Duncan Kountz @dkountz.bsky.social · 30/08/2026Strange question: has anyone tried to get a winogradsky column through customs? US customs in particular but experience anywhere is welcomed 001
Reposted by Duncan KountzCameron Thrash @jcamthrash.bsky.social · 27/08/2026Multiheme selenoenzyme essential for elemental sulfur respiration www.science.org/doi/full/10.... #jcampubsscience.orgMultiheme selenoenzyme essential for elemental sulfur respirationElemental sulfur reduction is a key process in anaerobic ecosystems and the global sulfur cycle. Although elemental sulfur serves as a terminal electron acceptor in microbial respiration, the molecula... 0138
Duncan Kountz @dkountz.bsky.social · 20/08/2026I got my official notice today that I was awarded the Humboldt fellowship. And of course I accepted it. I’m really looking forward to the next two years of technically being unemployed while I conduct research as—I presume—some sort of soul-searching hobby activity 1101
Duncan Kountz @dkountz.bsky.social · 04/08/2026It kind of looks like they are wearing knee-high boots 000
Duncan Kountz @dkountz.bsky.social · 02/08/2026Sunday evening, strolling along the river Lahn, beer in hand. Still cannot escape coot 110
Duncan Kountz @dkountz.bsky.social · 25/07/2026I say this lovingly of my country of residence, but I’ve notice that it seems like a lot of German pop culture is borrowed from Britain and America with a delay of about twenty years. Here we find a band that has discovered the asthetic of circa-2009 Coldplay 110
Duncan Kountz @dkountz.bsky.social · 24/07/2026I just noticed that the images on the covers are cofactors with blue bubbles as background. Until now, I assumed they were images from Coot. Then I thought “what kind of weird protein has *all three* hydrogenase active site metallocofaftors?” 000
Duncan Kountz @dkountz.bsky.social · 24/07/2026But I like my books and I left my home library back in the US 010
Duncan Kountz @dkountz.bsky.social · 24/07/2026I got them lightly-used but it was still something like 200 euros 110
Duncan Kountz @dkountz.bsky.social · 06/07/2026Back in the US for the first time since the move to Germany. Reunited with my cat, who has settled in with my parents and seems very happy. It’s the longest time she and I have been apart in her 13 year life 040
Reposted by Duncan Kountztrisre.bsky.social @trisre.bsky.social · 06/07/20261/ Happy to share our preprint! The Mtr complex catalyses the second-to-last step of the methanogenesis and is an essential bioenergetic machine in most methanogenic archaea. Anaerobic cryo-EM of Mtr reveals a nitrogenase-like metallocluster bound at its active site! www.biorxiv.org/content/10.6... 23215
Duncan Kountz @dkountz.bsky.social · 01/06/2026I’ll take “animals I wasn’t expecting to see in Germany for $1000” 120
Duncan Kountz @dkountz.bsky.social · 14/05/2026The clue that made me go “huh?” was a figure titled “The social hierarchy of NMRs…” 110
Duncan Kountz @dkountz.bsky.social · 14/05/2026Skimming a paper about methylotrophic methanogens and the gut and kept seeing “NMR” in the figures and the text. Of course, my brain went right to “oh they have a lot of metabolite/metabolomic data”. Turns out, NMR sounds stood for “naked mole rat”, whose gut microbiome was being studied 142
Duncan Kountz @dkountz.bsky.social · 12/05/2026Drawing from my personal experience, I would suggest the issue is that you’re thinking about science at the times when you should be thinking about things burning in the oven 220
Duncan Kountz @dkountz.bsky.social · 06/05/2026That would work! I'm 95% sure I'll be at the C1 GRS/GRC--I know that I can, I just have to make a final decision in the next few days and then register. Probably won't present anything however, but I'm happy to just attend. Are you/people from your lab going? 120
Duncan Kountz @dkountz.bsky.social · 06/05/2026This issue is one of questions that you can immediately assume is discussed regularly by specialists (in this case, microbial ecologists), but would only occur to outsiders perhaps on occasion. 000
Duncan Kountz @dkountz.bsky.social · 06/05/2026I therefore edited "anoxic microbiomes" to "microbiomes in anoxic environments", which seems more precise to my intuition, but also seems like clunky wording. 310
