these interviews. We'll keep you posted 😎

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 15/09/2026

New lab member at the BBH!! 🚨 Niamh Diver-Hall from the @uofgsii.bsky.social is joining our #gut team for the next year, as part of her Master's degree Her work will center in #phage defense and antidefense systems in gut bacteria 🦠 Welcome!!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 09/09/2026

The Boss was invited to participate in the #ERSCongress2026 🫁 congress this week in Barcelona He got to advocate for #phage therapy in front of a pretty sizeable crowd during the "Beyond antibiotics" session Thanks for the invite!! @europeanrespsoc.bsky.social

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 31/07/2026

Thanks to the folks at KU Leuven, particularly @hannelorelongin.bsky.social , for welcoming @moreautanais.bsky.social , one of our PhD candidates, this week! Tanaïs was happy to discuss all things "phageFACTor" but there was also time to enjoy some local views ...... and delicacies 🍟🍪

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 10/07/2026

THAT'S A WRAP ON #VoM2026 FOR THE BBH LAB !!! We had a blast sharing all things #phage with all the teams present A HUGE congrats to the organizing committee for their amazing work all these days, they really knocked it out of the park #phagesky

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 10/07/2026

The Viruses of Microbes conference is coming back to France!!! 🇫🇷 We're also happy to share that The Boss will be part of the organizing committee, led by the great team of Fréderic Laurent See you in Lyon in 2028!!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 10/07/2026

Well, the audience responded with something else, as well Alejandro's presentation was awarded the Intralytix award for "Best oral presentation" in the phage therapy category 🥳 A nice capper, recognizing the work of the BBH lab at #VoM2026

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 09/07/2026

We were very happy to show some of our Immunophage synergy work at #VoM2026 during the Phage therapy session Our Postdoc @bohorquezalejo.bsky.social was able to summarize our work on this area and the audience responded with multiple, insightful questions

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 08/07/2026

If you're in the mood for some (un)friendly bacterial competition, Nandita Sharma can tell you all about it and how prophages are involved 🦠 We keep hearing that prophages are cool, we'll keep you posted in any developments 😎 #phagesky #VoM2026

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 08/07/2026

If immune response to phages is more of your cup of tea, we got you covered! Nicolas Dufour will be at poster number 93 to talk about phage-induced antibody response 🩸 #phagesky #VoM2026

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 08/07/2026

Getting an early start for today's poster session at #VoM2026 First up, we have poster 17 by @ltanoeiro.bsky.social If you're interested in prophages or Campylobacter, you should come talk to him

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 07/07/2026

On the same session, Marie Messika will be discussing her recent results on enteric phages at board 132 You don't wanna miss it #phagesky #VoM2026

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 07/07/2026

If you happen to be at #VoM2026 the BBH lab has content for you on EVERY poster session 😎 On Tuesday, you can come by board 225 to check out Solène's work on tail fiber replacement See you at 2:30!!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 06/07/2026

The BBH is ready for #VoM2026 !!!! 🦠 We've been roaming the sites and taking in beautiful Prague The conference is off to a very good start with today's lectures + welcome reception We'll try to keep you updated on the proceedings

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 01/07/2026

We’re happy to introduce phageFACTor (Functional Annotation and Curation Tool) 👩‍💻Developed by Tanaïs Moreau, our most recent PhD candidate, and building on Phold, we were able to improve annotation of prophage genes by as much as 50% 🧬 You should check it out github.com/TMs-code/pha...

In this example, we're showing the improvement in the percentage of annotated genes from prophage "Maintenon" from Clostridium innocuum (doi: 10.1186/s40168-023-01541-x) using the phageFACTor pipeline

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 08/06/2026

Filipa Vale (IMED) and Alexander Harms (@ethz.ch) came by last week and we had some great discussions on all things #prophage and #phage It's not every week you have not one but TWO great scientists visiting you, so we had to celebrate with a nice apéro Hope to have you guys here more often!!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 03/06/2026

We took a road trip yesterday to @inrae-france.bsky.social and had a pretty fun time sharing with our collaborators over there Despite having worked together for a while, some of us couldn't put faces to names over there, so it was a great opportunity to share with them Thanks for hosting us!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 26/05/2026

❓- Immunological response to the phages was puzzling, since there seems to be a lack of anti-phage IgG in the patient, despite long-term exposure Why isn't there class switching in the host? Is cellular immunity reducing their activity?

