Mathias Munschauer @munschauerlab.bsky.social · 12/02/2024Using SHIFTR, we identify interactions of the 5′ and 3′-terminal regions of authentic SARS-CoV-2 RNAs produced during infection and accurately recover known and novel RNA interactors. 120
Mathias Munschauer @munschauerlab.bsky.social · 12/02/2024Proteins bound to a specific RNA can be released from interphase extracts by selectively degrading the RNA of interest, for instance with RNase H + specific DNA probes. Loss of the RNA leads to a SHIFT of crosslinked proteins to the organic phase in the next extraction step. 110