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breesheyrh.bsky.social

@breesheyrh.bsky.social

Reposted by @breesheyrh.bsky.social

Chris Ponting @cgatist.bsky.social · 21/01/2025

1960 ASBs are strong candidates for causally altering human traits: they’re in ChIP-Seq peaks & strengthen/weaken TF motifs in agreement with TF affinity change & are evolutionarily conserved & enriched for trait associations & eQTLs Fab collaboration w/ Breeshey Roskams-Hieter & Øyvind Almelid 2/2

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Reposted by @breesheyrh.bsky.social

Chris Ponting @cgatist.bsky.social · 21/01/2025

What DNA variants alter transcription factor (TF) binding affinity? Here, we describe a new workflow baal-nf. We used it to trawl through 6017 ChIP-Seq data sets for 558 TFs and 46 genotyped cell lines, finding 298,783 allele-specific binding sites (ASBs). www.biorxiv.org/content/10.1... 1/2

Description of image: Workflow for identifying high-quality ASBs through baal-nf. ChIP-sequencing data from genotyped cell lines is used to infer ASBs at heterozygous SNPs using BaalChIP. Identified ASBs are further characterized by mapping them to JASPAR and NoPeak motifs for a given TF. High-quality ASBs show concordance in the corrected allelic ratio and motif score difference.

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