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Reposted by Stefan Oberlin

Gregor Mendel Institute of Molecular Plant Biology @gmivienna.bsky.social · 06/08/2026

New paper! A team led by @dbuendia.bsky.social and group leader Arturo Marí-Ordóñez at the GMI of the @oeaw.bsky.social investigated how plants silence potentially parasitic transposons when these “jumping genes” take over most of the genome.

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Stefan Oberlin @chnebster.bsky.social · 25/05/2026

Now researchers can do more screens at a higher scale with a higher quality output in less time and less money.

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Stefan Oberlin @chnebster.bsky.social · 25/05/2026

We think this new approach will reshape how pooled screens are performed in settings where cells are precious, expensive, or experimentally constrained - including organoids, primary cells, and in vivo settings.

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Stefan Oberlin @chnebster.bsky.social · 25/05/2026

In one genome-wide cell-sorting screen, we identified regulators of ICAM-1 expression using only ~500,000 cells - a scale that would traditionally require far larger experiments.

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Stefan Oberlin @chnebster.bsky.social · 25/05/2026

By systematically testing multiplexed CRISPRi perturbations, we found that higher numbers of perturbations per cell can actually improve screen performance while reducing the number of cells required for genome-scale discovery.

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Stefan Oberlin @chnebster.bsky.social · 25/05/2026

A project from my postdoc in the McManus lab @mcmanuslab.bsky.social at UCSF @ucsfdc.bsky.social is out today 🎉 For years, pooled CRISPR screens have operated under a central assumption: one perturbation per cell. We challenged that assumption. 🤯 www.nature.com/articles/s41592-026-03095-w

nature.com

Multiplexed perturbation enables scalable pooled screens - Nature Methods

This paper shows that high MOI sgRNA multiplexing maintains CRISPRi screen performance while enabling reduced cell numbers, challenging conventional reliance on low-MOI infections.

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