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Linda Decker @lindithium.bsky.social · 01/09/2026

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Linda Decker @lindithium.bsky.social · 01/09/2026

Together, both studies demonstrated that #spatialomics can now be extended by mechanical mesasurements. We highlighted that tissue stiffness matters across species, ages and pathologies and should therefore be considered more regularly in clinical and fundamental research.

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Linda Decker @lindithium.bsky.social · 01/09/2026

We are especially excited to have uploaded this back-to-back with @huanting-ong.bsky.social and @jennyoung.bsky.social at the @mbisg.bsky.social who performed nano-indentation paired with Visium HD in the ageing mouse ovary! doi.org/10.64898/202...

doi.org

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Linda Decker @lindithium.bsky.social · 01/09/2026

Our workflow enabled us to identify candidate genes associated with increased tissue stiffness in colorectal cancer liver metastasis: 𝘛𝘎𝘍𝘉𝘐, 𝘛𝘍𝘍3, 𝘛𝘍𝘍1, and 𝘗𝘙𝘈𝘗1. These genes could potentially become valuable targets for stiffness-reducing mechanomedicines.

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Linda Decker @lindithium.bsky.social · 01/09/2026

We probed two 20 µm thick directly adjacent cryosections each from 10 colorectal cancer liver metastasis patients. Using AFM and Visium Spatial Transcriptomics we created high resolution stiffness and gene expression maps that we spatially registered via a custom image registration pipeline.

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Linda Decker @lindithium.bsky.social · 01/09/2026

Excited to present mechanoMaST, our workflow that spatially maps AFM-based mechancis to Spatial Transcriptomics as a preprint and in a selected talk today at the @cancergrand.bsky.social Spatial Biology Conference! BioRxiv: doi.org/10.64898/202... @diz-lab.bsky.social @biorxivpreprint.bsky.social

MechanoMaST worfklow diagram. Two directly adjacent colorectal cancer liver metastasis cryosections are undergoing a different process each. In one mechanical maps are generated via AFM. In the other one the gene expression maps are generated vis Spatial transcriptomics. The generated maps from both sections are then spatially registered. Via random forest regression and differential expression analysis the biomechanome, a list of genes associated with mechanical properties can be computed.

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Linda Decker @lindithium.bsky.social · 08/07/2025

Returning inspired for science from #LINO25. Thank you to all Nobel Laureates, Organisers, Guests and fellow Young Scientists for creating such an open and supportive environment full of excitement for meaningful research.

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