iiinova — where your digital self keeps evolving

Louise Johnson @drlouisejohnson.bsky.social · 18/04/2026

Slow worm rescue from what I thought could be a watery grave in the pond… but apparently they are good at swimming. #wildlife #reptiles #spring #uk

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 13/02/2026

This #FluorescenceFriday we use our Ankyron CJ30442 (cyan) to identify the lymphatic marker LYVE1 in skin, showing the extensive lymphatic network through to the individual oak-leaf-shaped endothelial cell on the Mica #microscope @leicamicrosystems.bsky.social. Learn more at proimmune.com/ankyron.

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 05/12/2025

This #FluorescenceFriday we are shining a light on lymphatics in skin using Ankyron® clone DE40213, which binds Podoplanin (cyan). Image captured with our @discoverecho.bsky.social #microscope. Learn more about our recombinant antibody-replacements at proimmune.com/ankyron

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 17/10/2025

This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @discoverecho.bsky.social #microscope.

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 12/09/2025

This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @discoverecho.bsky.social #microscope. Learn more: proimmune.com/ankyron

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Louise Johnson @drlouisejohnson.bsky.social · 21/06/2025

Happy #Caturday! Princess Fifi Trixibelle Murder Mittens (first of her name) is enjoying the weekend #cats #adoptdontshop #pond

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 02/05/2025

Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope. Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron

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Louise Johnson @drlouisejohnson.bsky.social · 27/04/2025

I wish “smell-o-vision” was a thing, as these #lilyofthevalley smell incredible. #Spring #April #gardenpond #nativeplant #uk

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 25/04/2025

Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely @discoverecho.bsky.social Echo Revolve #microscope. #immunology #animalfree

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Louise Johnson @drlouisejohnson.bsky.social · 21/04/2025

Looking through the leper’s squint into a church that is approaching its 1000th birthday #uk #history #england

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Louise Johnson @drlouisejohnson.bsky.social · 12/04/2025

Happy #Caturday! Not sure how long my Easter decorations will stay in place though

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 07/04/2025

#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @discoverecho.bsky.social #microscope in colour camera upright configuration. proimmune.com\ankyron

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 04/04/2025

This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @discoverecho.bsky.social microscope. Look out for our other organelle-specific #animalfree Ankyrons™ at proimmune.com\ankyron

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Louise Johnson @drlouisejohnson.bsky.social · 28/03/2025

I disturbed another occupant of the Oxford Science Park but they were illegally fishing - goldfish saved!

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 28/03/2025

Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our @discoverecho.bsky.social #microscope

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 21/03/2025

This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope Find out more: www.proimmune.com/ankyron #cytokine @afruk.bsky.social

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 14/03/2025

#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope #bioscience #immunology #animalfreeresearch #cytoplasm @afruk.bsky.social

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 13/03/2025

Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay. doi.org/10.1208/s122... #BLI #immunology #animalfree

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Reposted by Louise Johnson

British Society for Immunology @britsocimm.bsky.social · 11/03/2025

🚨 Last chance to register for BSI Oxford and @immunologyoxford.bsky.social's Oxford Immunology Symposium! #OIS25 📅 Date: 24 March 2025 📍 Location: Oxford Mathematical Institute 🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights Register NOW: t.co/tlXp9dddqo

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 07/03/2025

It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on @discoverecho.bsky.social #microscope Find out more www.proimmune.com/ankyron #immunology #3Rs @afruk.bsky.social

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 05/03/2025

Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns! #3Rs #westernblot @afruk.bsky.social

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Louise Johnson @drlouisejohnson.bsky.social · 28/02/2025

I love being part of this project and product line. Also, continually impressed at the sharpness of the images we achieve on our (epifluorescence) Echo Revolve microscope through the digital haze reduction software

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 21/02/2025

Spectacular cell structure #FluorescenceFriday, with an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve #microscope #bioscience #immunology #3Rs @AFR_UK

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Louise Johnson @drlouisejohnson.bsky.social · 19/02/2025

For #WindowsWednesday a view of Stourhead #nationaltrust from a room not usually open to the public. The guides were amazing and really brought it to life

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 14/02/2025

Delicate red dendrites for #FluorescenceFriday - here our #animalfree Ankyron binds Tumor Necrosis Factor Receptor Superfamily member 1B (TNFR2, in red) - Nuclei counterstained with DAPI (blue) on @Discover_Echo microscope #immunology #3Rs #biomedical #research @AFR_UK

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Louise Johnson @drlouisejohnson.bsky.social · 08/02/2025

Not sure if it is a thing on this platform but happy #caturday to all who celebrate (also please check out the amaryllis that I have managed to not kill yet)

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 07/02/2025

It's February #FluorescenceFriday! Here's a beautiful image of #animalfree Ankyron™ against proliferation marker Ki67 (red) in dividing HeLa cells counterstained with phalloidin (showing actin cytoskeleton in green) @Discover_Echo #immunology #cells #biomedical #3Rs @AFR_UK

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 31/01/2025

It's #FluorescenceFriday! We used our #animalfree antibody replacement Ankyron™ (only 15kDa) specifically binding CD31 (PECAM1) fluorescently tagged & small enough to get into tight junctions of endothelial cells using Echo Revolve #microscope @Discover_Echo #immunology #3Rs

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Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 24/01/2025

Banish January blues this #FluorescenceFriday and look into the lysosomes of Dendritic cells using our animal-free antibody replacement Ankyron™ binding LAMP3 (red), with nuclei counterstained with DAPI (blue). Imaging carried out with our @Discover_Echo #microscope. www.proimmune.com/ankyron/

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Reposted by Louise Johnson

Clive McKimmie @clive-mckimmie.bsky.social · 09/12/2024

See this super cool seminar by Tineke Contort (@pasteur.fr Cambodia) that I am helping to co-chair with Tim Morrison (Colorado) THIS THURSDAY by zoom. All welcome!

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Louise Johnson @drlouisejohnson.bsky.social · 19/10/2024

Is there anybody here?! Looking for a new social media platform where people share ideas and cool pics in a non-aggressive manner. Here’s a pic I took last weekend, going back to the village in which I grew up

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