Fig. 5: Intravenous phage efficacies are differentially tempered by antibody-mediated neutralization.

Taken from Luong, T., Kharrat, L., Champagne-Jorgensen, K. et al. Ecological partitioning enables phage–antibiotic cooperation in a human Pseudomonas infection. Nat Commun 17, 2615 (2026). https://doi.org/10.1038/s41467-026-69247-w

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 26/05/2026

✅ - This allowed for a peculiar scenario in which a bacterial population could be evaluated for a long period of time in a host environment The authors use a multidisciplinary approach that favours the interpretation of the bacteriological findings on a clinical context

Fig. 2: Co-therapy accelerates airway obstruction relief across functional and radiographic measures.
a, b Spirometric evidence of improved airway mechanics. a Forced vital capacity (FVC, green) and percent-predicted FVC (ppFVC, blue) over time. Dashed line indicates mean baseline FVC = 3.00 ± 0.12 L. b FEV₁/FVC ratio, representing the relationship between airflow and volume, expressed as percent change over time. The declining ratio during treatment reflects persistent small-airway damage limiting full recovery. c Quantitative computed tomography (qCT) chest images in the axial lung window after 2 days of ciprofloxacin alone (left) and one day post-coadministration (right). Reduced opacity in the left upper lobe indicates decreased inflammation. d Bar graphs showing reduced lobar air-trapping percentages following co-therapy. Values are reported for total lung (TL), right lung (RL), left lung (LL), right upper lobe (RUL), right lower lobe (RLL), left upper lobe (LUL), and left lower lobe (LLL). e Mid-maximal expiratory flow (MMEF, L s−1) derived from spirometry confirms partial small-airway clearance. f Principal-component analysis (PCA) biplot of 16 dimensionally reduced patient data sets integrating pulmonary, hematologic, and renal indices. Each circle represents a single day’s standardized data, colored by treatment phase; hierarchically defined clusters are circled. Arrows indicate variable loadings (see Table S5). Vector directionality and sample convergence toward baseline indicate an additive contribution of phage therapy relative to antibiotic monotherapy.

Taken from Luong, T., Kharrat, L., Champagne-Jorgensen, K. et al. Ecological partitioning enables phage–antibiotic cooperation in a human Pseudomonas infection. Nat Commun 17, 2615 (2026). https://doi.org/10.1038/s41467-026-69247-w

Fig. 3: Distinct phage and antibiotic timescales collapse multidrug-resistant Pseudomonas and restore a lower-burden chronic state.
a–c Quantification of bacterial dynamics in sputum. a Relative abundance of Pseudomonas in metagenomes. b Absolute abundance normalized to human reads, revealing bacterial load reductions otherwise obscured by dominance. c Layered area chart of mucoid (blue) and nonmucoid (yellow) subpopulations, expressed as colony-forming units (CFU) per mL of sputum. Persistent layering of mucoid behind nonmucoid counts indicates phenotype dominance. Vertical dashed lines mark key events: ciprofloxacin initiation (day –4), phage initiation (day 0), and end of co-therapy (day 14). d Heatmap of isolate susceptibilities to phages P1A, PYO2, and ciprofloxacin (CIP), separated by phenotype and collection day. Plaque formation indicates sensitivity (yellow); absence indicates resistance (maroon). Asterisks denote isolates analyzed by automated antibiotic susceptibility testing

Taken from Luong, T., Kharrat, L., Champagne-Jorgensen, K. et al. Ecological partitioning enables phage–antibiotic cooperation in a human Pseudomonas infection. Nat Commun 17, 2615 (2026). https://doi.org/10.1038/s41467-026-69247-w

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 26/05/2026

📝 - Authors use the case of a cystic fibrosis patient infected with P. aeruginosa and treated with a combination of antibiotics + phage to explore chemobiotherapy, a concept previously used in the cancer field (with some differences) This entailed the evaluation of the patient for >100 days !!!

