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Margo Le @margole.bsky.social · 04/09/2026

Conclusions: OFC→M2 is necessary to translate cued reward memories into goal-appropriate instrumental rules. Thank you to everyone that contributed to this work! @ronkeiflin.bsky.social

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Margo Le @margole.bsky.social · 04/09/2026

At test, reward-predictive cues biased rule selection, promoting the rule associated with the predicted outcome. Silencing the orbitofrontal-to-secondary motor pathway disrupted this cue-guided *rule* selection. Critically the same manipulation spared cue-evoked *action* selection in PIT paradigm.

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Margo Le @margole.bsky.social · 04/09/2026

Rats learned two different rules for completing a 3-step action sequence (delayed nonmatch to sample or visual rule), with each rule leading to a distinct reward outcome. www.keiflinlab.com/instrumental...

keiflinlab.com

Instrumental rules task — KEIFLIN LAB

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Margo Le @margole.bsky.social · 04/09/2026

To bring this higher-order level of behavioral organization into the lab, we adapted the iconic Pavlovian-to-Instrumental Transfer (PIT) paradigm and developed the Pavlovian-to-Rules-to-Instrumental Transfer (sPRInT).

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Margo Le @margole.bsky.social · 04/09/2026

But real-world reward pursuit is rarely a choice between isolated actions. Different goals often require different strategies -or higher-order rules- that organize multiple actions into a coordinated sequence.

static.klipy.com

Tex the Dog: Arko Obstacle Course Dog Show

ALT: Tex the Dog: Arko Obstacle Course Dog Show

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Margo Le @margole.bsky.social · 04/09/2026

Classic decision-making tasks (in rodents) often reduce choice to two competing actions: press the left lever or press the right lever.

static.klipy.com

Nervous Two Buttons GIF

ALT: Nervous Two Buttons GIF

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Margo Le @margole.bsky.social · 04/09/2026

Conclusions: OFC→M2 necessary to translate cued reward memories into goal-appropriate instrumental rules. Thank you to everyone who contributed to this work! @ronkeiflin.bsky.social

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Margo Le @margole.bsky.social · 04/09/2026

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Margo Le @margole.bsky.social · 04/09/2026

keiflinlab.com

Instrumental rules task — KEIFLIN LAB

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Margo Le @margole.bsky.social · 04/09/2026

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Margo Le @margole.bsky.social · 04/09/2026

static.klipy.com

Tex the Dog: Arko Obstacle Course Dog Show

ALT: Tex the Dog: Arko Obstacle Course Dog Show

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Margo Le @margole.bsky.social · 04/09/2026

Classic decision-making tasks (in rodents) often reduce choice to two competing actions: press the left lever or press the right lever.

static.klipy.com

Nervous Two Buttons GIF

ALT: Nervous Two Buttons GIF

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Reposted by Margo Le

Ron Keiflin @ronkeiflin.bsky.social · 23/04/2026

Had fun working with @margole.bsky.social on this eLife Insight piece. Also check out the original paper by Zong et al. (Schoenbaum lab): elifesciences.org/articles/109...

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