Here, we present a protocol for quantifying zebrafish vertebral column morphology using micro-computed tomography (microCT) datasets for scoliosis researchers. We describe steps for orientation of ske...

Here they combine in vivo imaging with proteomics to characterize a protein complex comprised of Ccdc78 and Ccdc33 that acts at the extreme distal tip of 9 + 2 motile cilia to control tip architecture...

The CPLANE complex is essential for ciliogenesis, and mutations to all but one subunit have been associated with ciliopathies. Here they identify three familial mutations in the final subunit, RSG1, t...

Voigt et al. show that the 5′-inositol phosphatase gene inppl1a drives notochord expansion independent of vacuole fusion. Defects in notochord expansion are exacerbated by locomotion, causing more sev...

The beating of cilia on multi-ciliated cells (MCCs) is essential for normal development and homeostasis in animals. Unlike basal bodies or axonemes, the distal tips of MCC cilia remain poorly defined....