Figure 1. a Timeline of clinical events (gray) and intravenous (IV) antimicrobial treatments (colored) for pulmonary exacerbation in an elderly patient with CF. Time is shown relative to the first IV phage dose (vertical dashed line, day 0); the second dashed line marks the end of all IV therapies (day 15). First-line colistin + ceftazidime was discontinued early due to acute kidney injury (AKI). b Posterior-to-anterior chest x-rays at hospitalization (left) and immediately before co-therapy (right) show persistent left-upper-lobe consolidation (yellow arrow). The reduced but unresolved opacity underscores the need for continued treatment. c Heatmap of minimum inhibitory concentrations (MICs) for patient sputum isolates (values in Table S2). Mucoid colonies (top) were largely susceptible, whereas nonmucoid colonies (bottom) were extensively drug-resistant. ND indicates MIC not determined d Forced expiratory volume in 1 s (FEV₁, green) and percent-predicted FEV₁ (ppFEV₁, blue) over time. The dashed line denotes the mean baseline FEV₁ (1.49 ± 0.06 L).

Taken from Luong, T., Kharrat, L., Champagne-Jorgensen, K. et al. Ecological partitioning enables phage–antibiotic cooperation in a human Pseudomonas infection. Nat Commun 17, 2615 (2026). https://doi.org/10.1038/s41467-026-69247-w

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 25/04/2026

#OTD in #Phage history: In 1873, the phage therapy pioneer Félix d’Hérelle was born!!! His amazing work at @pasteur.fr and other institutions laid the foundation in which groups like the BBH were founded You can read more about his fascinating life story here www.pasteur.fr/en/institut-...

F´elix d’H´erelle, 1873–1949. Contributor: HistoricImages / Alamy Stock Photo

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 26/03/2026

❓ - The authors showed a decreased adsorption of phages in bacteria that are not actively growing. What does this imply for phage replication in the environment where bacteria are not actively replicating ?

Figure 2. Comparing the metabolic condition effect on phage adsorption rate.
The y-axis of both panels represents the relative effect of growth in arsenate and azide (low metabolic condition) on the adsorption rate, η′, compared to the adsorption rate in the high metabolic condition, η, for hosts grown in glucose. Panel A displays this effect for each phage (x-axis), while panel B illustrates the relationship between the effect and the adsorption rate in glucose, η. The dashed line represents a fitted model on the metabolic state sensitive phages that examines and quantifies the correlation between η′/η and η.

Taken from Anastasios Marantos Kim Sneppen Stanley BrownNamiko Mitarai2025Viral commitment to infection depends on host metabolismeLife14:RP107825
https://doi.org/10.7554/eLife.107825.1

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 26/03/2026

📝 - This work aimed to compare the adsorption rate of 5 different E. coli phages (λ, ϕ80, M13, T6 and T5), under two different conditions: high and low metabolic activity. The authors described how, for 4 out of 5 phages, the ratio of adsorption is higher under high metabolic activity

Figure 1. Taken from Anastasios MarantosKim SneppenStanley BrownNamiko Mitarai2025Viral commitment to infection depends on host metabolismeLife14:RP107825
https://doi.org/10.7554/eLife.107825.1

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 19/03/2026

❓- Given that prophage induction appears dose- and context-dependent, i.e: variable cue exposure leads to some non-overlapping induction profiles, we’re left to wonder, how many molecular mechanisms are behind prophage dynamics in the gut? >100 years on, phages continue to surprise!!

Figure 4A The number of induced prophages per sample (condition in rows and isolates in columns). The isolate phylum is shown along the top bar. Actinomycetota is shown in yellow, Fusobacteriota in black, Bacillota in blue, Pseudomonadota in red and Bacteroidota in teal.

