iiinova — where your digital self keeps evolving

Reposted by Elyse Stachler

pardissabeti.bsky.social @pardissabeti.bsky.social · 03/04/2025

The @SabetiLab is excited to share our EID journal paper “Establishing methods to monitor H5N1 influenza virus in dairy cattle milk.” A collaboration led by @drelyse.bsky.social and a partnership between @broadinstitute.org , Mass DPH, Mass Dept. of Agr., & the CDC bit.ly/42gKYLo

bit.ly

Establishing Methods to Monitor Influenza (A)H5N1 Virus in Dairy Cattle Milk, Massachusetts, USA

Influenza A(H5N1) Virus in Dairy Cattle Milk

1118

Elyse Stachler @drelyse.bsky.social · 28/03/2025

Want a primer on the @sabetilab.bsky.social latest publication? See the thread I wrote on the preprint 👇

022

Elyse Stachler @drelyse.bsky.social · 28/03/2025

Now live at Emerging Infectious Diseases! "Establishing methods to monitor Influenza (A)H5N1 virus in dairy cattle milk, Massachusetts, USA" wwwnc.cdc.gov/eid/article/...

wwwnc.cdc.gov

Establishing Methods to Monitor Influenza (A)H5N1 Virus in Dairy Cattle Milk, Massachusetts, USA

Influenza A(H5N1) Virus in Dairy Cattle Milk

010

Reposted by Elyse Stachler

sabetilab.bsky.social @sabetilab.bsky.social · 25/02/2025

🐦‍⬛🔬Bird Flu (H5N1) – what’s the deal? 🤔 @pardissabeti.bsky.social joined @scishow.bsky.social to break it all down—its origins, where it stands today, and why it matters. 🦠🐦 Here’s a sneak peek! 👀 Want the full story? Head to the SciShow's YouTube channel: youtu.be/5CyVi4UzKxE?... 🎥✨

085

Reposted by Elyse Stachler

sabetilab.bsky.social @sabetilab.bsky.social · 19/12/2024

What better way to kick off our Bluesky journey than with our annual holiday card? 🌟 Celebrate unity, humanity, and the milestones shaping a brighter future with us. Happy Holidays! 🎄✨ bit.ly/3Dsr6wk

096

Elyse Stachler @drelyse.bsky.social · 06/12/2024

All cattle dairy farms remain NEGATIVE for the presence of H5N1 to date! We continue to test farms monthly so that if the outbreak should spread to the state we can detect it early and help to inform interventions. (6/FIN)

010

Elyse Stachler @drelyse.bsky.social · 06/12/2024

Validating all these methods allowed us to be in the position to support government mandated testing of all 95 dairy cattle farms in Massachusetts, led by MDAR and MDPH (5/n)

110

Elyse Stachler @drelyse.bsky.social · 06/12/2024

We also generated genomes (even from ultra-pasteurized milk!) from as little as 14 H5N1 cp/uL! Hybrid selection of RNASeq libraries resulted in the most complete genomes, but had a high input need (>500 cp/uL). Below that, we had success using an Amp-Seq protocol. (4/n)

100

Elyse Stachler @drelyse.bsky.social · 06/12/2024

With these samples, we were able to fully validate both dPCR and qPCR assays for H5N1 and RNAseP Bovine as a positive process control. (3/n)

100

Elyse Stachler @drelyse.bsky.social · 06/12/2024

With the current H5N1 outbreak in US dairy 🐄, we wanted to validate PCR and sequencing methods to deploy for surveillance efforts. Since there were no positives in NE, we sourced milk from states with active outbreaks. We screened 214 cartons in total! (2/n)

100

Elyse Stachler @drelyse.bsky.social · 05/12/2024

Guilty as charged 🙋🏼‍♀️ stay tuned for the preprint drop soon though 👀

000

Elyse Stachler @drelyse.bsky.social · 04/12/2024

If I have to call carjivirus by it's government name then fine but that guy will always be crAssphage in my heart

010

Reposted by Elyse Stachler

Srijit Seal 🇺🇸🇬🇧🇩🇪🇯🇵🇮🇳 @srijitseal.com · 22/11/2024

Here's a new starter pack for researchers at the Broad Institute of MIT and Harvard!

5103

Elyse Stachler @drelyse.bsky.social · 03/12/2024

👋 blue sky! Interests include: 🦠Infectious diseases 🔎Diagnostics 🧫 Bacteriophages 💊 Antibiotic resistance 🏥 Public health 🌳Environmental engineering 💩 Wastewater

040