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Nadja Wunsch

@nadwu.bsky.social

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PhD student @ COS Heidelberg Exploring Arabidopsis V-ATPase 🌱

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Reposted by Nadja Wunsch

John Rubinstein @johnrubinstein.bsky.social · 04/07/2026

New preprint! Karin Schumacher's postdoc Nadja used our SidK approach to purify Arabidopsis V-ATPase & Mariia Khamina determined its structure. The structure shows unique plant V-ATPase features and that TLDc proteins (here OXR5) bind V-ATPase in all eukaryotes. 🌱 tinyurl.com/mw9xaszk

Overall structure of Arabidopsis V-ATPase. (A) SDS-PAGE of purified V-ATPase. (B) Composite map of Arabidopsis V-ATPase in rotational state 1. (C) Atomic model of Arabidopsis V-ATPase in rotational state 1. (D) Cross sections of the V1 and VO regions in three rotational states. (E) Conformations of subunit H in V-ATPase structures of Arabidopsis V-ATPase (top) and the citrus fruit (bottom) V-ATPase.

V-ATPase from the TGN/EE interacts with the TLDc protein OXR5. (A) Composite map of Arabidopsis V-ATPase in rotational state 1 bound to OXR5 (green density). (B) AlphaFold3 model (left) and domain organisation (right) of OXR5. (C) VHA-a1-mNG is localized at the TGN/EE. OXR5-PmScarlet-I shows partial overlap with VHA-a1-mNG at the TGN/EE. Scale bar, 20 µm. Root tip elongation zone of 5-day-old Arabidopsis seedlings were analyzed by confocal laser scanning microscopy. (D-H) Atomic model of Arabidopsis V-ATPase interactions with OXR5.

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Reposted by Nadja Wunsch

John Rubinstein @johnrubinstein.bsky.social · 04/07/2026

New preprint! Karin Schumacher's postdoc Nadja used our SidK approach to purify Arabidopsis V-ATPase & Mariia Khamina determined its structure. The structure shows unique plant V-ATPase features and that TLDc proteins (here OXR5) bind V-ATPase in all eukaryotes. 🌱 tinyurl.com/mw9xaszk

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