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Ludwig Sinn

@lrsinn.bsky.social

114 followers 165 following 8 posts

PostDoc @RalserLab #systemsbiology, #lcms, structural and quantitative high-throughput #proteomics+PTMs. Past @DemichevLab & @RappsilberLab

Posts Replies Media

Ludwig Sinn @lrsinn.bsky.social · 17/11/2025

It was super nice to be part of such challenging undertaking in integrative structural biology. For anyone interested in a mechanistic understanding of mRNA decay via CCR4-Not and IDRs in general, this is for you!

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Reposted by Ludwig Sinn

Lori Passmore @lapassmore.bsky.social · 15/11/2025

This work is now officially published @natsmb.nature.com with some fun new analyses included in the revised version. Congratulations to all the authors! @connyyu.bsky.social @rappsilber.bsky.social @lrsinn.bsky.social @fjoreilly.bsky.social www.nature.com/articles/s41...

nature.com

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Ludwig Sinn @lrsinn.bsky.social · 03/06/2025

The "641 Hz" in the 750 Da/s-5 Da Q1-ZT Scan DIA method corresponds to the effective TOF-MS/MS scan rate after correcting for overheads from data binning, TOF-MS, and some other processes SCIEX knows more about

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Ludwig Sinn @lrsinn.bsky.social · 03/06/2025

Hi Hamish, to the best of my knowledge, "dwell time" here is defined as the time it takes to transfer ions of a certain m/z in the Q1. We tried to match ZT Scan's dwell time to ZenoSWATH's MS2 accumulation times for a fair comparison of both methods. Indeed, 0.67 ms is the duration of a Zeno pulse.

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Ludwig Sinn @lrsinn.bsky.social · 14/05/2025

Beyond support by SCIEX, we acknowledge funding from the BMBF, MSCoreSys, the SFB TRR186 and @erc.europa.eu. A special Thanks to everyone involved from the Ralser Lab (ralser.group), SCIEX @sciex.bsky.social, as well as @vadim-demichev.bsky.social and @christophmessner.bsky.social.

ralser.group

THE RALSER LAB

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Ludwig Sinn @lrsinn.bsky.social · 14/05/2025

We are super thrilled to see ZT Scan DIA leading to insight from miniscule proteome samples, in clinical proteomics and functional proteomics studies that depend on highly precise protein quantification from large sample series.

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Ludwig Sinn @lrsinn.bsky.social · 14/05/2025

The meticulous development with our collaborators from SCIEX (@stevetate_absx and the magnificent team) was worth the effort, seeing gains between 30-40% in Protein IDs and improved quantitative performance in high-throughput, as well as with low-input proteomics applications.

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Ludwig Sinn @lrsinn.bsky.social · 14/05/2025

Combining the benefits of ZenoTrap and Q1 scanning

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Ludwig Sinn @lrsinn.bsky.social · 14/05/2025

I am excited to see our performance assessment of the successor of Scanning SWATH on the Zeno TOF7600+ mass spectrometer - ZT Scan DIA - pre-printed (www.biorxiv.org/content/10.1...

biorxiv.org

Performance Characteristics of Zeno Trap Scanning DIA for Sensitive and Quantitative Proteomics at High Throughput

Proteomic experiments, particularly those addressing dynamic proteome properties, time series, or genetic diversity, require the analysis of large sample numbers. Despite significant advancements in p...

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