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Giordano Lippi @lippilab.bsky.social · 07/04/2026

The Lippi lab is hiring a postdoctoral associate! We built new technologies to study post-transcriptional regulation in the healthy and diseased brain. We are recruiting motivated and talented scientists who will use these tools to discover new biology and new therapies.

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Giordano Lippi @lippilab.bsky.social · 24/02/2026

This was a gargantuan but incredibly satisfying collaboration, which was critically supported by a BRAIN Initiative grant, among other funding mechanisms.

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Giordano Lippi @lippilab.bsky.social · 24/02/2026

Finally, we found that CA3 neurons translate at higher levels compared to CA1, despite CA1 expressing more transcripts. Many highly translated genes are ribosomal machinery genes, resulting in a different translational landscape that could matter for hippocampal function.

Transcriptional and translational dynamics in CA3 vs CA1 neurons. Highlighted in red are ribosomal proteins, and elongation factors

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Giordano Lippi @lippilab.bsky.social · 24/02/2026

Excitingly, we found that, for the same gene, the isoform displaying the highest translation has a longer 3'UTR than the lowest-translated isoform. This seems to result from complex interactions among splicing and negative and positive translational regulators (e.g., microRNAs and nELAVL).

Cadm2 highly-translated isoform has a longer 3'UTR that hosts nELAVL binding sites

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Giordano Lippi @lippilab.bsky.social · 24/02/2026

We performed simultaneous single-cell transcriptional and translational profiling of the mouse hippocampus. We found that post-transcriptional mechanisms 'clean up' transcriptional noise. We identified a subset of neurons at high translational state, possibly a proxy for plasticity.

Plasticity genes Grin2b and Celf4 are highly translated in a subset of neurons, but the RNA level is uniform.

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Giordano Lippi @lippilab.bsky.social · 24/02/2026

Paired with long-read RNAseq, Ribo-STAMP can measure the translation potential of transcript isoforms. Since defects in alternative splicing are strongly linked to neuropsychiatric disorders, understanding how protein production is affected could suggest novel disease mechanisms.

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Giordano Lippi @lippilab.bsky.social · 24/02/2026

Brain cells display the highest levels of post-transcriptional gene expression regulation. Yet, to understand the underlying mechanisms, we need tools that match their incredible cell diversity. The Yeo lab developed Ribo-STAMP to measure translation at single-cell resolution.

The RNA editor APOBEC1 is fused to a ribosomal protein. During translation, Ribo-STAMP leaves a mark in the form of a C-to-U edit.

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Reposted by Giordano Lippi

Scripps Research @scripps.edu · 08/09/2025

In our first pitch session, Ryan Shenvi, PhD, @lippilab.bsky.social, PhD, and Li Ye, PhD, will each share their approach to understanding the brain—from neuroactive compound synthesis, to microRNAs in circuit development, to brain energetics—and will take questions after each of their pitches.

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Giordano Lippi @lippilab.bsky.social · 21/05/2025

First author @norjin.bsky.social has a different version of the birth of this project. A classic case of convergent evolution.

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Giordano Lippi @lippilab.bsky.social · 21/05/2025

How fitting that the Preview of our Neuron paper was written by the one and only Roy Sillitoe. I saw him giving a talk at the 2018 Neural Development GRC, and his incredible data, storytelling, and enthusiasm convinced me to use Purkinje Cells as a model system. www.cell.com/neuron/fullt...

cell.com

Rooted in regulation: MicroRNAs as gardeners of the Purkinje cell dendritic arbor

Zolboot et al.1 developed tools to manipulate and map microRNA function in cerebellar Purkinje cells with temporal precision, revealing key roles in dendritic development and circuit connectivity. The...

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Giordano Lippi @lippilab.bsky.social · 18/05/2025

Wow! Great stuff Debby congrats!

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Reposted by Giordano Lippi

Joris de Wit @synapse-lab.bsky.social · 08/04/2025

Postdoc opportunity in the heart of Europe! Check out the flyer below. Join our team on an international project with Anthony Holtmaat exploring synaptic plasticity and specificity in cortical and thalamocortical circuits.

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Reposted by Giordano Lippi

Scripps Research @scripps.edu · 03/04/2025

The Lipp Lab (@lippilab.bsky.social) has uncovered how microRNAs guide the development of Purkinje cells—rare neurons tied to movement and autism. Published in Neuron, a @cellpress.bsky.social journal, the study maps how precise timing and gene regulation shape these cells’ identity and structure.

scripps.edu

How microRNAs act as a “blueprint” for the developing brain

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Giordano Lippi @lippilab.bsky.social · 02/04/2025

Very grateful to Bennie Babayan at @cp-neuron.bsky.social for a fantastic job as editor, and the funding that supported this project. NIH, @autismspeaks.org and @cziscience.bsky.social.

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Giordano Lippi @lippilab.bsky.social · 02/04/2025

My favorite part is finding cell type-specific post-transcriptional mechanisms that drive morphological diversification among neurons. For example, strong repression in Purkinje cells of genes expressed also in pyramidal neurons is necessary for exuberant arborization! 🤯

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Giordano Lippi @lippilab.bsky.social · 02/04/2025

Delighted to see our paper finally out in @cp-neuron.bsky.social! Together with Ian MacRae, we developed a new toolbox to study microRNAs and used it to find new mechanisms of Purkinje cell development. Please see the tweetorial from researcher extraordinaire @norjin.bsky.social for details.

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Giordano Lippi @lippilab.bsky.social · 04/03/2025

Yes! See you there. I will send you an email for introduction

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Giordano Lippi @lippilab.bsky.social · 04/03/2025

My postdoc Fede could help. Let me know if you still need it

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Giordano Lippi @lippilab.bsky.social · 25/02/2025

Congratulations Dr. Du! The second student of the Lippi lab to successfully defend her PhD. Very proud PI. And of course, we celebrate in style.

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Giordano Lippi @lippilab.bsky.social · 01/12/2024

As it ever been OFF is the real question? Thanks for doing such a great job at the CZI webinar

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