Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 25/06/2026We've updated our cleared-tissue direct-view oblique plane microscopy preprint to include our latest resolution-enhancing results. Now the Nyquist sampling of our camera is no longer a limitation and so we obtain a diffraction-limited 2 μm lateral resolution! www.biorxiv.org/content/10.6... 1153
Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 02/06/2026We present: Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution www.biorxiv.org/content/10.6... 14217
Reposted by Miguel MestreMeg Clapperton @meganclapperton.bsky.social · 24/04/2026It was an absolute honour to win the #BSCB poster prize at #DynamicCellVI ! Its my first ever dynamic cell and I already can't wait for the next one! Make sure to come to our other talk today on using this tech to study endosomal dynamics? 0358
Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 13/03/2026Myself and @immunoah.bsky.social are looking to hire a postdoctoral scientist to build a next-generation high-throughput high-resolution light sheet microscope for imaging entire cleared organs/tissues and to further develop live & fixed tissue clearing methods. www.nature.com/naturecareer... 01916
Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 01/12/2025For an internal talk, I wanted an easy way to disprove the prevailing myth that expansion microscopy gels expand due to repulsion between charged polymer strands. I did a quick calculation of the supposed energy released during a standard experiment and it's equivalent to a small nuclear bomb... 5142
Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 16/09/2025The final version of our multispectral microscopy manuscript is now online at Nature Photonics: www.nature.com/articles/s41... The main additions are some further comparisons to yet more unmixing algorithms, in which we find that our algorithm is still the most accurate.nature.comMultispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature PhotonicsA tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with... 13616
Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 15/09/2025We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens: 37632
Reposted by Miguel MestreLMB-EPFL Graduate Life Sciences Symposium 2026 @lmbphdsymposium.bsky.social · 22/05/2025Our symposium is the perfect setting to showcase your work. Submit an abstract and apply for a talk or a poster now at the registration link in our bio. Make sure to submit your abstract by the 10th of June. 021
Reposted by Miguel MestreLMB-EPFL Graduate Life Sciences Symposium 2026 @lmbphdsymposium.bsky.social · 21/05/2025Save the Date 📍 Where: MRC-LMB, Cambridge or Online (Zoom) 🗓️ When: 10th and 11th of July 🧪 Themes: Cell Biology, Structural Biology, Immunology, Nucleic Acids and Neuroscience. 🤝 In collaboration with students at Institut Pasteur 584
Miguel Mestre @miguelcmestre.bsky.social · 13/02/2025We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...biorxiv.orgDirect-view oblique plane microscopyThe ability to rapidly image mesoscopic samples is critically important for many areas of biological research. Owing to its high throughput and gentle, volumetric imaging nature, light-sheet fluoresce... 074
Reposted by Miguel MestreJames Manton @jamesdmanton.bsky.social · 12/02/2025We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...biorxiv.orgDirect-view oblique plane microscopyThe ability to rapidly image mesoscopic samples is critically important for many areas of biological research. Owing to its high throughput and gentle, volumetric imaging nature, light-sheet fluoresce... 45715