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Milstein Lab @ UofT

@milsteinlab.bsky.social
1.1K followers 535 following 40 posts

Biological Physics at #UofT. We study (primarily) bacteria using single-molecule and single-cell techniques. www.utm.utoronto.ca/milsteinlab

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Milstein Lab @ UofT @milsteinlab.bsky.social · 27/07/2026
We're hiring at the Assistant #Professor level in #Medicinal / #Biological #Chemistry and Chemical Biology. Please share with anyone you think might be interested. jobs.utoronto.ca/job/Mississa...
jobs.utoronto.ca
Assistant Professor - Medicinal/Biological Chemistry or Chemical Biology (Impact+ Emerging Leader)
Assistant Professor - Medicinal/Biological Chemistry or Chemical Biology (Impact+ Emerging Leader)
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Milstein Lab @ UofT @milsteinlab.bsky.social · 26/05/2026
Just out....ahead of print...our review article in #MBoC on how mechanical effects shape #bacterial dynamics and #ecology in dense and confined environments www.molbiolcell.org/doi/10.1091/...
molbiolcell.org
Bacterial ecology in confined and spatially structured in vitro habitats | Molecular Biology of the Cell
Bacteria commonly coexist within communities where they compete for limited space and resources. These communities play an essential role in supporting a diversity of critical ecosystems from regulati...
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Milstein Lab @ UofT @milsteinlab.bsky.social · 14/03/2026
Another successful collaboration with the Beharry Lab on light activated #antimicrobials chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1...
chemistry-europe.onlinelibrary.wiley.com
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Reposted by Milstein Lab @ UofT
Biophysical Society of Canada @biophyscanada.bsky.social · 01/03/2026
The Biophysical Society of Canada is pleased to announce that Dr. Sarah Rauscher @sarahrauscher.bsky.social is the recipient of the 2026 Early Career Investigator Award. Congratulations!!
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Milstein Lab @ UofT @milsteinlab.bsky.social · 27/02/2026
#INSPIRE2026 is now open for registration. Spend 2-weeks getting hands on experience in #QuantitativeBiology and #ResearchTranslation @utm.utoronto.ca www.utm.utoronto.ca/dean/inspire
utm.utoronto.ca
INSPIRE | Office of the Vice-Principal Academic and Dean
U of T's Interdisciplinary Science Program in Research & Entrepreneurship Discover the Quantitative BiosciencesJune 15–26, 2026Join a two‑week immersive research experience where you’ll explore unanswered biological questions inside state‑of‑the‑art laboratories.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 27/02/2026
Just out…our recent preprint on how she shapes of #bacteria can affect the stability of the #microbial communities they form www.biorxiv.org/content/10.6...
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Milstein Lab @ UofT @milsteinlab.bsky.social · 30/07/2025
Learn more about #INSPIRE, a program we developed at the interface of #biophysics, drug development, #innovation and #entrepreneurship www.youtube.com/watch?v=EmwL...
youtube.com
2025 INSPIRE Scholars Program
YouTube video by Office of VP Research & Innovation at UTM
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Milstein Lab @ UofT @milsteinlab.bsky.social · 29/06/2025
At long last, are newest article on "Enhancing the applied force and range of axial optical tweezers” is out. #singlemolecule #biophysics www.cell.com/biophysrepor...
cell.com
Enhancing the applied force and range of axial optical tweezers
Axial optical tweezers provide a natural geometry for performing biomechanical assays, such as rupture force measurements of protein binding. Axial traps, however, are typically weaker than their late...
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Milstein Lab @ UofT @milsteinlab.bsky.social · 17/06/2025
Our experimental lab w/ the Hilfinger lab @uoft.bsky.social are jointly looking to hire a #postdoc in experimental biological #physics. Exploring noise and dynamics within single-cells and bacterial populations. Please share with anyone you think might be interested.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 02/06/2025
Here’s a great opportunity if you know of anyone looking for a postdoc.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 15/04/2025
This is based on our publication in #BioinformaticsAdvances, but is much much faster, does all the preprocessing for you, and has a nice GUI. academic.oup.com/bioinformati...
academic.oup.com
An expectation–maximization approach to quantifying protein stoichiometry with single-molecule imaging
AbstractMotivation. Single-molecule localization microscopy (SMLM) is a super-resolution technique capable of rendering nanometer scale images of cellular
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Milstein Lab @ UofT @milsteinlab.bsky.social · 15/04/2025
If anyone is interested, we’ve developed a nice GUI in Python for molecular counting with #SMLM. Just give it a localization file and it will estimate the monomer/dimer fraction. github.com/thericshi/pr...
github.com
GitHub - thericshi/protein-stoichiometry-quantifier
Contribute to thericshi/protein-stoichiometry-quantifier development by creating an account on GitHub.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 07/03/2025
A big shout out to everyone who’s marching at today’s #standupforscience events.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 06/03/2025
Another successful collaboration with the Beharry lab on #antimicrobial photodynamic therapy. This just out in @rscmedchem.rsc.org: pubs.rsc.org/en/content/a...
pubs.rsc.org
Nitroreductase-activatable photosensitizers for selective antimicrobial photodynamic therapy
Antimicrobial photodynamic therapy (aPDT) utilizes light, oxygen and a photosensitizer (PS) to enact cell death via the production of reactive oxygen species (ROS). This mechanism of cell death, via o...
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Reposted by Milstein Lab @ UofT
Dmytro Mishkin @ducha-aiki.bsky.social · 25/02/2025
Sometimes reading Hamming makes me sad, because I recognize myself in this quote.
Question: How much effort should go into library work?

