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Pereira Lab @cellreprolab.bsky.social · 31/08/2026

2/2 Don’t miss out on this opportunity to be at the forefront of immune engineering and immunotherapy. Join us in Lund, 22-23 Oct 2026! Submit your abstract here: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 31/08/2026

1/2 Today is the last day to submit your abstract for the Immunity by Design: In Vivo Immune Cell Engineering Conference!

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Pereira Lab @cellreprolab.bsky.social · 27/08/2026

2/2 With extensive experience in academic and biotech settings, Rachel Nimmo focuses on immunotherapy for cancer and autoimmune diseases, including in vivo CAR T cell therapy. Find out more in Lund 22-23 Oct! Registration and abstract submission: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 27/08/2026

1/2 We’re excited to announce our Industry Session keynote for the Immunity by Design: In Vivo Immune Cell Engineering Conference! Rachael Nimmo (former director of the Cell Technology Group at OXB).

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Pereira Lab @cellreprolab.bsky.social · 21/08/2026

2/2 How do immune cell engineering strategies progress from the lab to the clinic? Join the discussion on biotech creation, product development, and clinical manufacturing. The conference will be held in Lund 22–23 Oct. Register and submit your abstract: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 21/08/2026

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conference presents the Industry Session, featuring Lorenz Mayr (BioMedTech Consulting) Cristiana Pires (Asgard Therapeutics), and Sara Mangsbo (Uppsala University).

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Pereira Lab @cellreprolab.bsky.social · 17/08/2026

2/2 Enjoy the rest of the summer but make sure to secure your spot within the immune cell engineering network. Join us in Lund, 22-23 Oct 2026. Submit your abstract here: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 17/08/2026

1/2 Good news! ✨ The abstract submission deadline for the Immunity by Design: In Vivo Immune Cell Engineering Conference has been extended until 1 Sept 2026.

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Pereira Lab @cellreprolab.bsky.social · 14/08/2026

2/2 From drug development to nanotechnology and non-viral delivery, how can we maximize disease targeting and therapeutic efficacy? Join us in Lund 22–23 Oct for this interesting session! Register and submit your abstract: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 14/08/2026

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conference introduces Session 3: Delivery Technologies & Translation, joining Alexandra Teleki (Uppsala University), Jai Prakash (Radboud University Medical Centre), and Samir El Andaloussi (Karolinska Institutet).

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

15) 🙏🏻 A big thank you to all the funding agencies for making this possible: @ERC_Research, @HorizonEU, @Cancerfonden, @Vetenskapsradet, @fct_pt, #NextgenerationEU, @lund-university.bsky.social , @lundstem.bsky.social , LUCC, @CNC_UC, and Region Skåne.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

14) 👥 We are grateful to our collaborators Darja Schaefer, Carol Geukens, and Asgard Therapeutics for their incredible contributions!

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

13) 📖 This study was only possible because of the great effort from Pereira Lab members Nejc Arh, Ilia Kurochkin, and Beatriz Lourenço Vaz.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

12) Together, our work identifies miRNA isoform diversity as a programmable layer of cell fate control and opens new opportunities for cancer immunotherapy.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

11) Our takeaway: - miR-124 promotes early chromatin remodeling - miR-142 reinforces cDC1 identity and function - miR-142 isomiRs cooperate during cDC1 reprogramming - miR-142 enhances anti-tumor immunity of in vivo cDC1 reprogramming - mRNAs + miRNAs enable non-viral reprogramming

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

10) Combining the delivery of PIB mRNAs with miRNA mimics showed cooperative effects and initiated non-viral cDC1 reprogramming.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

9) Importantly, miR-142 improved cancer cell reprogramming and enhanced anti-tumor immunity in vivo, leading to reduced tumor growth and increased survival rates.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

8) Functionally, miR-142-enhanced cells produced more IFN-λ1, a cytokine strongly associated with cDC1 biology and anti-tumor immunity. We further identified TGFBR1 as a miR-142-regulated barrier to cDC1 reprogramming.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

7) We then analyzed the contribution of canonical and non-canonical miRNA products to cDC1 reprogramming. While miR-124-3p recapitulated miR-124’s effects, the canonical miR-142 strands did not enhance cDC1 reprogramming. Instead, multiple 5′ isomiRs cooperated to achieve the phenotype.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

