The team developed a high-throughput platform to rapidly measure how hundreds of viral mutation combinations affect antibody binding, generating 31,740 quantitative affinity data points. The resulting...

T7 RNA polymerase is widely used in synthetic biology, but tools to precisely regulate it are limited. Here, authors engineer a modular T7 RNAP regulatory toolbox for programmable repression, activati...

Building a self-replicating biochemical system remains a challenge in synthetic biology. Here the authors demonstrate that the cell-free protein synthesis PURE system can be reconstituted from protein...

Building a universal biochemical constructor, an autonomously self-replicating biochemical system, is a major challenge in synthetic biology. The PURE cell-free system is an ideal starting point for e...

Cell-free systems have emerged as a powerful platform for protein production, characterization, and bottom-up construction of artificial cells, offeri…

SARS-CoV-2 variants continue to threaten public health, necessitating the study of cumulative and epistatic effects of receptor-binding domain (RBD) mutations on antibody evasion. We present a high-th...