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The Dark Matter Project @darkmatterproject.bsky.social · 24/09/2026

How can genome engineering support ecosystem health? What are the ethical considerations? How is it regulated? Free webinars: 🧬 Oct 27: Science talks by Ben Novak & Beth Shapiro ⚖️ Oct 29: Ethics, regulation & governance panel w/ Art Caplan, Alex Erwin & Larisa Rudenko Register: bit.ly/46GXlTQ

Flyer for two free webinars from NYU Langone’s Center for Synthetic Regulatory Genomics: Vertebrate Genome Engineering for Ecosystem Health — Scientific Innovation (Oct 27) and Ethics, Regulation and Governance (Oct 29). Registration: https://bit.ly/46GXlTQ.

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The Dark Matter Project @darkmatterproject.bsky.social · 10/06/2026

Interested in the Science, Ethics and Regulation of Xenotransplantation? Join the next sessions in the "Rewriting Life" series! Register here: bit.ly/4urIZQH

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The Dark Matter Project @darkmatterproject.bsky.social · 15/05/2026

Synthetic Regulatory Genomics pmc.ncbi.nlm.nih.gov/articles/PMC...

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The Dark Matter Project @darkmatterproject.bsky.social · 13/05/2026

Improved vector toolkit for genome writing in mammalian cells www.biorxiv.org/content/10.6...

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The Dark Matter Project @darkmatterproject.bsky.social · 06/05/2026

Please join us for the “Rewriting Life” series kickoff webinar on May 20th, 2026, 2-3 PM (ET). Register here: nyulangone.zoom.us/webinar/register/WN_tyBjjq2WTHytv_nyovzV9w

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The Dark Matter Project @darkmatterproject.bsky.social · 29/01/2026

Mammalian genome writing: Unlocking new length scales for genome engineering www.sciencedirect.com/science/arti...

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The Dark Matter Project @darkmatterproject.bsky.social · 20/01/2026

🧬Sign up to our free online 2026 Genome Writing Workshop 🧬 www.thedarkmatterproject.org/2026-genome-...

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The Dark Matter Project @darkmatterproject.bsky.social · 29/10/2025

The final step of engineering is what we call "conversion". It includes introducing Cre enzyme that induces recombination between the 1st loxP site in mouse Taf1 intron 24, and a 2nd loxP site located in human TAF1 intron 24.

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The Dark Matter Project @darkmatterproject.bsky.social · 29/10/2025

Next, we engineered mouse embryonic stem cells (mESCs), first with a loxP site in Taf1's intron 24, followed by integration of a marker cassette (a landing pad) downstream of the gene, and finally we replaced this cassette with the 78-kb human fragment.

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The Dark Matter Project @darkmatterproject.bsky.social · 29/10/2025

We assembled this region from an XDP patient-derived BAC, using the superpower of S. cerevisiae (yeast), into a delivery-ready vector.

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The Dark Matter Project @darkmatterproject.bsky.social · 29/10/2025

Our solution? A "hybrid-convertible" allele:

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The Dark Matter Project @darkmatterproject.bsky.social · 29/10/2025

How could one engineer a mouse carrying a LETHAL allele? A thread 🧵

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The Dark Matter Project @darkmatterproject.bsky.social · 25/10/2025

One step closer to an LLM that understands novel DNA🧬 𝗜𝘁𝗲𝗿𝗮𝘁𝗶𝘃𝗲 𝗶𝗺𝗽𝗿𝗼𝘃𝗲𝗺𝗲𝗻𝘁 𝗼𝗳 𝗱𝗲𝗲𝗽 𝗹𝗲𝗮𝗿𝗻𝗶𝗻𝗴 𝗺𝗼𝗱𝗲𝗹𝘀 𝘂𝘀𝗶𝗻𝗴 𝘀𝘆𝗻𝘁𝗵𝗲𝘁𝗶𝗰 𝗿𝗲𝗴𝘂𝗹𝗮𝘁𝗼𝗿𝘆 𝗴𝗲𝗻𝗼𝗺𝗶𝗰𝘀. pubmed.ncbi.nlm.nih.gov/41125441 Congrats Andre Ribeiro-Dos-Santos and Matt Maurano!

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