iiinova — where your digital self keeps evolving

Reposted by Kole DeGolier

Marion Pepper @marionpepper.bsky.social · 22/01/2025

No functional NIH…no US scientists that spend their days making discoveries that save lives and drive US innovation and economic growth. This will negatively impact all Americans.

0127

Reposted by Kole DeGolier

E John Wherry @ejohnwherry.bsky.social · 23/01/2025

Yes. Exactly. One of (if not THE) best ROI for any govt spending.

24519

Kole DeGolier @koledegolier.bsky.social · 02/01/2025

Couldn't have done it without the help of many other people, including co-authors: Etienne Danis, Marc D'Antonio, Jen Cimons, Michael Yarnell, Eric Kohler, Ross Kedl, James Scott-Browne and my PhD advisor, Terry Fry! Also big thanks to @cuanschutz.bsky.social.

010

Kole DeGolier @koledegolier.bsky.social · 02/01/2025

We expect our findings to provide useful insights toward understanding and modulating cellular states for more effective cell therapies in cancer and other diseases.

000

Kole DeGolier @koledegolier.bsky.social · 02/01/2025

Finally, transcriptomic and epigenetic analyses reveal that the RUNX2 transcription factor is more active in memory-derived CAR T cells. Overexpressing RUNX2 in naive-derived CAR T cells enhances cytotoxicity without impairing proliferation, improving overall anti-tumor function.

000

Kole DeGolier @koledegolier.bsky.social · 02/01/2025

We show that despite this stimulation, many functional traits characteristic of the ancestral T cell state persist in the final CAR T cell populations, including enhanced cytotoxicity in memory-derived cells and superior proliferative capacity in naïve-derived cells.

010

Kole DeGolier @koledegolier.bsky.social · 02/01/2025

In CAR T cell therapy, a patient’s T cells are engineered to recognize and kill cancer cells. However, manufacturing involves strong stimulation of the T cell, raising questions about whether engineered T cells retain the differentiation states found intrinsically in T cells.

010

Kole DeGolier @koledegolier.bsky.social · 02/01/2025

This publication encompasses the bulk of my PhD thesis work to understand how the ‘history’ of a T cell impacts anti-tumor functionality when the T cell is engineered to recognize and kill cancer cells with a synthetic receptor known as a chimeric antigen receptor (or CAR).

010