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Liuba Dvinskikh

@ld-light.bsky.social

Postdoc in optical microscopy, laser.ceb.cam.ac.uk , University of Cambridge Previously Imperial College London @imperialcollegeldn.bsky.social‬ Interested in optics, biology and healthcare.

Posts Replies Media

Liuba Dvinskikh @ld-light.bsky.social · 02/04/2026

Was great to be back in Stockholm for #FOM2026 together with others from the @lasergroup.bsky.social. Enjoyed presenting recent work on lighsheet/OPM for cleared and expanded samples and learning about the latest developments in microscopy! 🔬

View of Stockholm's Old Town (Gamla Stan) from Södermalm

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and > 1 cm long mouse bone 🐭🦴 (🧵 5/6).

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵 4/6).

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We demonstrate imaging of higher refractive indices (RI), maintaining a sub-1.5 µm axial resolution over hundreds of microns in depth, with multi-view image fusion providing more isotropic resolution (🧵 3/6).

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy using: 🔹Folded remote-refocusing OPM 🔹1.25 NA silicone-immersion primary objective (🧵2/6).

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and >1 cm-long mouse bone 🐭🦴 (🧵5/6).

Animation of fluorescence microscopy data of a fly brain

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵4/6).

$Figure showing bead images for a range of sample refractive indices and correction collar positions$

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

Two figures, one showing orthoslices through a bead volume, the second showing the X,Y,Z FWHM variation with imaging depth

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Liuba Dvinskikh @ld-light.bsky.social · 02/09/2025

We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy with a folded remote-refocusing OPM system configured with a 1.25 NA silicone-immersion primary objective (🧵2/6).

Diagram of a dual-view OPM system showing the light path rays for the two views

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