Duncan Kountz @dkountz.bsky.social · 06/05/2026I wrote the sentence "Methanogens lie at the base of the chemical food webs of most anoxic microbiomes." But I realized that it might not be strictly correct to call a microbiome "anoxic", as that term might only describe the environment, not the microbiome per se. 200
Duncan Kountz @dkountz.bsky.social · 06/05/2026Question for the microbial ecologists out there: do you make a terminological distinction between a microbiome and its associated environment? For clarity, the next post in this thread provides the sentence I'm writing/editing that this question is related to. 230
Reposted by Duncan KountzTomas Pascoa @tomaspascoa.bsky.social · 14/04/20261/ Excited to share our preprint! 🥳 Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine www.biorxiv.org/content/10.6... 510237
Reposted by Duncan KountzAnuj @rnfr2d2.bsky.social · 14/04/2026How do “LEGO-like” electron-bifurcating modules combine to drive degradation in BTEX-contaminated ecosystems? Check out our latest preprint, where we use cryo-EM and cryo-ET to reveal how the 1 MDa BCRII complex powers extremely endergonic aromatic ring reduction. www.biorxiv.org/content/10.6...biorxiv.org 12714
Reposted by Duncan KountzScott Horton @robertscotthorton.bsky.social · 10/04/2026Jack Szostak "All of these things frighten me, and they should frighten you as well. I made the decision to create a life for myself here, because I viewed this as a place that rewarded determination, hard work, and integrity. It still has a chance to be that place again."open.substack.comI’m a Nobel Prize-winning immigrant. I can no longer recognize this country.Attacks on science and immigrants are destroying America. 5401136
Duncan Kountz @dkountz.bsky.social · 26/03/2026I just completely fell for something that I initially thought was satire because it was so crazy. On further inspection, it was actually satire. 010
Duncan Kountz @dkountz.bsky.social · 26/03/2026“Einstein was a patent clerk [not a physicist]” tells me that biology isn’t the only thing he doesn’t know anything about 000
Duncan Kountz @dkountz.bsky.social · 22/03/2026Just got an email from my grad school’s Graduate Research Fund asking me to donate. The really amusing part is that the email addresses me as “Mr. Kountz”. I mean, if there was any organization that should know that I have a PhD… 010
Duncan Kountz @dkountz.bsky.social · 22/03/2026Okay methanogen people (and particularly Methanococcus people): Does anyone have access to this publication?: www.sciencedirect.com/science/arti... If you do, could you DM me so I can send you my email (and then you could send me the pdf?)sciencedirect.comIsolation and characterization of 22 mesophilic methanococciTwenty-two strains of mesophilic methanococci were isolated from salt marsh and other intertidal sediments of the southeastern United States. All the … 100
Duncan Kountz @dkountz.bsky.social · 17/03/2026A genetic toolkit for the human gut bacterium Mediterraneibacter gnavus identifies capsular polysaccharides as a competitive colonization factor www.nature.com/articles/s41...nature.comA genetic toolkit for the human gut bacterium Mediterraneibacter gnavus identifies capsular polysaccharides as a competitive colonization factor - Nature CommunicationsHere, the authors develop tools to genetically modify Mediterraneibacter gnavus, a human gut bacterium associated with disease, showing that capsular polysaccharides are key for intestinal colonizatio... 020
Duncan Kountz @dkountz.bsky.social · 09/03/2026I mean I’m not really sympathetic to MAGAs who face social consequences for their politics, but I have to admit that in analogous contexts such a question would be strange and fairly rude 110
Reposted by Duncan KountzNadav Amdursky @amdurskylab.bsky.social · 09/03/2026📣 I was waiting for this paper for a while 🥳 Our new paper in @pnas.org shows that ATP synthase drastically alters membrane properties related to proton diffusion. Funded by @volkswagenstiftung.de www.pnas.org/doi/abs/10.1...pnas.orgATP synthase activity boosts membrane proton acceptance and lateral diffusion | PNASIn most organisms, ATP synthesis is powered by the proton motive force (pmf) and catalyzed by ATP synthase. While the chemiosmotic theory originall... 085