6) Meanwhile, miR-124 acts by increasing chromatin accessibility and creating a permissive environment for the reprogramming factors PU.1, IRF8 and BATF3 (PIB) to activate cDC1 programs.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

5) Mechanistically, miR-142 enhances reprogramming fidelity, repressing fibroblast identity and strengthening cDC1-specific programs. This is highlighted by increased expression of key cDC1 markers, including XCR1.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

4) Two clear hits emerged: miR-124, which increased reprogramming efficiency; and miR-142, which improved cDC1 lineage fidelity. And these effects were conserved across mouse and human systems.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

3) Despite what is known for other lineages, the role of miRNAs and isomiRs in immune cell conversions remains undefined. What did we do? We screened 15 candidate miRNAs for their role in type 1 conventional dendritic cell (cDC1) reprogramming.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

2) miRNAs regulate gene expression by targeting mRNA transcripts and generate isomiRs. IsomiRs differ by only a few nucleotides at their 5′ ends, but that changes their target repertoire.

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Pereira Lab @cellreprolab.bsky.social · 13/08/2026

1) 📢 New study out in @CellReports: We uncover how microRNAs (miRNAs) and their isoforms (isomiRs) regulate immune cell specification and reprogramming. Read it here: www.cell.com/cell-reports... A thread 🧵 #microRNA #reprogramming #immunotherapy

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Pereira Lab @cellreprolab.bsky.social · 10/08/2026

2/2 Share your latest results and get a chance to be recognized with awards for Best Abstract, Poster & Flash Talk! Submit your abstract here by August 15: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 10/08/2026

1/2 ⏰Last days to submit your abstract for the Immunity by Design: In VivoImmune Cell Engineering Conference! Join us in Lund, 22-23 Oct 2026 for two days of inspiring scientific discussions and networking with leaders in immune engineering and immunotherapy.

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Pereira Lab @cellreprolab.bsky.social · 04/08/2026

2/2 How can nanotechnology transform the treatment of immune-related diseases? Come to Lund to find out from Willem Mulder’s research! Registration and abstract submission: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 04/08/2026

1/2 We’re thrilled to announce our fourth keynote speaker for the Immunity by Design: In Vivo Immune Cell Engineering Conference! Willem Mulder (Radboud University Medical Center/ Eindhoven University of Technology) is a leading researcher in immune-imaging and precision immunotherapy.

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Pereira Lab @cellreprolab.bsky.social · 28/07/2026

2/2 Where does technology meet T-cell immunotherapy? Join us in Lund 22–23 Oct to learn about Sine Reker Hadrup’s research! Registration and abstract submission: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 28/07/2026

1/2 We’re happy to announce our third keynote speaker for the Immunity by Design: In Vivo Immune Cell Engineering Conference! Sine Reker Hadrup (Technical University of Denmark) develops technological platforms to study T cell reactivity in disease contexts.

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Pereira Lab @cellreprolab.bsky.social · 23/07/2026

4/4 Integrated into a network of exciting immune engineering projects (biohub.org/blog/immune-...), we aim to enable safe and precise in vivo immune engineering. We greatly appreciate the support from Biohub & the @chanzuckerberg.bsky.social Initiative. Reach out if you're at the symposium!

biohub.org

The immune cell engineers

Meet the scientists building tomorrow's medicine at Biohub.

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Pereira Lab @cellreprolab.bsky.social · 23/07/2026

3/4 Joining efforts with Dr. Axel Hyrenius-Wittsten, our project is advancing a synthetic immunotherapy that couples systemic delivery with local immune activation.

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Pereira Lab @cellreprolab.bsky.social · 23/07/2026

2/4 This project establishes a synthetic “sense-and-reprogram” platform using synNotch-engineered monocytes that activate dendritic cell-instructing transcription factors only upon entering tumors.

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Pereira Lab @cellreprolab.bsky.social · 23/07/2026

1/4 The Pereira Lab is at the New York Symposium on Immune Cell Engineering and Reprogramming (@biohub.org) This is an opportunity to connect with the immune engineering network & to celebrate our awarded project “A ‘sense-and-reprogram’ platform for systemic cancer immunotherapy”.