Cropped and highlighted (red squares) for emphasis on differences in phage induction results by different concentrations of the same stimuli

Modified from from Dahlman, S., Avellaneda-Franco, L., Rutten, E.L. et al. Isolation, engineering and ecology of temperate phages from the human gut. Nature 647, 698–705 (2025). https://doi.org/10.1038/s41586-025-09614-7

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 19/03/2026

✅- Interesting results stemmed from 1) The choice of gut-relevant induction conditions such as human cellular products (CaCo2) 2) Detection of DGRs targeting non-tail genes in the induced phages, likely related to phage counter-defense genes

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 19/03/2026

📝 - Using multiple stimuli, Dahlman and co. induced 134 temperate phages, representing 18% of the predicted prophages, in a collection of 252 human gut bacteria The authors characterize the induced phages and provide insight into factors governing the induction (or lack thereof) of prophages

Figure 1A Schematic of the methods used. (1) Induction of pure-culture bacterial isolates with standard induction agents. (2) Bacterial synthetic community co-cultured with and without Caco2 cell monolayer. (3) Induction of bacterial isolate pure cultures with cell culture medium, Caco2 cells or cell products

Cropped for emphasis on experimental design

Taken from Dahlman, S., Avellaneda-Franco, L., Rutten, E.L. et al. Isolation, engineering and ecology of temperate phages from the human gut. Nature 647, 698–705 (2025). https://doi.org/10.1038/s41586-025-09614-7

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 16/03/2026

The BBH lab has a new Doctor!!🚨🚨 Caroline Henrot, a member of our lab since 2022, defended her thesis last friday @pasteur.fr This work focused on the induction of prophages, and other bacterial interactions, in the gut microbiota. It was performed in collaboration with @inrae-france.bsky.social

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 09/03/2026

We have a new lab member at the BBH! Meet Brice Gastineau, a Master 2 student who'll be joining us for the next 7 months. He'll be working with our Lung team 🫁, particularly with the lung-on-chip model Welcome to the lab!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 26/02/2026

Today was Microbiology PhDay at @pasteur.fr where we highlighted the work of two of our PhD candidates Solène Ecomard and Ngoc-Minh-Chau Nguyen participated with their posters on Tail fiber and Lung-on-chip, showing some of the good work performed by them at the BBH 👏

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 21/01/2026

First post of 2026 for the BBH and it's a good one. This week, one of our PhD candidates, Caroline Henrot, took a big step in her journey by submitting her thesis document 🎉 Stay tuned for more news on her and her work, performed in collaboration with @inrae-france.bsky.social, throughout the year!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/12/2025

Last friday one of our lab's Postdocs, Nandita Sharma, presented her "Work in progress" talk at @pasteur.fr Her results deal with the role of bacterial competition in the microbiota and the factors at play determining its outcome. Really cool stuff that we'll be sharing in the near future

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 25/11/2025

We mentioned this a while back, so we thought we'd show you another photo from that day, taken from a different angle 😎 This documentary, featuring the boss, will premiere TONIGHT!!! at 21h in France 5 www.francetvpro.fr/contenu-de-p... www.francetelevisions.fr/et-vous/notr...

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/11/2025

We mentioned that we had 5 posters, right? Well, we saved this one for last because Ngoc-Minh-Chau Nguyen rocked the house and ended up leaving with a prize!!! 🏆 She was VERY lively while presenting her results so we were glad to see her work be recognized by the jury bsky.app/profile/phag...

Ngoc-Minh-Chau Nguyen receiving a prize for her poster titled "From mouse to man: building models to quantify the dynamic interactions between bacteriophages and bacteria on the pulmonary epithelium" at the "Phages in Nancy" conference

Ngoc-Minh-Chau Nguyen presenting her poster titled "From mouse to man: building models to quantify the dynamic interactions between bacteriophages and bacteria on the pulmonary epithelium" at the "Phages in Nancy" conference

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/11/2025

Eventually things got competitive, as we tried to assemble the lego phage It took us a while to put the whole thing together but look, we're not structural virologists, ok?