Reposted by Duncan KountzMax-Planck-Gesellschaft @maxplanck.de · 08/03/2026"My motto is: just do it!" We love this interview w/ Maren Nattermann, Research Group Leader @ Max Planck Institute for Terrestrial Microbiology, on Marjory Stephenson (1885–1948), pioneer of chemical microbiology as well as her own path into science www.mpg.de/26210626/mar... #IWD2026mpg.de"There isn't one 'single right way' for a career - and it doesn’t have to be a straight line"Maren Nattermann, Research Group Leader at the Max Planck Institute for Terrestrial Microbiology, on Marjory Stephenson (1885–1948): pioneer of chemical microbiology, whose work on bacterial metabolic... 0269
Reposted by Duncan KountzSven T. Stripp @stripplab.bsky.social · 04/03/2026Happy to share our new paper on proton transfer in metalloenzymes, out now in @pccp.rsc.org! Exploiting crystallography, electrochemistry, FTIR spectroscopy, and QM calculations we identified a Zundel ion in the catalytic proton tranfer pathway of [FeFe]-hydrogenase. doi.org/10.1039/D5CP... 1125
Duncan Kountz @dkountz.bsky.social · 03/03/2026I do what I can, but with spectroscopy humor added 110
Duncan Kountz @dkountz.bsky.social · 03/03/2026Maybe this is just me, but whenever someone says Mossbauer I think of the book Mossflower in the Redwall series. I can’t be the only one. 131
Reposted by Duncan KountzRibbe Hu Labs @ribbehulab.bsky.social · 03/03/2026So excited to share our cryo-EM study of the nitrogenase cofactor assembly scaffold NifEN expressed in E. coli! @ribbehulab.bsky.social @ucibiosci.bsky.social @natcatal.nature.com www.nature.com/articles/s41...nature.comStructural insights into metallocluster trafficking in the nitrogenase assembly scaffold NifEN - Nature CatalysisStructural insights into the assembly of the complex nitrogenase cofactor are scarce. Now, cryo-EM and AlphaFold analyses of NifEN, which converts the precursor (L-cluster) to a mature cofactor (M-clu... 1205
Duncan Kountz @dkountz.bsky.social · 02/03/2026Today, I inoculated a methanogen culture for the first time in at least 10 years. Believe it or not 070
Duncan Kountz @dkountz.bsky.social · 21/02/2026For red substrate, the reaction is strongly cooperative in the absence of the regulator, but loses the cooperatively when the regulator is present. For the blue substrate, the opposite: the reaction is non-cooperative in the absence of the regulator and strongly cooperative in its presence 010
Duncan Kountz @dkountz.bsky.social · 21/02/2026Pretty allosteric regulation data from a manuscript I'm writing on my PhD work. Red and blue data are from two alternative substrates. Filled symbols = absence of allosteric regulator. Open symbols: presence of allosteric regulator. X-axis is mM substrate 160
Duncan Kountz @dkountz.bsky.social · 19/02/2026Coincidentally, your similar long distance from home came to mind when I was writing this post 000
Duncan Kountz @dkountz.bsky.social · 19/02/2026I’ve had a really positive experience in Germany so far, but from time to time I get a deep feeling of sadness from being so far from home. Not a new thing for a human to experience of course 140
Reposted by Duncan KountzÁkos T Kovács @evolvedbiofilm.bsky.social · 07/02/2026Evolutionary bioenergetics of sporulation "energetic cost of sporulation in units of ATP by integrating time-resolved genome, transcriptome, and proteome profiles" #Bsubtilis #MicroSky #PNAS by @ckarakoc.bsky.social @shoestrapped.bsky.social @jaytlennon.bsky.social www.pnas.org/doi/10.1073/...pnas.orgEvolutionary bioenergetics of sporulation | PNASEnergy is required for the expression and maintenance of complex traits. In many habitats, however, free energy available to support biosynthesis i... 0174
Duncan Kountz @dkountz.bsky.social · 05/02/2026Does anyone know a good commercial source of rumen fluid in Europe (Germany in particular)? Maybe I just need to start cultivating a relationship with farmers... 001
Duncan Kountz @dkountz.bsky.social · 04/02/2026From Joe Krzycki's, Bing Hao's and @djferguson.bsky.social 's labs: Delineation of the Active Site of MtgB, a Cobalamin-dependent Glycine Betaine Methyltransferase www.sciencedirect.com/science/arti...sciencedirect.comDelineation of the Active Site of MtgB, a Cobalamin-dependent Glycine Betaine MethyltransferaseThe MttB superfamily member MtgB catalyzes the methylation of a cognate corrinoid protein with glycine betaine and representatives have been described… 010