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Dave Matus

@davematus.bsky.social

1.6K followers 1K following 42 posts

Researcher @ UC Berkeley Advanced BioImaging Center, MCB Department, Evo-devo-cell biologist, 🎷👨‍🍳🐕🐈‍⬛

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Dave Matus @davematus.bsky.social · 05/12/2025

since you made it all the way to then end - we've already made transgenic lines with the mSG from Ando et al - it's amazing! here's a movie from our Cell Observatory project at the Advanced BioImaging Center 7/7

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Dave Matus @davematus.bsky.social · 05/12/2025

At medium and high laser powers the three mSG variants (mSG(E138D) -orange, mSG from Ando et al - red, mBaoJin - green) are all very photostable as expected compared to mNG (blue) 5/7

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Dave Matus @davematus.bsky.social · 05/12/2025

but where these new FPs really shine is in photostability - we live imaged continuously for 800 frames in single FOVs 4/7

photobleaching of mNeonGreen vs. mStayGold

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Dave Matus @davematus.bsky.social · 05/12/2025

We found that the mStayGold variant published by Ando et al. was the brightest in our experiments (mSG(A) in the plot below) 3/7

plot comparing the intensity between mNG and mStayGold variants

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Dave Matus @davematus.bsky.social · 05/12/2025

We need to build lots of new transgenic lines that are both very bright and photostable, so I was excited to test membrane fusions of the three published mStayGold variants compared to mNeonGreen. We injected mRNA into zebrafish embryos and imaged at 24hpf. 2/7

single confocal images comparing mNG vs. mStayGold

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Dave Matus @davematus.bsky.social · 11/04/2025

We are looking for scientists with experience in light sheet microscopy, biological imaging, image processing and analysis to join a dedicated team of biologists, engineers and optical physicists that are pushing the boundaries of 4D in vivo imaging! Come help us make movies like this!

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Dave Matus @davematus.bsky.social · 11/04/2025

#hiring! come join us at the Advanced BioImaging Center at UC Berkeley to help us build the #CellObservatory! 1/2 aprecruit.berkeley.edu/JPF04858

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Dave Matus @davematus.bsky.social · 07/03/2025

Hello SF! #StandUpForScience2025

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Dave Matus @davematus.bsky.social · 17/01/2025

Hi Bluesky friends! My 85 year old dad is a finalist in a graphic arts contest to design a wine bottle label for his local Maryland winery - if you think his art worthy it would be awesome if you voted for Bernard! Vote here: docs.google.com/forms/d/e/1F...

An artistic image of a wine glass and grapes for a wine label contest

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Dave Matus @davematus.bsky.social · 02/01/2025

Happy 2025!

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Dave Matus @davematus.bsky.social · 29/12/2024

Continuing the tour from Faction to HumbleSea

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Dave Matus @davematus.bsky.social · 29/12/2024

Alameda beer night!

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Dave Matus @davematus.bsky.social · 19/12/2024

Gig playing with the @ucberkeleyofficial.bsky.social Cal alumni jazz sextet (I know I’m not at alum!)

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Dave Matus @davematus.bsky.social · 17/12/2024

Yesterday we had a lab bake off (citrus themed) It was close but my “elevated key lime pie” took home the title! So many key limes gave up their juice for this cause…

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Dave Matus @davematus.bsky.social · 28/11/2024

Happy turkey day to all who celebrate! We started the day out with a Pie in the Sky inspired brunch!

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