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Reposted by Ludovico Rizzuti

ME Torres-Padilla @metorrespadilla.bsky.social · 15/07/2025

🎤 Apply to speak @our TALENT FORUM by July 31! Showcase YOUR research in Epigenetics & NucleicAcids @www.helmholtz-munich.de & connect with leading scientists 🧳Travel+accommodation covered if selected! 📝 Info+how to apply: lnkd.in/drkRvz7N #postdoc #career #groupleader #HelmholtzMunich #research

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Reposted by Ludovico Rizzuti

Boyan Bonev @boyanbonev.bsky.social · 04/04/2025

Can a stem cell change its epigenome while proliferating? In collaboration with the lab of Amos Tanay, we demonstrate that yes, the epigenome of neural stem cells (NSCs) is continuously remodeled, across multiple layers, during mouse corticogenesis. 1/14 www.biorxiv.org/content/10.1...

biorxiv.org

Neural stem cell epigenomes and fate bias are temporally coordinated during corticogenesis

The cerebral cortex orchestrates complex cognitive functions, yet how its distinct temporal lineages are molecularly patterned during development remains unresolved. Here, we integrate single-cell tra...

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Reposted by Ludovico Rizzuti

Alessandro Vitriolo @alessandrovitriolo.bsky.social · 03/04/2025

So glad to see this work preprinted. Thanks @ludoritz.bsky.social for spearheading this massive effort with me and Mariana. There are so many good things in this paper. Thanks to Giuseppe and Raymond for guiding us. Thanks to collaborators, and #HVDAS families. #ADNP #neurodevelopment #multiomics

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

Special thanks to the Testa Lab people who shared this journey with me: Marlene Pereira, @sebastianotrattaro.bsky.social, @michelegabriele.bsky.social, Francesco Dossena, Erika Tenderini and the whole amazing group of people I've been so fortunate to work with.

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

Thanks to Giuseppe and Raymond for bringing together such an enthusiastic and international team to work on this project, and for the smooth collaboration.

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

GRNs computed from multiome profiling in HVDAS cortical organoids prioritize key AKG-bound TFs targets whose in silico KO rescues the accelerated maturation phenotype.

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

Patient-derived cortical organoids harboring HVDAS-causing ADNP mutations show morphological and functional impairments such as smaller-sized organoids, and reduced mitotic capacity of neuronal progenitors coupled with accelerated neuronal differentiation.

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

ADNP DNA-binding motif in iPSCs and hNSCs differs from the one of CTCF, as previously reported in mESCs. MS and co-IP show that in humans ADNP interacts with GTF2I and KDM1A forming the "AKG" complex. Plus, ADNP KO in hNSCs show derepression of KDM1A-bound promoters.

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

Patient-derived iPSCs show decreased genome-wide ADNP occupancy, prevalently leading to gene activation at ADNP-lost sites. Moreover, ADNP represses its targets through regulatory regions rich in primate-specific transposable elements (TEs).

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

We modelled ADNP across iPSCs, neural stem cells, neural crest stem cells, and cortical organoids, integrating patient-derived models with endogenous gene editing.

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Ludovico Rizzuti @ludoritz.bsky.social · 03/04/2025

I'm happy to share our work on the neurodevelopmental impact of ADNP mutations in ASD and beyond. It has been an exciting ride to share with @alessandrovitriolo.bsky.social, Mariana, and the people of the Testa and Poot Labs. Full paper here: www.biorxiv.org/content/10.1... Thread below 🧵

biorxiv.org

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