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Dagan Segal

@dagansegal.bsky.social

897 followers 1.3K following 59 posts

quantitative imaging in pediatric cancer. postdoc @Danuser Lab, UTSW Find me at dagansegal.bsky.social

Dagan Segal @dagansegal.bsky.social · 28/01/2025

9/n This led us to a model where: ✳️ caveolae offer stable docking points for active PI3K to spread, inducing survival signaling in CD99 High cells. ✳️CD99 low cells don't have caveolae and must find other ways to regulate their signaling, leaving them at a disadvantage in certain contexts.

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Dagan Segal @dagansegal.bsky.social · 28/01/2025

8/n Using tools developed for quantitative 3D analysis- we saw that CD99 High cells had uniform active PI3K organization, which was lost upon knockdown of Caveolin-1!!

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Dagan Segal @dagansegal.bsky.social · 28/01/2025

6/n- The CD99 High cells also ✳️ look CRAAZZY in fish xenografts👇 ✳️show survival advantages in fish and mice ✳️show Caveolin-1 dependent survival advantage in response to chemotherapeutic drug (check out preprint for bullets 2 and 3!)

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Dagan Segal @dagansegal.bsky.social · 28/01/2025

5/n- second observation: these cells look really different also under and electron microscope! The CD99 High cells have a ton of caveolae (e.g. "little caves" along the membrane) and higher expression of the structural caveolar component Caveolin-1 .

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Dagan Segal @dagansegal.bsky.social · 28/01/2025

4/n - And also really distinct by flow cytometry targeting CD99- which means we can use flow to isolate these cells and do some cell biology! First observation 👀: these cells look really different under a microscope!

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Dagan Segal @dagansegal.bsky.social · 28/01/2025

3/n Our study started with the observation that when we passage Ewing Sarcoma cells non-enzymatically, a distinct population of cells emerges, with high expression of the glycoprotein and Ewing Sarcoma diagnostic marker CD99. Seen here by single cell transcriptomics-

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Dagan Segal @dagansegal.bsky.social · 10/12/2024

Fun!

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Dagan Segal @dagansegal.bsky.social · 10/12/2024

Thing 4 to gain attention and followers: Following the dynamic shape changes of a cancer cell over time.

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Dagan Segal @dagansegal.bsky.social · 10/12/2024

Thing 3 to gain attention and followers: This was #NotTheCover of JCB - 3D rendering of a bunch of cancer cells imaged in zebrafish, pseudocolored by morphotype (e.g. shape classification)

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Dagan Segal @dagansegal.bsky.social · 10/12/2024

Thing 2 to gain attention and followers- cancer cells expressing an actin marker xenografted into zebrafish hindbrain ventricle tend to form these crazy pseudorosette-like structures. See Segal et al. JCB 2022

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Dagan Segal @dagansegal.bsky.social · 10/12/2024

Sharing some random things I found on my desktop to gain followers: Thing 1 - segmentation of a zebrafish xenografted with human cancer cells. Red is cancer cells, green is cell death marker, blue is proliferation marker.

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