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Pereira Lab @cellreprolab.bsky.social · 22/07/2026

2/2 Learn from leading experts how synthetic immunology, T-cell engineering, and gene editing are creating new therapeutic opportunities to reach more patients. Join us in Lund 22–23 Oct for this exciting session! Register and submit your abstract: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 22/07/2026

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conference introduces Session 2: Synthetic Biology & Gene Editing, joining William Nyberg (Karolinska Institutet), Axel Hyrenius-Wittsten (Lund University), and Mirco Friedrich (DKFZ German Cancer Research Center).

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Pereira Lab @cellreprolab.bsky.social · 14/07/2026

2/2 Join us in Lund 22–23 Oct to get to know the amazing SciLifeLab community! You will find out how advanced infrastructures can help translate innovative research in immune cell engineering into next-generation therapies. Registration and abstract submission: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 14/07/2026

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conference will have a dedicated @scilifelab.se session with Tina Catela Ivkovic (Center for Translational Genomics), Mikael Mattsson (Drug Discovery and Development), and Anahita Bakochi (BioMS).

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Pereira Lab @cellreprolab.bsky.social · 08/07/2026

From reprogramming immune cells in vivo to translating discoveries into the clinic, next-generation immunotherapies are taking roots! Join us in Lund 22–23 Oct to learn more and be a part of this session! Registration and abstract submission: immunitybydesign.se #Immunotherapy #TranslationalMedicine

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 08/07/2026

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conference introduces Session 1: Cell Engineering & Reprogramming. The Pereira lab will share the stage with Judit Svensson Arvelund and Özcan Met to discuss emerging approaches in immune cell engineering and cancer immunotherapy.

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Pereira Lab @cellreprolab.bsky.social · 30/06/2026

2/2 Can we engineer immunity directly inside the body to tackle diseases ranging from cancer to autoimmunity and viral infections? Join us in Lund 22–23 Oct to learn more! Registration and abstract submission: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 30/06/2026

1/2 We’re thrilled to announce Adi Barzel (Tel Aviv University) as a keynote speaker for Immunity by Design: In Vivo Immune Cell Engineering Conference! His work explores in vivo genome editing and strategies to engineer T and B cells for therapeutic applications.

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Pereira Lab @cellreprolab.bsky.social · 26/06/2026

2/2 How can we use systems immunology to control immune cell function? Join us in Lund 22–23 Oct to meet Sai Reddy and find out! Registration and abstract submission: immunitybydesign.se

immunitybydesign.se

Immunity By Design | The Invajo conference site

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Pereira Lab @cellreprolab.bsky.social · 26/06/2026

1/2 We’re excited to announce our first keynote speaker for Immunity by Design: In Vivo Immune Cell Engineering Conference! Sai Reddy (ETH Zurich) works on engineering the immunogenome & reprogramming immune cells for applications in biotechnology, vaccination & immunotherapy.

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Pereira Lab @cellreprolab.bsky.social · 17/06/2026

2/2 If you work on immune engineering, next-gen immunotherapies, delivery technologies, or translational research, this is your meeting! ⌛️Abstract deadline: 15 August. 🏆Awards for Best Abstract, Poster & Flash Talk. 🎯Submit your abstract here: immunitybydesign.se

immunitybydesign.se

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Pereira Lab @cellreprolab.bsky.social · 17/06/2026

1/2 Registration is OPEN for the Immunity by Design: In Vivo Immune Cell Engineering Conference!✨ Join us in Lund 22–23 Oct 2026 in a vibrant environment that brings together researchers from all career stages. #ImmuneEngineering #Immunotherapy #BiotechIndustry

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Pereira Lab @cellreprolab.bsky.social · 17/05/2026

Last chance to apply for the DDLS PhD student positions! 🚨 Deadline is today. Don’t miss the opportunity! #PhD #DDLS

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Pereira Lab @cellreprolab.bsky.social · 07/05/2026

4/4 ⭐️The framework is broadly adaptable, making it applicable across reprogramming systems and boosting accessibility to everyone aiming to seize the potential of direct reprogramming to generate therapeutic cell types.

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Pereira Lab @cellreprolab.bsky.social · 07/05/2026

3/4 The workflow includes lentiviral production, arrayed library transduction, reprogramming, cell purification, library preparation, sequencing, a downstream analysis pipeline, with quality control and troubleshooting guidance throughout.

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