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Innis Lab

@innislab.bsky.social

211 followers 222 following 3 posts

Molecular and structural biology lab at the ARNA laboratory in Bordeaux, France. Ribosomes, antimicrobials and bacterial adaptation. www.innislab.org

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Elodie Carmen Leroy @ecleroy.bsky.social · 21/09/2026

How can a single antibiotic halt translation across all domains of life? 🧬 Our new study in Nucleic Acids Research reveals how Sparsomycin traps active ribosomes through a conserved mechanism. academic.oup.com/nar/article/... #Ribosome #Translation #StructuralBiology #Antibiotics #RNA #iTPseq

Graphical representation of the T. thermophilus 70S ribosome showing the 30S and 50S subunits, mRNA, and P-site tRNA. Sparsomycin (yellow) binds in the peptidyl transferase center at the interface between the A and P sites. The enlarged view shows sparsomycin occupying the A-site cleft adjacent to the P-site fMet-tRNA, closely contacting its 3′ CCA end (C74, C75 and A76) and the attached fMet.

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Innis Lab @innislab.bsky.social · 17/01/2026

We’re thrilled to share our latest iTP-seq protocol for mapping bacterial translation landscapes in vitro — with a complete experimental workflow streamlined by Mélanie Gillard and an open source Python library for data analysis developed by Thibaud Renault.

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iTP-seq: a scalable profiling workflow to characterize bacterial translation landscapes in vitro

Nature Protocols - This protocol describes inverse toeprinting coupled to next-generation sequencing, an in vitro approach to characterize bacterial translation at codon resolution that can...

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