iiinova — where your digital self keeps evolving

Korostelev Lab @korostelevlab.bsky.social · 05/12/2025

GTPBP1 is a GTPase implicated in neurodevelopment. Our cryo-EM maps show GTPBP1 delivering tRNA to ribosome. Due to delayed GTPase dissociation, mRNA decoding is more stringent than with bona fide delivery factor eEF1A. Congrats to Denis Susorov and collab with Pestova lab! tinyurl.com/3smdvt3v

0288

Korostelev Lab @korostelevlab.bsky.social · 30/11/2025

Using "in extracto cryo-EM", we visualize ribosomes in mammalian lysates, including RRL. "Hibernating" ribosomes carry an extended set of proteins that protect functional centers. These include elongation factor eEF2, LARP1 implicated in mTOR signaling, eIF5A etc. www.biorxiv.org/content/10.1...

12712

Korostelev Lab @korostelevlab.bsky.social · 16/06/2025

ROOL monomers entangle with other monomers via strand swapping to form octameric RNA nanocages. www.nature.com/articles/s41...

031

Korostelev Lab @korostelevlab.bsky.social · 16/06/2025

With our ‪@RTI-UMassChan.bsky.social‬ colleagues Xiaobin Ling and Wenwen Fang, we report cryo-EM structures of enigmatic bacterial RNA nanocages made of ROOL RNAs. Octamers are held by "A-minor staples" and other interactions. Congrats Xiaobin, Dima et al! www.nature.com/articles/s41...

0120

Korostelev Lab @korostelevlab.bsky.social · 16/06/2025

Activation of Retron-Septu for antiphage defense. Congrats Tian-Min and all co-authors! www.science.org/doi/full/10....

030

Korostelev Lab @korostelevlab.bsky.social · 06/12/2024

RiSANA is online! We detail the Ribosome Stimulated Angiogenin Nuclease Assay (RiSANA) to explore: (a) whether Angiogenin is activated by bacterial 70S ribosomes; and (b) whether the closest homolog RNase 4 is stimulated by 80S: tinyurl.com/3fftczrw Congrats Emily Sholi and Anna Loveland!

074

Korostelev Lab @korostelevlab.bsky.social · 22/11/2024

Activation of GCN2 relies on crossed helices at the dimer core: academic.oup.com/pnasnexus/advance-…

110

Korostelev Lab @korostelevlab.bsky.social · 09/05/2024

Angiogenin controls angiogenesis (et plus) by nicking tRNAs. But it’s barely active alone. We discovered how 80S ribosome unlocks Ang’s active site and “brings in” tRNAs. Congrats to Anna Loveland and collab. w/@ajplanetearth! www.nature.com/articles/s41586-024-…

0124

Korostelev Lab @korostelevlab.bsky.social · 04/06/2023

Congratulations to Dr. Christine Carbone on the 2023 @UMassChan Chancellor's thesis award! Her PhD work clarifies ribosome rescue (rdcu.be/b9EtA) and ribosome translocation, via time-resolved cryo-EM (rdcu.be/cC6E9). Christine, congrats on your achievements!

010

Korostelev Lab @korostelevlab.bsky.social · 08/11/2022

Congrats to Dr. Christine Carbone on exemplar thesis defense! Christine @seussofscience used cryo-EM to visualize ribosomal translocation by EF-G*GTP (rdcu.be/cC6E9) and ribosome rescue by ArfB (rdcu.be/b9EtA) – an exciting science journey full of surprises!

000

Korostelev Lab @korostelevlab.bsky.social · 01/10/2022

How do Dicer-1 and Loqs-PB make miRNA? We captured 6 cryo-EM structures showing stages of pre-miRNA cleavage. Dicer•Loqs bend pre-miRNA to cleave the right targets: www.cell.com/molecular-cell/fulltex… Fun collab. with @zamore_lab and Traci Hall...

100

Korostelev Lab @korostelevlab.bsky.social · 01/07/2022

Hot off the press: new review @AnnualReviews discusses recent time-resolved cryo-EM studies visualizing how ribosome moves and interacts with translation factors to achieve efficient and accurate translation. “The Structural Dynamics of Translation”: tinyurl.com/2ezt9st9

0125

Korostelev Lab @korostelevlab.bsky.social · 19/05/2022

GGGGCC repeat expansion in C9ORF2 gene causes neurodegeneration in amyotrophic lateral sclerosis (ALS). The repeats produce peptides, such as poly-GR and poly-PR. We show that poly-GR/PR bind the ribosome peptide tunnel and block protein synthesis: rdcu.be/cNTwi

010

Korostelev Lab @korostelevlab.bsky.social · 30/09/2021

Here is the second issue of articles and reviews dedicated to Alexander S. Spirin: link.springer.com/journal/10541/vol… Read up on informosomes, IRES RNAs and, of course, ribosomes.

000

Korostelev Lab @korostelevlab.bsky.social · 27/09/2021

Two issues of commemorative articles and reviews are dedicated to Alexander S. Spirin, an outstanding scientist and visionary, who passed away last year. Here is Part I on ribosomes, origin of life, RNA and more: link.springer.com/journal/10541/vol…

010

Korostelev Lab @korostelevlab.bsky.social · 03/08/2021

mRNA frameshifting controls expression of some proteins; viruses use it to expand their coding repertoire. Our paper with @YaMingHou1 discusses +1 frameshifting as mRNA moves through the ribosome: www.nature.com/articles/s41467-021-… Congrats to G. Demo and all...

100

Korostelev Lab @korostelevlab.bsky.social · 30/12/2020

Ever wondered how the ribosome "hops" over a nucleotide to shift to another mRNA frame? We captured cryo-EM structures showing +1 frameshifting as EF-G translocates tRNA and mRNA. Check out preprint by Gabriel Demo et al; in collab with Ya-Ming Hou's lab: disq.us/t/3tupvfl

010

Korostelev Lab @korostelevlab.bsky.social · 05/12/2020

Postdoc position open in @KorostelevLab, University of Massachusetts Medical School. An opportunity to study translation, cell stress, neurobiology by cryo-EM, in situ EM, biochemistry. For detail and application information:...

100

Korostelev Lab @korostelevlab.bsky.social · 03/11/2020

Ribosomes jam if mRNA is cut. But ArfB comes to rescue! It sneaks into mRNA tunnel and frees the peptide. We find that ArfB works on short and extended poly(A) mRNAs: here, ArfB dimer binds mRNA off the tunnel. See cryo-EM/biochem work by Carbone et al: rdcu.be/b9EtA

100

Korostelev Lab @korostelevlab.bsky.social · 31/08/2020

Expanded GGGGCC repeats in the C9ORF72 gene cause amyotrophic lateral sclerosis and frontotemporal dementia. They produce toxic repeat proteins poly-PR and poly-GR. With Fen-Biao Gao's lab, we found how these peptides bind ribosomes and block translation:...

100

Korostelev Lab @korostelevlab.bsky.social · 18/08/2020

Nanoluciferase glows right upon release from the ribosome. This can be harnessed to study how ribosomes release complete or incomplete peptides (as in premature nonsense-codon diseases), and search for read-through drugs. See manuscript by Susorov et al:...

100