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Oscar Wilkins

@oscargwilkins.bsky.social

Reposted by Oscar Wilkins

Reese Richardson @reeserichardson.bsky.social · 05/06/2026

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

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Reposted by Oscar Wilkins

Pietro Fratta @frattalab.bsky.social · 06/04/2025

Something different from the lab! We need better ALS biomarkers to minimise size, duration and cost of trials. Muscle MRI could work as it reliably detects progressive atrophy, and correlates with muscle strength loss. @uclqsion.bsky.social @uclbrainscience.bsky.social jnnp.bmj.com/content/earl...

jnnp.bmj.com

Muscle MRI quantifies disease progression in amyotrophic lateral sclerosis

Background and objectives Quantitative and operator-independent biomarkers of disease progression are urgently needed in amyotrophic lateral sclerosis (ALS) research. We assess the potential of skelet...

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Oscar Wilkins @oscargwilkins.bsky.social · 12/02/2025

PSA for anyone interested in growing E. coli for plasmid preps in 96 wells. (this is 1 ml of NEB Stable at 37C 240 RPM or 30C 180 RPM). Using TB enables you to obtain as much DNA as a standard miniprep, beautiful

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Oscar Wilkins @oscargwilkins.bsky.social · 30/01/2025

Can we make it a rule that when people publish papers involving generation of new plasmids, they put the plasmid sequence in the supplementary? Much more useful than "we digested plasmid A with enzyme B then ligated to C then digested with D then gel purified etc etc etc"

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Oscar Wilkins @oscargwilkins.bsky.social · 08/01/2025

We're so pleased that groups have been ordering our TDP-REG vectors from Addgene! Please be aware that splicing minigenes cannot easily be used with lentiviruses, because the lentiviral RNA can get spliced before viral packaging! Better to use piggybac or similar, AAV, or just transient transfection

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