Hamming: It depends upon the field. I will say this about it. There was a fellow at Bell Labs, a very, very, smart guy. He was always in the library; he read everything. If you wanted references, you went to him and he gave you all kinds of references. But in the middle of forming these theories, I formed a proposition: there would be no effect named after him in the long run. He is now retired from Bell Labs and is an Adjunct Professor. He was very valuable; I'm not questioning that. He wrote some very good Physical Review articles; but there's no effect named after him because he read too much. If you read all the time what other people have done you will think the way they thought. If you want to think new thoughts that are different, then do what a lot of creative people do - get the problem reasonably clear and then refuse to look at any answers until you've thought the problem through carefully how you would do it, how you could slightly change the problem to be the correct one. So yes, you need to keep up. You need to keep up more to find out what the problems are than to read to find the solutions. The reading is necessary to know what is going on and what is possible. But reading to get the solutions does not seem to be the way to do great research. So I'll give you two answers. You read; but it is not the amount, it is the way you read that counts.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 19/02/2025
Here’s an event I would highly recommend. Just around the corner if you’re in the GTA.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 06/02/2025
actionnetwork.org/petitions/de...
actionnetwork.org
Dear Future Premier, science needs to be a priority.
I just signed a letter to put science and evidence-informed decision-making at the heart of our provincial elections!🗳️🔬 In the upcoming elections in Ontario, it's crucial that our future premiers pr...
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Milstein Lab @ UofT @milsteinlab.bsky.social · 06/02/2025
Lots of #imaging and #microscopy workshops coming up in Canada. Just thought I would share. Thanks @bioimagingna.bsky.social and #CanadaBioimaging
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Milstein Lab @ UofT @milsteinlab.bsky.social · 21/01/2025
Here’s a bit more information on #INSPIRE2024. Feel free to print out and post.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 10/01/2025
Registration for #INSPIRE2025 is open to all #Physics, #Chemistry, and #Biology undergraduates with an interest in Quantitative Biological and Translational Sciences. Work directly in labs @uoft.bsky.social @uoftmississauga.bsky.social while also learning about #innovation and #entrepreneurship.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 07/01/2025
Look what I just found in my mailbox. It’s nice to finally see this is print.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 10/12/2024
Pleased to see that #SpinUP made the news. Lot’s of innovative research translation efforts happening here @uoftmississauga.bsky.social @uoft.bsky.social www.theglobeandmail.com/business/art...
theglobeandmail.com
Why Canada excels at medical tech startups
The Globe and Mail offers the most authoritative news in Canada, featuring national and international news
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Milstein Lab @ UofT @milsteinlab.bsky.social · 09/12/2024
Forget a clean room, we can do most of our larger feature #microfluidics on a resin based #3Dprinter. Here’s a PDMS mold for a serpentine mixer that you can even bake at 120C.
Serpentine mixer mold
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Milstein Lab @ UofT @milsteinlab.bsky.social · 04/12/2024
Finally starting to get this system working. While these movies might not look too impressive, they make me feel good. #microfluidics #biophysics
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Milstein Lab @ UofT @milsteinlab.bsky.social · 21/11/2024
We’re looking to hire a theoretical #biological #physicist at either the Assistant or Associate professor level. The post closes soon, so please spread the word. #Toronto is a great place to live and work. jobs.utoronto.ca/job/Toronto-... jobs.utoronto.ca/job/Toronto-...
jobs.utoronto.ca
Assistant Professor - Theoretical Biological Physics
Assistant Professor - Theoretical Biological Physics
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Milstein Lab @ UofT @milsteinlab.bsky.social · 18/11/2024
Congrats to Zhang and Tianyi on successfully pulling off their #PhDs! Now I need more students…so if you know of anyone...
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Milstein Lab @ UofT @milsteinlab.bsky.social · 14/11/2024
And here’s a movie of one of our microchannel competition experiments. It shows how spontaneous ordering of the cells can lead to a quasi-stable, mixed bacterial community.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
Oh, and finally, our side hustle is working with medicinal chemists to develop novel #antimicrobials. Here’s our latest effort with Andrew Beharry's lab chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
chemistry-europe.onlinelibrary.wiley.com
Antimicrobial Efficacy of Photocaged β‐Lapachone in Bacillus subtilis Biofilms
Photocaged β-lapachone uncages effectively in B. subtilis biofilms using λ=420 nm light, causing catalase inhibition, leading to inhibition of growth. With antimicrobial resistance on the rise, an al....
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
And the other half of our time is spent developing single-molecule imaging, with a particular emphasis on counting molecules and defining the oligomerization state of membrane proteins (particularly #GPCRs).
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
We have also been developing ecological theories of bacterial competition within confined environments
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
Our most recent work looked at the mechanical effects on competition between two model bacteria competing for space and resources within microchannels.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
Since we’re starting over here, how about a quick introduction to our research lab. We’re interested in a range of quantitative imaging, from imaging single-molecules to competitive populations of bacteria.
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
Updated our preprint on #singlemolecule force spectroscopy using axial tweezers. www.biorxiv.org/content/10.1...
biorxiv.org
Enhancing the Applied Force and Range of Axial Optical Tweezers
Axial optical tweezers provide a natural geometry for performing biomechanical assays such as rupture force measurements of protein binding. Axial traps, however, are typically weaker than their later...
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Milstein Lab @ UofT @milsteinlab.bsky.social · 13/11/2024
Our lab is now on Bluesky Social. Trying to find a place to share our science (that isn't evil).
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