iiinova — where your digital self keeps evolving

Reposted by Jean de Lima

Adrian Liston @labliston.bsky.social · 31/03/2026

A one minute primer on how #AutoSpectral improves spectral flow cytometry unmixing by using per-cell calculation of autofluroscence spectra. Use our R package and get higher quality spectral data, easy as that! www.biorxiv.org/content/10.1...

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

I’m especially grateful to my mentor @immunologyking.bsky.social for supporting a curiosity-driven science. Thanks to all collaborators! Finally, this work was made possible by @snsf.ch funding and the infrastructure within @biomedizin.unibas.ch and other facilities across the country.

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

That’s the cleaned story, but the reality was more complicated. We had to regenerate Hif1a mouse lines couple of times, the core of the story made more sense near the end of my PhD (after some “failed” projects). If you’re navigating a turbulent project and self-doubt: you’re not alone out there.

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

Finally, two days after viral #challenge, Hif1aiKO mice showed higher infectious titers, which were rescued by exogenous IL-21 treatment. A similar effect was observed in the survival of Hif1a iKO mice in a lung #adenocarcinoma model (10/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

Timing mattered! Deletion at day 15 (when HIF-1α is core-enriched) decreased lung #GC -like B cells and #IgA titers, whereas deletion at day 25 (when HIF-1α shifts to the TLS periphery) mainly affected IgA titers (9/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

IL-21 is not exclusively being sense by T or B cells! We could observe that lung #Macrophages, resident #NK cells and lung residents B cells were impaired in Hif1aiKO mice, and rescued by IL-21 (8/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

We next asked which HIF-1α–dependent signals sustain CXCR6+ TRH cells. Intratracheal #IL-21 administration restored CXCR6+ TRH cell numbers, consistent with reduced #Il21 transcripts in sorted TRH cells and lower IL-21 levels in #BALF from Hif1aiKO mice (7/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

The most affected subset? A hybrid Th1/TRH population we term CXCR6+ TRH cells (6/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

Next, we generated the Hif1aiKO mouse line, which allowed us to preserve HIF-1α activity during initial priming of CD4+ T cells. Notably, lung resident CD4+ T cells were selectively decreased in Hif1aiKO mice (5/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

Using the Egln3-YFP reporter mouse stain (generated by the Goldrath lab 🇺🇸), we tracked HIF-1α-active TRH cells. They were restricted to the lung extravascular compartment, transient during infection and localize to the borders of TLS (4/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

This echoed earlier #scRNAseq data from the lab: lung Tfh-like resident helpers (TRH) split into two subsets highly expressing Bcl6 or HIF-1α (3/10).

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Jean de Lima @jeandelima.bsky.social · 07/03/2026

Two weeks after infection, lung #TLS could be divided into inner and outer zones based on B cell proportional density. The outer zone was enriched for non-GC Tfh signatures and HIF-1α–associated pathways, such as #Hypoxia, also observed by Hypoxyprobe staining (2/10).

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Jean de Lima @jeandelima.bsky.social · 18/06/2025

Excited to share our new preprint on Hif1a in lung CD4 T cells ⏲️🥧 It’s been a challenging but rewarding journey. Huge thanks to Carolyn for her brilliant mentorship and to all our collaborators for their support!

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Reposted by Jean de Lima

Mauro Gaya @maurogaya.bsky.social · 30/04/2025

🚨🦠🔬 Last month to register to the EMBO workshop on Adaptive Immunity in Barrier Tissues. 📍 Where: Basel, Switzerland 📅 When: 26th–29th August 2025 📝 Registration deadline: 30th May 2025 Childcare support & travel grants from @embo.org and @efis-immunology.bsky.social #EMBOBarrierTissues

meetings.embo.org

Adaptive immunity in barrier tissues

Immune responses lie at the heart of almost every aspect of human health, including host responses to infection, autoimmunity, cancer, metabolism and aging. While adaptive immune responses by B and T…

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Jean de Lima @jeandelima.bsky.social · 20/12/2024

Excited for an amazing meeting in Basel, CH ❤️‍🔥 So much to learn, share, and enjoy!

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