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Margaret Schroeder

@meschro.bsky.social
78 followers 118 following 12 posts

neuroscience postdoc in the Darnell Lab at Rockefeller 👩‍🔬 | astrocytes 🧠⭐ | surviving synovial sarcoma🎗

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Reposted by Margaret Schroeder
Leanne Holt @holtlm90.bsky.social · 29/09/2026
My lab is recruiting a postdoctoral researcher! Interested in astrocyte transcriptional regulation and addiction? We study how astrocytic transcriptional, epigenetic, and chromatin regulation contributes to substance abuse disorders. DM or email me! www.l-holt-lab.com
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Margaret Schroeder @meschro.bsky.social · 21/11/2025
Wow, so fun to think back to that! Thank you so much, Ed, for all your help along the way!
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Reposted by Margaret Schroeder
Yang Tan Collective @yangtancollective.bsky.social · 20/11/2025
A new cross-species atlas created by Yang Tan researchers reveals how astrocytes—star-shaped cells and the brain’s most abundant non-neuronal cells—change across space and time. This resource gives scientists an essential lens for probing brain development, function, and disease.
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
Code on GitHub (github.com/Feng-Lab-MIT...) and images on BossDB (bossdb.org/project/schr...).
github.com
GitHub - Feng-Lab-MIT/AstrocyteHeterogeneity: scripts for analysing snRNAseq, FISH, and ExR data accompanying Schroeder et al., 2025
scripts for analysing snRNAseq, FISH, and ExR data accompanying Schroeder et al., 2025 - Feng-Lab-MIT/AstrocyteHeterogeneity
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
Raw data (fastqs, h5 count matrices) on NeMO (data.nemoarchive.org/biccn/grant/...) and GEO (www.ncbi.nlm.nih.gov/geo/query/ac...).
data.nemoarchive.org
Index of /biccn/grant/u01_feng/feng/transcriptome/sncell/10x_v3.1
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
Processed data on the Broad Institute Single Cell Portal, searchable and interactive: singlecell.broadinstitute.org/single_cell/... (mouse) and singlecell.broadinstitute.org/single_cell/... (marmoset).
singlecell.broadinstitute.org
A multi-region transcriptomic atlas of developmental cell type diversity in mouse brain - Single Cell Portal
We used 10x Genomics Chromium single-nucleus RNA sequencing to characterize the molecular diversity of brain cells across six developmental stages (from late fetal to aged adult) and four brain regions (prefrontal cortex, motor cortex, striatum, and thalamus) in the mouse brain. Each developmental time point and region combination (except for aged) has 3 biological replicate donors. Nuclei are NOT enriched (e.g., FACS sorted) for any particular population and contain neurons, glia, and vasculature populations.
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
Though we focused our analysis on astrocytes, we did not sort or select for any particular cell type in our prep. Our dataset is a rich, publicly available resource containing all neural cell types - please explore via the links below, and feel free to reach out with any questions!
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
We then virally labeled astrocytes with GFP and used expansion microscopy to visualize their detailed morphology (it was so fun to take these images!). We quantified their morphological differences across regions in mouse.
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
Looking across species, we found broad conservation of cell types. However, several marmoset cell types appeared more mature than their mouse counterparts at birth, and the gene expression signatures underlying astrocyte regional heterogeneity diverged significantly between mouse and marmoset.
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
As others had shown, we found that astrocytic regional heterogeneity is patterned embryonically. What we were surprised to find was just how much regional astrocyte gene expression signatures change over postnatal development, likely in support of local neuronal circuits.
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
To answer this question, we profiled millions of single nuclei with 10x Genomics snRNAseq across brain regions (motor and prefrontal cortices, striatum, and thalamus) and developmental time (from late embryonic to aged time points) in mouse and marmoset.
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
What started out as an exploration of astrocyte regional heterogeneity in the young adult marmoset led us to ask, how early in development is astrocyte regional heterogeneity patterned, and how does it change over developmental time and across mammalian species?
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Margaret Schroeder @meschro.bsky.social · 20/11/2025
I’m so excited to share my first-author PhD paper, out today in Neuron as a NeuroResource! A huge thanks to all of my co-authors, and of course to my wonderful mentors Guoping Feng, @fennak.bsky.social and @eboyden3.bsky.social. www.cell.com/neuron/fullt...
cell.com
A transcriptomic atlas of astrocyte heterogeneity across space and time in mouse and marmoset
In this NeuroResource, Schroeder et al. present a transcriptomic atlas across brain regions and developmental time points in mouse and marmoset. Detailed analysis focused on astrocytes revealed that t...
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Margaret Schroeder @meschro.bsky.social · 16/11/2024
Very excited to share multiExR!
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