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/11/2025

Of course, not everything was work, the team also participated in the fun time at the gala dinner Although, as soon as the boss saw this little lego phage, we were back to the classroom 🤓

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/11/2025

We were also able to present two talks, one by Caroline Henrot and another by Nicolas Dufour (in the “Phages, MICI, Phagothérapie & Co” session) Fun fact: Caroline is actually from Nancy so she had to represent in her hometown and she did a very good job!!

Caroline Henrot, presenting her talk "Prophage-inducing bacteriocins are frequent in Escherichia coli intestinal isolates" at the "Phages in Nancy" conference

Nicolas Dufour, presenting his talk "Phagothérapie et infection pulmonaire : état des lieux et défis à relever" at the “Phages, MICI, Phagothérapie & Co” session, attached to the "Phages in Nancy" conference

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/11/2025

Our group was able to participate with 5 posters by members of our "gut" and "lung" teams There was lively discussion around them and we were happy to share our results You'll notice we only shared 4 posters in here ............ I wonder why🤔

Postdoc Nandita Sharma, presenting her poster "Interspecies competition between Citrobacter rodentium and Escherichia coli involves prophages" at the Phages in Nancy conference

Camille Sivelle, presenting her poster "Explorations on the mechanisms that bacteria deploy to survive a bacteriophage cocktail challenge" at the "Phages in Nancy" conference

Solene Ecomard presenting her poster "Structure-function relationships between bacteriophage tail fibers and host range: investigations with Nankovirus and Pakpunavirus infecting Pseudomonas aeruginosa" at the "Phages in Nancy" conference

Jose Alejandro Bohorquez presenting his poster "Interaction between bacteriophages and innate immunity as a determinant for the efficacy of phage therapy" at the "Phages in Nancy" conference

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 17/11/2025

We were glad to take part in the "Phages in Nancy" meeting last week, organized by site.phages.fr and hosted by Xavier Bellanger We'll share some of the highlights in this thread 🧵

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 11/11/2025

TL,DR We had a good time visiting some of our Portuguese colleagues this week, where we talked #phage and ran into some old and new faces Our visit had it all: good science, good food and even an unexpected seismic drill You can read all about it on this thread 🧵

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 11/11/2025

This picture was taken right before The Boss' presentation at @gimmfoundation.bsky.social We initially thought people were just a bit shy but, as it turned out, we were participating in the mandatory drills due to high seismic activity in the area 🤔 Safety first!!

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 11/11/2025

We also visited Isabel Gordo's lab at @gimmfoundation.bsky.social (Oeiras) and ran into a familiar face, @martalourenco21.bsky.social former PhD candidate at our lab

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 11/11/2025

We were happy to catch up with Luis @ltanoeiro.bsky.social, who's doing some of his PhD work over there under the supervision of Filipa and Jorge Vitor bsky.app/profile/phag...

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 11/11/2025

Last week, part of our team was busy at Bioisi (U. of Lisbon @cienciasulisboa.bsky.social ), talking all things #phage and #prophage with our collaborator Filipa Vale

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 11/11/2025

We also got to share some good times! Thanks a lot to Profs. Jorge Vitor and Filipa Vale, as well as our own Luis Tanoeiro @ltanoeiro.bsky.social for your hospitality

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 04/11/2025

🔵⚪🔴 - Dans le cadre du groupe de travail ApprentiPhages (réseau Phages.fr), nous avons le plaisir de partager quelques vidéos pédagogiques sur nos virus favoris : www.canal-u.tv/chaines/phag... D'autres thématiques phago-centrées à venir !

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Bacteriophage Lab of Institut Pasteur @phagepasteur.bsky.social · 04/11/2025

As part of the ApprentiPhages working group (Phages.fr network), we are pleased to share some educational videos about our favorite viruses: www.canal-u.tv/chaines/phag... More phage-related topics coming soon! Note: the videos are in French!”

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