iiinova — where your digital self keeps evolving

Liuba Dvinskikh @ld-light.bsky.social · 02/04/2026

Was great to be back in Stockholm for #FOM2026 together with others from the @lasergroup.bsky.social. Enjoyed presenting recent work on lighsheet/OPM for cleared and expanded samples and learning about the latest developments in microscopy! 🔬

View of Stockholm's Old Town (Gamla Stan) from Södermalm

030

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We hope this will open up further applications for fast 3D imaging of tissues and complex structures with sub-cellular resolution, using conventional sample mounting techniques. Thank you to everyone who made this work possible. Full details 👉 doi.org/10.1364/BOE.... (🧵 6/6)

doi.org

Optica Publishing Group

031

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and > 1 cm long mouse bone 🐭🦴 (🧵 5/6).

112

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵 4/6).

121

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We demonstrate imaging of higher refractive indices (RI), maintaining a sub-1.5 µm axial resolution over hundreds of microns in depth, with multi-view image fusion providing more isotropic resolution (🧵 3/6).

101

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy using: 🔹Folded remote-refocusing OPM 🔹1.25 NA silicone-immersion primary objective (🧵2/6).

121

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

✨ Now out in Biomedical Optics Express: Cleared tissue dual-view oblique plane microscopy 🔬: doi.org/10.1364/BOE.... (🧵1/6)

doi.org

Optica Publishing Group

112

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We hope this will open up further applications for sub-cellular resolution 3D imaging of tissues and complex structures, using conventional sample preparation and mounting techniques 🔬. Thank you to everyone who made this work possible. Full details 👉 doi.org/10.1364/BOE.... (🧵6/6)

doi.org

Optica Publishing Group

000

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and >1 cm-long mouse bone 🐭🦴 (🧵5/6).

Animation of fluorescence microscopy data of a fly brain

100

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵4/6).

$Figure showing bead images for a range of sample refractive indices and correction collar positions$

100

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

Two figures, one showing orthoslices through a bead volume, the second showing the X,Y,Z FWHM variation with imaging depth

100

Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy with a folded remote-refocusing OPM system configured with a 1.25 NA silicone-immersion primary objective (🧵2/6).

Diagram of a dual-view OPM system showing the light path rays for the two views

100

Reposted by Liuba Dvinskikh

Department of Chemical Engineering and Biotechnology, Cambridge @ceb.cam.ac.uk · 11/08/2025

Last week, our Early Career Researchers (ECRs) and Postdocs Committee hosted their first-ever departmental social. These are the events where ideas spark and collaborations take root. 🧪⚙️🌱🔋 #ChemicalEngineering #Biotechnology #CambridgeUniversity @rogerrubiosanchez.bsky.social

A diverse group of people smiling, gathered in a modern lounge with red carpet and wooden walls, suggesting a casual, friendly event.

032

Reposted by Liuba Dvinskikh

FocalPlane @focalplane.bsky.social · 13/06/2025

🔬Our preprint list is now up on FocalPlane. This week we focus on new tools and techniques in imaging. Let us know if we are missing any recent preprints that you've enjoyed reading. focalplane.biologists.com/2025/06/13/m...

focalplane.biologists.com

Microscopy preprints – new tools and techniques in imaging - FocalPlane

Microscopy preprints – new tools and techniques in imaging - News

0198

Liuba Dvinskikh @ld-light.bsky.social · 26/05/2025

Hello Bluesky! 🦋 New preprint from my postdoctoral work back at @imperialcollegeldn.bsky.social - design, characterization and application of a dual-view oblique plane microscope 🔬(dOPM) for cleared tissue imaging over a range of refractive indices. www.biorxiv.org/content/10.1...

biorxiv.org

Cleared tissue dual-view oblique plane microscopy

We present a dual-view oblique plane microscope (dOPM) for imaging thick optically cleared tissue samples using a silicone immersion primary objective. The custom-designed remote refocusing relay util...

0102