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Rasmus Kirkegaard @kirk3gaard.bsky.social · 22/06/2026

I wonder if @nanoporetech.com will ever release the much faster native barcoding kit where the sequencing adapters simply have the barcodes included. Surely those 75 minutes extra could save a life one day (+ minimize DNA loss and lab frustrations)

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 08/06/2026

Is @nanoporetech.com hac v6 better than 5.2.0 sup? The short answer is no. But are a few errors in a genome worth 5 times more basecalling compute? github.com/Kirk3gaard/M...

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 22/05/2026

When you run out of slots on the P24 it is handy to have a bunch of P2 solos 🟩🟩🧬 @nanoporetech.com

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 15/05/2026

On this day in 2015 a disruptive company handed out DNA sequencers in goodie bags 🤯🤯🤯. Next week @nanoporetech.com is hosting their yearly London Calling event. Bring back disruption 🧬🚀

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 11/05/2026

The nvidia dgx spark is a cool box but you get more @nanoporetech.com basecalling for your money with one of the other options (github.com/Kirk3gaard/2...)

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 06/05/2026

Ever wondered what microbes you could find in Denmark? Pupils from Danish schools collected soil samples across the country so you can now browse the microbes (cmc-aau.github.io/masseeksperi...).

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 30/04/2026

Anyone happen to have a dgx spark doing nothing? Benchmark it for @nanoporetech.com basecalling (github.com/Kirk3gaard/2...)

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 23/04/2026

Why would @nanoporetech.com minknow produce fast5 files in 2026???

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 13/04/2026

Quite a few flowcells going beyond 200 gbp

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 20/03/2026

Will 2026 be the year we finally see something new from @nanoporetech.com or are we stuck due to the clinical focus? Next generation ASIC chips would be the opportunity to align product lines. One flowcell to rule them all?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 13/02/2026

The sun brings out the green @nanoporetech.com 🟩🟩🟩🟩🟩🚀🧬 Happy Friday everyone.

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 19/01/2026

With the P2S joining the long list of abandoned @nanoporetech.com products in favour of the expensive P2i it becomes clearer that the #anyone #anywhere vision is lost...

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 15/01/2026

Yes. These are metagenomic samples after bead beating so pretty much the sweet spot for sequencing.

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 13/01/2026

I assume it depends on who is pouring the pores onto the flowcells at the factory (randomness).

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 13/01/2026

Been doing a lot of @nanoporetech.com sequencing for soil and sediment lately and it appears that no matter how much DNA or how little we still get decent yields. So pretty robust performance. Yield clearly depends on pore count.

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 08/12/2025

One flowcell from @nanoporetech.com yielded 260 Gbp 🎉🚀🤯🟩

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 04/11/2025

Did not expect to see disk space become an issue but after a disk clean up and restart I ended up with 243 and 244 gbp for these two @nanoporetech.com flowcells 🎉🚀🧬 💚🟩💚🟩💚🟩

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 03/11/2025

After a day with almost 30 participants in the lab for our hands on @nanoporetech.com metagenomics course it is a blessing to see 🟩🟩🟩🟩🟩🟩🟩🟩🟩

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 21/10/2025

Anyone else seeing @nanoporetech.com flowcells starting to "die" exactly around 72 hours? Could this be voltage control not being optimised for longer durations?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 07/10/2025

You load one @nanoporetech.com flowcell and it almost ruins your day 💩💩💩. You load six more and they look stunning 🟩💚🟩💚💚🟩

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 24/06/2025

Have not done much @nanoporetech.com sequencing lately but this looks pretty 🟩🟩🟩🟩🟩🟩 #MFDExtreme

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 24/06/2025

Gotta catch em all! A little late night sampling for the MFD Extreme project. What microbes call this sand their home?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 21/06/2025

Beautiful evening for sampling. Microbes live in so many funky places and I finally got the chance to hunt them in locations that were not wastewater treatment plants 🎉🦠🧬🇩🇰 #MFDExtreme

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 16/06/2025

Quality scores seem to be pretty well calibrated but is a bit conservative which is probably better than being overly optimistic. Mappings with 100 % identity hits come from a wide range of quality scores

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 06/06/2025

Can we please get Dorado 1.0.0+ in @nanoporetech.com minknow ASAP? 4090 also jumps quite a bit and is getting closer to support a full P2S with live sup 🎉

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 04/06/2025

Even some pretty long reads in the 100 % identity pool.

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 04/06/2025

Got a chance to take the @nanoporetech.com hyp(er accuracy) model for a spin. Pretty decent improvement in read quality 🚀 but expect to spend quite some more GPU hours 😉 (Reads were mapped with minimap2 and identity scores from nanoplot were used for phred score calculations)

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 30/05/2025

Wandering while wondering if this sample cup will hold a story exciting enough for @isme-microbes.bsky.social conference in New Zealand next year 🦠

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 17/04/2025

Considering an apple gpu for your @nanoporetech.com basecalling? Then I hope you have lots of patience or is happy with "fast" mode data quality. github.com/Kirk3gaard/2...

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 10/04/2025

Already some nice entries added to the list for @nanoporetech.com basecalling 🎉 Do you have some nvidia GPUs that are missing?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 09/04/2025

Help your fellow researchers decide what nvidia GPU to buy for their @nanoporetech.com basecalling needs. github.com/Kirk3gaard/2...

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 27/03/2025

Okay. So I have not done the @nanoporetech.com native barcoding preps for some years. It went form including an ampure cleanup step and take forward 75 % of your DNA to no ("optional") cleanup and take forward 0.75/15ul=5 % of your DNA. Any inputs on whether yield performance is similar?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 25/03/2025

After reading recent blog posts by @omicsomics.bsky.social with the development and history of DNA seq I realized that I was missing an updated figure to summarize it in the style originally by @lexnederbragt.bsky.social (flxlexblog.wordpress.com/2016/07/08/d...) Anyone doing a 2025 edition?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 11/02/2025

Value of DNA sequencing companies dropping faster than the price per genome?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 07/02/2025

"Tesla sales dropped 60% in Germany" electrek.co/2025/02/05/t...

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 07/02/2025

Did bulk options beyond 32 flowcells magically disappear @nanoporetech.com ?

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 04/02/2025

Looking forward to see how much faster the nvidia RTX5090s will be at basecalling @nanoporetech.com data. Here SUP v5.0.0 with dorado 0.9.1 and the cards that we have available.

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 23/01/2025

Just unboxed a new @nanoporetech.com P2S. Somehow the ONT logo has moved since the first one we got. 🟩🟩🟩🟩🟩🟩🟩

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Rasmus Kirkegaard @kirk3gaard.bsky.social · 13/12/2024

Anyone have a cheap and easy to use lab solution for keeping track of reagent usage by users to support splitting bills for a multi PI lab? I am thinking scan a QR code on the cupbourd and type the number of reactions of e.g. Qubit, powersoil Pro you take.

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