Fibrils formed by 40- and 42-residue amyloid-β peptides (Aβ40 and Aβ42) are polymorphic, containing molecular structures that vary with growth conditions in ways that are not fully understood. Here,…

Lamin A/C proteins, integral to the nuclear lamina and present throughout the nucleoplasm, contribute to the mechanical stability of the nucleus and influence transcription as seen in laminopathies…

The artwork featured on the cover of the October 6 issue of Biophysical Journal depicts coherently diffracting domains (CDDs) within protein crystals. The brightly illuminated gray regions represent h...

DNA packaging imposes fundamental physical constraints on genomes across the tree of life. However, most of our mechanistic understanding of these processes comes from the eukaryotic nucleosome,…

Laser-induced thermal shock is widely encountered in neuroscience and biology, yet its effects on cellular thermodynamics remain poorly understood. Using whole-cell patch-clamp recordings paired with…

The Ras subfamily is the most extensively studied branch of the Ras superfamily, with 20% of all human tumors having activating mutations in one of the RAS genes. Recent studies have shown that the…

Controlling the physicochemical properties of nanoparticles is important for their performance as drug carriers, pharmaceuticals, or imaging contrast agents in nanomedicine. Predictive models can…

Heat shock protein 70 (Hsp70) is a molecular chaperone that plays a key role in cellular processes by assisting protein folding and preventing aggregation. During client-protein folding, Hsp70…

Fibrinogen plays a central role in the physiological processes of blood coagulation and, unfortunately, ischemic stroke, where it is routinely exposed to mechanical forces. In this study, we employed…

In vivo chemical cross-linking combined with mass spectrometry analysis (XL-MS) enables the direct capture of protein-protein interactions (PPIs) within their native cellular environment. This…

RyR2 channels regulate Ca2+ release from the sarcoplasmic reticulum (SR), the Ca storage organelle in cardiac muscle. Altered RyR2 activity is a key factor promoting atrial and ventricular…

Protein function-conditioned generative modeling can unify two core goals in protein engineering: predicting the effects of sequence variants and designing new sequences that satisfy functional…

Biomolecular condensates formed by phase separation inside cells often exhibit viscoelastic behavior, yet their shape recovery and fusion dynamics are frequently interpreted using purely viscous…

Deciphering the mechanisms of protein-protein interactions (PPIs) and protein structural changes within the native cellular environment is crucial for advancing drug discovery. In vivo chemical…

The NBAR-domain-containing protein endophilin, a major player in many endocytic pathways, has offered considerable insight into Bin/amphiphysin/Rvs (BAR)-domain-driven membrane remodeling. However,…

Macromolecules, such as proteins, antibodies, nanobodies, and other affinity binders, play essential roles in therapeutic and diagnostic applications due to their high specificity and functionality.…

Human skin wound healing is a complex process of tissue pattern formation driven by massive cell migration and regeneration, coordinated through intercellular communication. A multiscale tissue…

Traditionally, cells have been classified by their type. Identifying cell types was deemed vital for understanding their biological processes. More recently, cell-type classification has been…

The bacterial flagellar motor drives cell motility by rotating the flagellar filament and converting ion flux into mechanical work. At low counterclockwise (CCW) rotational speeds, the motor operates…

Histones provide one of biology’s most familiar solutions to the problem of organizing DNA. In eukaryotes, DNA wraps around histone proteins to form nucleosomes, creating the basic structural unit of ...

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